Ultra-high-pressure liquid chromatography

Silica monoliths have been applied to peptide separations [17,18] as well as to small-molecule pharmaceutical development samples [16,19], [Pg.777]

Monolithic columns do have disadvantages. Although very high flow rates are used to speed up separations, this generates a considerable amount of solvent waste for 4.6-mm-bore columns. The number of phases and column sizes is very limited at present, as is the number of commercial manufacturers. Also, the technology of particle-packed columns is not static, but continues to improve as well. Monolithic columns have not yet demonstrated the performance capabilities exhibited by sub-2-pm particles and UHPLC. However, advances in monolithic column technology in the years to come promise to bridge that gap. [Pg.777]

In 1997, the laboratory of James Jorgenson at the University of North Carolina [20, 21] was the first to demonstrate this approach by introducing [Pg.777]

Bijlsma L, Sancho JV, Pitarch E, Ibanez M, Hernandez F (2009) Simultaneous ultra-high-pressure liquid chromatography-tandem mass spectrometry determination of amphetamine and amphetamine-like stimulants, cocaine and its metabolites, and a cannabis metabolite in surface water and urban wastewater. J Chromatogr A 1216(15) 3078-3089... [Pg.224]

UHPLC Ultra-High-Pressure Liquid Chromatography... [Pg.15]

UHPLC Ultra-high-pressure liquid chromatography... [Pg.322]

Henchiz Y. Guillarme D. Martel S. Rudaz S. Veuthey J-L. Carrupt P-A. Fast log P determination by ultra-high-pressure liquid chromatography coupled with UV and mass spectrometry detections. Analytical andBioanalytical Chemistry, 2009, 394, 1919-1630. [Pg.67]

Marin JM, Gracia-Lor E, Sancho JV et al (2009) Application of ultra-high-pressure liquid chromatography-tandem mass spectrometry to the determination of multi-class pesticides in environmental and wastewater samples Study of matrix effects. J Chromatogr A 1216 1410-1420... [Pg.392]

Hasselstrom J (2011) Quantification of antidepressants and antipsychotics in human serum by precipitation and ultra high pressure liquid chromatography-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 879(1) 123—128. doi S 1570-0232(10)00727-0 [pii] 10.1016/j. jchromb.2010.11.024... [Pg.194]

For enhanced throughput, fast RPLC methods using monolithic silica columns [238], small size particles (3 pm) packed columns [173, 195, 226, 235-237, 239, 240], ultra high pressure liquid chromatography (UHPLC) columns packed with sub-2 pm particles [145,196, 227, 245, 247] and 2.6 pm core-shell particles HPLC columns [246] have been proposed for the high-throughput separation and quantification of tamoxifen/metabolites. [Pg.232]

The development of ultra high-pressure liquid chromatography technology has dramatically reduced the analysis time for a number of small molecules. However, we have found that the analysis of carotenes is poor with the currently available column chemistries. The development of C30 columns with backbones that can withstand ultra high pressure would allow carotenoid chemists to take full advantage of this technology. [Pg.135]

Aguilera-Luiz MM, Vidal JLM, Romero-Gonzalez R, Frenich AG, Multi-residue determination of veterinary drugs in milk by ultra-high-pressure liquid chromatography-tandem mass spectrometry, J. Chromatogr. A 2008 1205 10-16. [Pg.148]

Pereira L, Blythe C, Sherant R, Ritchie H. 2006. Use of small particles in ultra high pressure liquid chromatography. Available at http //www.thermo.com/eThermo/ CMA/PDFs/Various/File 30599.pdf. [Pg.364]

Wang J, Du Z, Yu W, Qu S. Detection of seven pesticides in cucumbers using hollow fibre-based liquid-phase micro-extraction and ultra-high pressure liquid chromatography coupled to tandem mass spectrometry. J Chromatogr A 2012 1247 10-17. [Pg.401]

Lee, S., Eom, H.-S., Yoo, M., Cho, Y, Shin, D. (2011). Determination of biogenic amines in Cheonggukjang using ultra high pressure liquid chromatography coupled with mass spectrometry. Food Science and Biotechnology, 20, 123-129. http //dx.doi.org/ 10.1007/S10068-011-0017-6. [Pg.304]

TISP, turbo ion spray MRM, multiple reaction monitoring UHPLC, ultra-high-pressure liquid chromatography TOP, time-of-flight mass analyzer ISP, ion spray SRM, selected reaction monitoring ESI, electrospray interface APCI, Atmospheric pressure chemical ionization. [Pg.2071]

The use of LC with conventional LC columns (5 jm) is a common and well-established technique in food analysis. However, the limitations of the use of this type of column do not solve all analytical problems. This fact is especially critical in food metabolomics because of the complexity of food matrices and the high number of compounds of interest. Therefore, the use of ultra-high-pressure liquid chromatography (UHPLC) can overcome the limitations of conventional LC, providing... [Pg.52]

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