Nucleotide binding

Pai, E.F., et al. Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation. Nature 341 209-214,... 

FIGURE 10.13 Some of the sequence homologies in the nucleotide binding and phosphorylation domains of Na, K -ATPase, Ca -ATPase, and gastric H, K -ATPase. (Adapted from j0rgensm, P. L., and Andersen, J. R, 1988. Structnral basis for Ei - E2 confoyinational transitions in Na, K -pnmp and Cc -pnmp proteins. Journal of Membrane Biology 103 95-120)... 

The Gly-X-X-Gly-X-Gly found in this segment is found in many ATP- and nucleotide-binding enzymes. The Lys of this segment is thought to interact with the a-phosphate of bound ATP. 

ABC Transporters. Figure 1 Structure of ABCB 1, ABCC1, and ABCG2 (NBF nucleotide binding fold TMD transmembrane domaine. Modified according to www.iwaki-kk.co.jp/bio/specialedition/se02.htm). 

The breast cancer resistance protein (BCRP) belongs to the G-branch of the ABC-transporter family (ABCG2). In contrast to most other ABC-proteins, BCRP consists of only one transmembrane domain (TDM) with one nucleotide binding fold (NBF) at its C-terminus. Because of this structural characteristic BCRP as well as other ABC-transporters with only one TMD are termed half transporters. To achieve functional activity these transporters have to form hetero- or homodimers. BCRP is involved in the multidrug resistance of certain tumors and transports endogenous compounds like cholesterol and steroid hormones. 

Latrunculins A and B are macrolides from the sponge Latrunculia magnified. Latrunculin A (>50 11M) binds close to the nucleotide binding site of G-actin and blocks the assembly with F-actin without promoting disassembly. 

Cyclic Nucleotide-binding Domain Cyclic Nucleotide Phosphodiesterases Cyclic Nucleotide-regulated Cation Channels Cyclic Nucleotides Cyclin... 

Metal complexes of nucleic acid derivatives and nucleotides binding sites and structures. L. G. Marzilli, Adv. Inorg. Biochem., 1981, 3, 48-85 (138). 

Actin Polymerization Regulation by Divalent Metal Ion and Nucleotide Bindings ATP Hydrolysis and Actin Binding Proteins... 

The Critical Concentration Increases with the Number of Filaments in the Presence of ATP 49 Stereochemistry of Nucleotide Binding to Actin and Tubulin Role of... 

STEREOCHEMISTRY OF NUCLEOTIDE BINDING TO ACTIN AND TUBULIN ROLE OF DIVALENT METAL ION IN NUCLEOTIDE BINDING AND HYDROLYSIS... 

Kinosian, H.J., Selden, L.A., Estes. J.E., Gershman, L.C. (1993). Nucleotide binding to actinication dependence of nucleotide dissociation and exchange rates. J. Biol. Chem. 268, 8683-8691. 

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