Mass spectrometry combined with liquid chromatography

LC-MS Liquid chromatography combined with mass spectrometry... 

Explores strategies for optimizing techniques such as high-performance liquid chromatography combined with mass spectrometry (HPLC-MS) or tandem mass spectrometry (HPLC-MS-MS)... 

Yttri, K. E., Dye, C., Slordal, L. H., and Braathen, O. A., Quantification of monosaccharide anhydrides by liquid chromatography combined with mass spectrometry Application to aerosol samples from an urban and a suburban site influenced by small-scale wood burning. Journal of the Air and Waste Management Association 55(S), 1169-1177, 2005. 

Electrochemical detection has also been suggested for determination of underivatized aminoglycosides in edible animal products (3, 19), while laser-based polarimetric detection has also been used in the analysis of underivatized gentamicin in milk (9). Liquid chromatography combined with mass spectrometry... 

Over the past decade, liquid chromatography combined with mass spectrometry (LC/MS) has evolved from being primarily a research tool, available in a limited number of analytical laboratories, to a robust and widely available analytical and investigative technique (1 3). It is now in widespread use in pharmaceutical, food, environmental, and forensic laboratories and is an indispensable tool in biomedical research. At the time of our previous review of this subject (4), few laboratories were using LC/MS in the analysis of chemicals relevant to the Chemical Weapons Convention (CWC). This was due to a number of factors, two of which were the cost of instrumentation and a perception that the technique was difficult to implement in an analytical laboratory. In the intervening years, the costs of basic LC/MS have been reduced, and the instrumentation has become robust and easier to operate. LC/MS is now a mature technique that should be considered by all laboratories that are required to analyze chemicals related to the CWC. 

LC/MS Liquid Chromatography Combined With Mass Spectrometry... 

Liquid chromatography combined with mass spectrometry (Chapter 21) is widely used for rapid and selective drug analysis, and samples can be run in 1 to 3 min. So, faster ways of sample cleanup are needed for processing large numbers of samples. 96-Well plates with small wells (so-called micro titer plates) are widely used for processing large numbers of samples in automated instruments. 

A number of suitable immunoassays and radioimmunoassays are available for measuring the major hormones testosterone, estrogen, LH, and FSH. Some of these assays may need adjustments to ensure that the majority of values fall within the optimal part of the measuring range because the ranges differ between laboratory animal species. Given the similarities in the structures of FSH and LH, some assays can be used for both rats and mice if the antisera are suitable (Jarrell et al. 1988). A number of methods have been described that use gas liquid or liquid chromatography combined with mass spectrometry, which can be used to measure several of the steroid hormones. The development of various contraceptives has often been helped by these hormone measurements as biomarkers of efficacy rather than markers of toxicity in animal models. 

J. Enebro, D. Momcilovic, M. Siika-aho, and S. Karlsson, Liquid chromatography combined with mass spectrometry for investigation of endoglucanase selectivity on carboxymethyl cellulose, Carbohydr Res., 344 (2009) 2173-2181. 

Reversed-phase high-performance liquid chromatography Scoville heat unit Solid-phase micro extraction Ultrasound-assisted extraction Ultra-high-performance liquid chromatography Ultra-performance liquid chromatography combined with mass spectrometry Ultraviolet... 

Godin, J.R, Hau, I, Fay, L., Hopfgartner, G. (2007) Liquid chromatography combined with mass spectrometry for 13C isotopic analysis in life science research. Mass Spectrometry Reviews, 26, 751- 11A. 

This review provides an up-to-date overview of the application of analytical procedures based on MS for the characterization of organic natural materials in archaeological and historical objects. Applications that feature the use of gas chromatography/mass spectrometry (GC/MS), Py-GC/MS, high performance liquid chromatography combined with mass spectrometry (HPLC/MS), and direct MS analysis such as matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), electrospray ionization mass spectrometry (ESI-MS), and direct exposure mass spectrometry (DE-MS) are sununarized to highlight the different information provided by each of the various analytical approaches. Case studies and examples are also presented and include a description of the molecular markers and of the molecular profiles that are used to identify the original materials. 

Dugo P, Kumm T, Crupi ML, Cotroneo A, MondeUo L. Comprehensive two-dimensional liquid chromatography combined with mass spectrometry detection in the analyses of triacylglycerols in natural lipidic matrixes. J Chromatogr A 2006 1112 269-75. 

MondeUo L, Tranchida PQ, Stanek V, Jandera P, EXigo G, Dugo P. Siver-ion-reversed-phase comprehensive two-dimensional liquid chromatography combined with mass spectrometry detection in lipidic food analysis. J Chromatogr A 2005 1086 91—8. 

Beck, A., Bussat, M.-C., Zorn, N., Robillard, V. and Klinguer-Hamouii C., et al. Characterization by liquid chromatography combined with mass spectrometry of monoclonal anti-IGF-1 receptor antibodies produced in CHO and NSO cells. J. Chromatogr. B 819 203-218, 2005. 

Great advances in elucidating the variability of the lipophilic ceramide portion of the glycosphingolipids have been achieved by introducing new and refined methods of gas-liquid chromatography combined with mass spectrometry. 

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