L929 mouse fibroblast cells

Cytotoxicity The cytotoxicity of nanocapsules was investigated against L929 mouse fibroblast cells and human polymorphonuclear PMNC cells with MTT assay [90], Cell viability values of different nanocapsule and nanosphere formulations on L929 and PMNC cells indicated that nonsurfactant P-CDC6 nanocapsules were less cytotoxic than nanocapsules containing surfactants. The cytotoxicity of the nanoparticles mostly arises from surfactant presence and was concentration dependent [90]. 

L929 mouse fibroblast cells in this device changed their morphology due to the forces exerted on them. A gradient was created by introducing a standard culture medium with fetal bovine serum (FBS) at one inlet and a serum-free medium at the other. FBS contains proteins, such as serum albumin, that are needed for cell survival, division and growth. The cell density and attachment depended on a combination of sheer stress and FBS concentration [28]. 

Tuscolid 65 and tuscoron A 66 were tested for biological activity against a broad spectmm of bacteria, yeasts, and fungi, but they proved to be inactive. For both compounds, no cytotoxicity was observed in assays with L929 mouse fibroblast cell culture. 

In vitro cell culture tests with plasticiser and PVC compound showed these to be non-toxic with L929 mouse fibroblast cells. 

Figure 10. A confluent monolayer (lOOx magniflcation) of well defined L929 mouse fibroblast cells exhibiting cell-to-cell contact. This appearance is indicative of a noncytotoxic (negative response in the elution test) method(Reproducedfrom reference 1. Copyright 1998 Medical Device Diagnostic Industry.)...

In this study, biodegradable amphiphilic PCL-PEG-PCL copolymer was synthesized. In aqueous medium, this amphiphilic copolymer can form nano-sized micelles. The properties of nanoparticles as drag carriers were investigated, including CMC values and particle size. Moreover, the biocompatibility of polymeric nanopaiticles was also evaluated in vitro. The cytotoxicity was examined in L929 mouse fibroblasts cell line. Furthermore, nitric oxide (NO) production and reactive oxygen species (ROS> generation were also demonstrated. 

Fig. 14 Top Representations of a PtOEGMA-co-MECLMA) coating at 37°C (a) and 25°C (b), and the corresponding cell response. Bottom. Phase-contrast microscopy images of L929 mouse fibroblasts on P(OEGMA-co-MEC>2MA)-modified gold substrates after 44 h of incubation at 37°C (left), and 30 min after cooling the sample to 25°C (right). Scale bars 100 pm. Reprinted, with...

Recently, we looked at the ability of methotrexate, tetrachloroplatinate, a physical mixture of methotrexate and tetrachloroplatinate and a methotrexate-platinum polymers to inhibit various viruses. A related study employing tilorone and a tilor-one derivative and cisplatin polymeric derivatives was carried out. The results will be briefly reported later. For these studies each ceU line is especially chosen to be compatible to support growth for the particular virus. DSC-1 cells are African green monkey kidney epithehal cells, mouse L929 are fibroblast cells, vero cells are African green monkey kidney epithelial cells, and human 143 cells are fibroblast bone osteosarcoma cells. 

Apart from the concentrafion of sericin, its extraction method has been shown to influence cell viability, also. If compared to heat, acid, or alkaline extraction methods, urea-extracted silk sericin is linked to the lowest cell viability of L929 mouse fibroblast (Aramwit et al., 2010). Sericin can also be used to replace bovine semm in cell freezing media. It cryopreserves cells as effectively as standard freeze medium containing foetal bovine serum. This effect has been demonstrated for various cell lines including P3U1 myeloma cell line, Chinese hamster ovary CHO cells, human dermal fibroblasts, human epidermal keratinocytes, the rat phaeochromocytoma cell line PC 12 and Sf9 insect cells (Sasaki et al., 2005), human primary hepatocytes (Miyamoto et al., 2010), rat pancreatic... 

Wool and grain (wheat and barley) dusts stimulated TNF secretion by rat alveolar macrophages in vitro in a dose-dependent manner as measured in supernatants with the mouse fibroblast cell line L929 bioassay (Brown and Donaldson 1996). 

Ma et al. studied the effea of organic-soluble chitosan/PHB fibers for skin regeneration that was prepared by electrospinning.The cytotoxic study was evaluated with mouse fibroblast cells (L929) and the cell culture results revealed that it benefits promoting the cell attachment and proliferation and can be used as tissue engineering for skin regeneration. 

The biocompatibility of polypyrrole has been assessed in vitro and in vivo as an effective guidance channel for the regeneration of nervous tissue and as a method of conducting current to enhance the repair of a nerve [115], A PPy coating of 25 ttm was electrochemically deposited on a platinum wire. In vitro, the cell responses from L929 mouse fibroblast and neuro2a neuroblastoma cells to the polymer coatings were evaluated under a constant current. The results indicated that the polypyrrole was cytocompatible in vitro if prepared by appropriate extraction techniques. After polymerization, extraction in methanol for a period of 1 week was carried out to remove residual electrolyte. Some evidence of toxicity was evident when a current of 1 mA was applied across the polymer for periods up to 96 h. In vivo results show there was only minimal response from tissue after 4 weeks of implantation in a rat model. 

---