African green monkey kidney epithelial

Figure 12.9 Endothelial cell-specific nuclear import of plasmids. Growth-arrested African Green Monkey kidney epithelial cells (TC7), human pulmonary artery smooth muscle cells (HSMCs) and human umbilical vein endothelial cells (HUYECs) were microinjected in the nucleus (top) and cytoplasm with CMV-driven, GFP-expressing plasmids containing either no additional sequences (open bars), the SV40 enhancer (striped bars), or the flk-1 promoter (shaded bars). Eight hours after injection, the cells were visualized for GFP expression by fluorescence microscopy. Whereas all three plasmids supported GFP expression when delivered into the nucleus of all three cell types, only the SV40 enhancer mediated nuclear import and gene expression in all cells when injected into the cytoplasm. As predicted, the flk-1 promoter caused import and expression only in cells in which transcription factors necessary for its expression and import were made, namely endothelial cells.

Vero African green monkey kidney Epithelial... 

African green monkey kidney epithelial (Vero) cells (CCL-81 American Type Culture Collection, Manassas, VA). 

Recently, we looked at the ability of methotrexate, tetrachloroplatinate, a physical mixture of methotrexate and tetrachloroplatinate and a methotrexate-platinum polymers to inhibit various viruses. A related study employing tilorone and a tilor-one derivative and cisplatin polymeric derivatives was carried out. The results will be briefly reported later. For these studies each ceU line is especially chosen to be compatible to support growth for the particular virus. DSC-1 cells are African green monkey kidney epithehal cells, mouse L929 are fibroblast cells, vero cells are African green monkey kidney epithelial cells, and human 143 cells are fibroblast bone osteosarcoma cells. 

Non-polarising BHK CHO Cos-1, Cos-7 HEK-293 (Baby) Syrian hamster kidney, fibroblast-like Chinese hamster ovary, fibroblast-like African green monkey kidney, fibroblast-like Human embryonic kidney, epithelial... 

MTT test was done after 3 days on tumor cells (ceU line established from a glioblastoma multiform grade IV in our laboratory and named Til, 20-25 passage) and Vero cells (from the Vero lineage derived from epithelial cells of kidney from African green monkey, 20-25 passage), cultured with different concentrations of... 

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