Platinum methotrexate polymer

Recently, we looked at the ability of methotrexate, tetrachloroplatinate, a physical mixture of methotrexate and tetrachloroplatinate and a methotrexate-platinum polymers to inhibit various viruses. A related study employing tilorone and a tilor-one derivative and cisplatin polymeric derivatives was carried out. The results will be briefly reported later. For these studies each ceU line is especially chosen to be compatible to support growth for the particular virus. DSC-1 cells are African green monkey kidney epithehal cells, mouse L929 are fibroblast cells, vero cells are African green monkey kidney epithelial cells, and human 143 cells are fibroblast bone osteosarcoma cells. 

In summary, methotrexate, the mixture, and the polymer are aU active against HSV-1 and VZV viruses whereas the tetrachloroplatinate showed little or no activity against any of the tested viruses. The best inhibition, that is inhibition at the lowest concentration, was found for the polymer and the mixture consistent with there being some cooperative effect of the methotrexate and platinum moieties. It is possible that the mixture of tetrachloroplatinate and methotrexate may have formed polymer when mixed together in solution consistent with the similar findings found for the mixture and polymer. 

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