Cryopreservation, cells

The infusion of dimethylsulfoxide-cryopreserved autologous peripheral blood stem cells has been associated with a number of infusion-related adverse effects (4). Severe reversible encephalopathy was experienced by two patients after infusion of peripheral blood stem cells cryopreserved in 10% dimethylsulfoxide. In one patient, reduction of the plasma dimethylsulfoxide concentration by plasmapheresis resulted in marked improvement in encephalopathy (5). The presence of renal insufficiency may have predisposed to the development of neurological toxicity by delaying the excretion of dimethylsulfoxide and its metabolites. Other cases of dimethylsulfoxide-induced encephalopathy have been described (6-8). 

Primordial germ cells and spermatogonial stem cells cryopreservation... 

MAUGER PE, LE BAIL p Y and LABBfi c (2006) Cryobaukiug of fish somatic cells optimizations of fin explant culture and fin cell cryopreservation. Comp Biochem Physiol, 144,29-37. 

In a review of the published hterature, hundreds of adverse reactions, such as nausea, chills, cardiac dysrhythmias, neurological symptoms, and respiratory arrest, have been identified in association with the transplantation of stem cells cryopreserved with DMSO [28 ]. These adverse reactions are generally accepted as commonplace, as most of them are transient, although some patients may require treatment. 

Cox MA, Kastrup J, Hrubisko M. Historical perspectives and the future of adverse reactions associated with haemopoietic stem cells cryopreserved with dimethyl sulfoxide. Cell Tissue Bank 2012 13(2) 203-15. 

Trounson, A. Mohr, L. (1983). Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo. Nature 305, 707-710. 

The recent development and application of methodologies sensitive at the single cell wall level has shown that traditional bulk analytical techniques average out important intrinsic heterogeneity in sampled populations. By exploring the diversity of cell walls using novel cryopreservation techniques for electron microscopy and non-invasive... 

The shift to the use of PBPCs over bone marrow for autologous HCT is primarily because of the more rapid engraftment and decreased health care resource use. Because the harvest occurs before administering the preparative regimen, autologous hematopoietic cells must be cryopreserved and stored for future use. 

Pienta RJ, Poiley JA, Lebherz WB III. 1977. Morphological transformation of early-passage golden Syrian hamster embryo cells derived from cryopreserved primary cultures as a reliable in vitro bioassay for identifying diverse carcinogens. Int J Cancer 19 642-655. 

Chan S, Lam S, Leung V, Chan D, Luk K, Cheung K (2010) Minimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs. Eur Cells Mater 19 273-283... 

Finkelstein, M., et al., Immune function of cryopreserved avian peripheral white blood cells potential biomarkers of contaminant effects in wild birds, Arch. Environ. Contam. Toxicol., 44, 502, 2003. 

Finkle BJ, ZavolaME, Ulrich JM. Cryoprotective compounds in the viable freezing of plant tissues, in Cryopreservation of Plant Cells and Organs (Kartha KK, ed.), CRC Press, Boca Raton, FL, 1985, pp. 75-113. 

Kartha KK. Cryopreservation of Plant Cells and Organs, CRC Press, Boca Raton, FL, 1985. 

Takenouchi, T., Y. Iwamaru, M. Sato, T. Yokoyama, M. Shinagawa, and H. Kitani. 2007. Establishment and characterization of SV40 large T antigen-immortalized cell lines derived from fetal bovine brain tissues after prolonged cryopreservation. Cell Biolint 31(l) 57-64. 

Stem cell therapy involves infusion of specialized cells utilized to perform specific functions. The traditional use of cell therapy includes harvest and cryopreservation of autologous hematopoietic cells either from the bone marrow (old approach) or mobilization and pheresis of hematopoietic stem cells from peripheral blood using stem cell-mobilizing cytokines such as hematopoietic colony-stimulating factors (G-CSF, GM-CSF) or chemokine inhibitors (AMD-3100). A more recent stem cell source is umbilical cord blood that has rich pleuripotent potential and can engraft at lower doses than bone marrow or mobilized peripheral blood stem cells. 

At this time-point, the cells can be cryopreserved by suspending them in freezing medium to a concentration of 5 X 10 cells/mL, freezing them in isopropanol to -70°C for 24h, and then transferring them to a liquid nitrogen tank (-196°C). 

This paper covers our recent results devoted to cryopreservation of hemopoietic stem cells from cord blood and human fetal liver. 

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