L929 cells

To evaluate cell proliferation in the hydrogel, the L929 cells were immobilized at a density of 1.0 x 105 cells/mL. As a control sample, L929 cells were seeded onto a conventional cell culture plate at a density of 0.5 x 104 cells/mL. 

Fig. 7 Phase contrast microscope images of fibroblast (L929) cells on TCPS and in the PMBV/...

The PMBV/PVA hydrogel containing L929 cells was prepared and incubated for 7 days. After recovery from the PMBV/PVA hydrogel, L929 cells were fixed... 

Fig. 10 Cell cycle distribution of fibroblast (L929) cells depends on the elapsed time after encapsulated in the PMBV/ PVA hydrogel and on culture time on TCPS...

The ES cells were encapsulated in the PMBV/PVA hydrogel by the same method used for the L929 cells. In general, ES cells form a cell aggregate called an embryoid body in suspension culture. However, it was observed that the encapsulated ES cells in the hydrogel did not form any embryoid body for 72 h. 

Fig. 16 Fluorescence images of LIVE/DEAD assays of the L929 cells encapsulated for 4 days (a) in the miniaturized PMBV/PVA hydrogel formed in the microfluidic chip, and (b) in the bulk PMBV/PVA hydrogel formed in the 96-well microplate. Green fluorescence indicates live cells and red fluorescence indicates dead cells. Scale bar 100 pm...

Fig. 17 Viability of L929 cells after 4 and 8 days of encapsulation in miniaturized PMBV/PVA formed in a microfluidic chip and in bulk PMBV/PVA hydrogel formed in a 96-well microplate...

Cell cytotoxicity assays with the encapsulated L929 cells were performed. Methanol and CoCl2 solutions were used as toxins. Whereas methanol is a strong... 

Fisher, J. L. and Macdonald, R. L. (1997) Single channel properties of recombinant GABAa receptors containing y2 or 8 subtypes expressed with ai and p2 subtypes in mouse L929 cells../. Physiol. 505, 283-297. 

Bergmann, S., Shatrov, V., Ratter, F., Schiemann, S., Schulze-Osthoff, K., and Lehmann, V., 1994, Adenosine and homocysteine together enhance TNF-mediated cytotoxicity but do not alter activation of nuclear factor-icB in L929 cells,/. Immunol. 153 1736-1743. 

Ladner MB, Martin GA, Noble JA, Wittman VP, Warren MP, McGrogan M, Stanley ER, 1988. cDNA cloning and expression of minine macrophage colony stimulating factor from L929 cells. Proc Natl Sci USA 85 6706... 

Hauschildt, S., Scheipers, P., Bessler, W. G., and Mulsch, A. (1992). Induction of nitric oxide synthase in L929 cells by tumour-necrosis factor a is prevented by inhibitors of poly(ADP-ribose) polymerase. Biochem. J. 288, 255-260. 

L929 cells were exposed to osmolar stable solutions with variation of pH from 11.3 to 9.5 for 64 min. After this time, the medium was changed to normal MEM and the surviving cells were counted 24 h later. For pH over 10.75, a significant loss of cells can be detected after 1 h 24 h later, no regrowth occurs on these cells. 

Fig. 6.14 Exposure of L929 cells to osmolar stable solutions with variation of pH from 11.3 to 9.5 for 64 min...

Cel 1s and Vi rus. Encephalomyocarditls virus (EMC) and mouse L929 fibroblasts were obtained from Dr. Sidney Grossberg. L929 cells were routinely propagated in MEM containing 10% fetal bovine serum (Gibco). Fibroblast interferon-sensitive and resistant... 

The title substituted 6 phenylpurine bases and nucleosides 10—13, as well as some acetyl derivatives 8, were tested on their in vitro inhibition of the cell growth in the following cell cultures mouse leukemia L1210 cells (ATCC CCL 219) murine L929 cells (ATCC CCL 1) human cervix carcinoma HeLa S3 cells (ATCC CCL 2.2) and human T lymphoblastoid CCRF-CEM cell line (ATCC CCL 119). Only substituted 6-phenylpurine ribonucleosides 12 and their triacetates 8 exhibited significant activity in these assays (Table 1), while the bases 10 and 11, as well as the 2 amino 6 phenylpurine ribonucleosides 13, were entirely inactive. 

Murine L929 cells (ATCC CCL 1) were placed in 24-well tissue dishes and grown in Waymouth s MB 752/1 medium containing 5% fetal calf serum (10s cells/dish). After 24 h cell monolayers were overlaid with fresh medium supplemented with tested compounds at five different concentrations. After the additional 48-h incubation at 37 °C (C02 atmosphere) cultures were stained with methylene blue (Sigma)39 and the absorbance at 600 nm was then measured in 1% sarkosyl (Sigma) extracts. The number of cells was calculated from the calibration curve and results were expressed as IC50 values. 

---