Cell line mouse fibroblast

Ratnam, S. and Kent, C. (1995) Early increase in choline kinase-activity upon induction of the H-Ras oncogene in mouse fibroblast cell-lines. Archives of Biochemistry and Biophysics 323, 313—322. 

Much less is known about the cytotoxic and antiproliferative effects of the 20(S)-PPT family of ginsenosides. Ginsenoside Rhi has been reported to inhibit proliferation of the NIH 3T3 mouse fibroblast cell line but did... 

The phototoxicity test 3T3 NRU was proposed in 1994 and is so far the only in vitro method that has been validated by European regulatory authorities for predicting the photoirritant potential of substances [5,40,41]. In this test, the mouse fibroblasts cell line Balb/c 3T3 is exposed to simulated solar UV (or, more frequently, solar UVA) in the presence of the test compound after an incubation of 1 h in the dark. Evaluation of cytotoxicity is performed 24h post-exposure using the neutral red uptake (NRU) method. N RU permits to distinguish live and dead cells, since intact cells retain this dye (detailed method in INVITOX protocol 78). The validation was performed with substances selected on the basis of their in vivo photoirritant or phototoxic properties. Some of these structures are shown in Table 19.1. 

The tetraazatriphenylene chromophore attached to the cyclene ring acted as an efficient sensitiser for Eu3+ and Tb2+ emission but also intercalated between the base pairs of DNA. The complexes were tested as cellular imaging and reactive probes using the mouse fibroblast cell line. The complexes were quickly taken up by the fibroblast cells and localised in nucleus and around the cell membrane. The process was visualised by fluorescence microscopy. Photolysis at 340 nm and 350 nm damaged plasmid supercoiled DNA producing nicked (form II) and linear (form III) DNA. DNA damage is known to induce apoptotic cell death and these complexes may be therefore considered for the development as therapeutic probes, for example in the treatment of accessible tumours, such as skin melanoma. 

Before implantation several in vitro tests were performed. For evaluation of a possible toxic reaction, we investigated the material and the whole devices in vitro with cell culture methods. Direct contact and extraction tests with a mouse fibroblasts cell line (L 929) and a neuroblastoma cell line (neuro-2-a) were performed according to the international standard ISO 10993 ( Biological Evaluation of Medical Devices ). The materials and devices showed no toxicity, i.e. no significant differences in membrane integrity of the cell membranes, mitochondrial activity and DNA synthesis rate. The neuro-2-a cell line is so sensitive that even small changes in process technology are detectable. The flexible polyimide structures proved to be non toxic. 

Primary cells will continue or start to divide in culture but exhibit contact inhibition of movement (Abercrombie and Heaysman, 1954). When two such cells approach one another the characteristic ruffling movements of the cell membrane stop in the area of contact. Primary cells therefore do not grow one on top of the other and, in general, cease to divide when a monolayer has been formed. This phenomenon is not restricted to primary cells but applies also to many cell lines. An ideal example is the 3T3 mouse fibroblast cell line which grows rapidly in sparse culture but all division stops as soon as the cells become confluent at about 106 cells per 6 cm dish (Holley and Kieman, 1968). Such cells may for some time remain... 

Other immunogenicity in owl monkeys, in vitro cytotoxicity in mouse fibroblast cell lines, hemocompatibility, pyrogenicity, tissue implantation in rabbits... 

ECVAM Recommendation (2012) Recommendation concerning the cell transformation assays using Syrian hamster embryo cells (SHE) and the BALB/c 3T3 mouse fibroblast cell line for in vitro carcinogenicity testing... 

HA-PTX conjugates generated using this approach showed selective toxicity toward human cancer cell lines that are known to overexpress HA receptors while no toxicity was observed toward a mouse fibroblast cell line at the same concentration. Release of PTX from the conjugate occurred after cleavage of the labile 2 ester linkage. 

Jetten, A.M., Jetten, M.E.R., Shapiro, S.S. Poon, J.P. (1979a) Characterization of the Action of Retinoids on Mouse Fibroblast Cell Lines , Experimental Cell Research, 119, 289-99... 

Mouse Cells Liver and lung microsomes from C3H mouse fibroblast cell lines HPLC ... 

Wool and grain (wheat and barley) dusts stimulated TNF secretion by rat alveolar macrophages in vitro in a dose-dependent manner as measured in supernatants with the mouse fibroblast cell line L929 bioassay (Brown and Donaldson 1996). 

In lysates of the mouse fibroblast cell line, LMTK", the effects on NO at increasing RNA-binding activity were only observed when cells were made Fe-replete (Wardrop et al. 2000). Under these circumstances, iron regulatory protein 1 contains an [4Fe 4S] cluster that was susceptible to NO. In contrast, when lysates were prepared from cells treated with the Fe chelator desferrioxamine, NO... 

Aoki, J., M. Umeda, K. Takio, K. Titani, H. Utsumi, M. Sasaki, and K. Inoue. 1991. Neural cell adhesion molecule mediates contact-dependent inhibition of growth of near-diploid mouse fibroblast cell line m5S/lM. / Cell Biol 115 1751-1761. 

Protamine-based nanoparticles show a very low cytotoxicity in comparison with other gene delivery systems. In addition, when compared with commercially available liposomes (DOTAP, lipofectin), one artificial virus capsoid (polyoma VPl) and two cationic acrylate nanoparticles, the cell transfer efficiency in a mouse fibroblast cell line with protamine nanoparticles was one of the highest [117]. 

A permanent mouse fibroblast cell line, Balb/c 3T3, is maintained in culture for 24 h for the formation of monolayers. Two 96-well plates per test chemical are then pre-incubated with eight different concentrations of the chemical for 1 h. Thereafter one of the two plates is exposed to a non-cytotoxic UVAA is light dose of 5 I lor UVA (+UV experiment), whereas the other plate is kept in the dark (-UV experiment). In both plates, the treatment... 

In this study, biodegradable amphiphilic PCL-PEG-PCL copolymer was synthesized. In aqueous medium, this amphiphilic copolymer can form nano-sized micelles. The properties of nanoparticles as drag carriers were investigated, including CMC values and particle size. Moreover, the biocompatibility of polymeric nanopaiticles was also evaluated in vitro. The cytotoxicity was examined in L929 mouse fibroblasts cell line. Furthermore, nitric oxide (NO) production and reactive oxygen species (ROS> generation were also demonstrated. 

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