Interferon production

Each bottle for interferon production received thearborvirus preparation in medium 199 (0.5 ml) and further medium 199 (50 ml) some bottles received only medium 199 (50 ml) and no virus and served as controls. The bottles were incubated for 3 to 5 days at 36°C. 

The microencapsulation and controlled release of nucleic acids, e.g., poly(I C), for the stimulation of interferon production has been patented (87). 

Dosing and Administration Dose, frequency, and route of administration differ between the beta interferon products (see Table 26-3). 0 Dose-response curves have been observed with the beta interferons. However, it is not known if the total weekly dose or the frequency of administration is most important.37... 

McGovern, A. C. Ernill, R. Kara, B. V. Kell, D. B. Goodacre, R. Rapid analysis of the expression of heterologous proteins in Escherichia coli using pyrolysis mass spectrometry and Fourier transform infrared spectroscopy with chemometrics Application to a2- interferon production. J. Biotechnol. 1999, 72,157-167. 

Blakley BR, Archer DL, Osborne L. 1982. The effect of lead on immune and viral interferon production. Can J Comp Med 46 43-46. 

Aattouri N., Bouras M., Tome D., Marcos A. and Lemonnier D. (2001). Oral ingestion of lactic acid bacteria by rats increases lymphocyte proliferation and interferon -production . Br JNutr, 87, 367-373. 

Nickel salts have also been reported to have immunosuppressive properties in rodents, including giving acute thymic involution [389, 390], suppression of T lymphocyte response to T cell mitogens [389], suppression of antibody [389, 391, 392] and interferon production [393] and suppression of natural killer (NK) cell activity [389, 394-397]. 

McGovern et al.26 analyzed the expression of heterologous proteins in E. coli via pyrolysis mass spectrometry and FT-IR. The application was to a2-interferon production. To analyze the data, artificial neural networks (ANN) and PLS were utilized. Because cell pastes contain more mass than the supernatant, these were used for quantitative analyses. Both the MS and IR data were difficult to interpret, but the chemometrics used allowed researchers to gain some knowledge of the process. The authors show graphics indicating the ability to follow production via either technique. 

Interferon production of PBMCs cultured in the presence of selected DIMS determined by ELISA for IFNa, IFNp (mean data from six healthy individuals) and by Cytometric Bead Array (Becton Dickinson) for IFNy (mean data from four healthy individuals)... 

NTl 64 Sonnenfeld, G. Effect of sidestream tobacco smoke components on alpha/ beta interferon production. Oncology 1983 40(1) 52-56. 

Recent development highlighted a shift in FDA position and interpretation of the market exclusivity clause. Under the orphan designation, three interferon products, Betaseron (IFN-pib), Avonex (IFN-pia) and Rebif (IFN-pia) are currently approved for treatment of multiple sclerosis (MS). The interplay in drug safety and efficacy consideration, business strategies, and regulatory rationale permitting approval of the three drugs for MS treatment is discussed in Box 3.5. 

Cantell, K., S. Hirvonen, K.E. Mogensen, and L. Pyhala, Human leukocyte interferon production, purification, stability, and animal experiments. In vitro Monogr, 1974. 3 35-8. 

Rubinstein, M., S. Rubinstein, PC. Familletti, R.S. Miller, A.A. Waldman, and S. Pestka, Human leukocyte interferon production, purification to homogeneity, and initial characterization. Proc Natl Acad Sci USA, 1979.76(2) 640-4. 

The extent of CD8+ T cell activation can alternatively be assessed by quantitative evaluation of cytokine (e.g., 7-interferon) production and release measured by standard sandwich ELISA. The assay to be used for evaluating the extent of T cell activation needs to be selected and... 

The formation and release of interferon by viral and other pathological stimulation has resulted in a search for chemical inducers of endogenous interferon. Administration of a wide range of compounds has resulted in induction of interferon production. However, no clinically useful compounds have been found for humans, although tilorone is effective in inducing interferon in mice. 

In discussing the mechanism of antiviral protection and stimulation of interferon production in the mouse, DeClercq and Merigan46 concluded that there was a direct relationship between the extent of protection against vesicular stomatitis virus, the titers of interferon produced and the doses of tilorone. Giron et al.47, however, found no correlation between interferon induction and protection against MM virus in mice. Protection was achieved at doses far below the doses at which detectable interferon was found in the serum. Both findings may be consistent with differing mechanisms of viral inactivation for the two viruses under study. 

Other conditions previously mentioned in this chapter, such as cancer, immunocompetence and interferon production, antihistamine eflFects in colds, increased iron absorption, eflFects on cholesterol metabolism, and nitrosamine blocking, were studied at higher than RDA dosage levels. These many situations require additional research study to determine what the minimum levels are to attain the optimal health condition. 

Baker PN, Morser J Burke DC (1980) Effects of sodium butyrate on a human lymphoblastoid cell line (Namalwa) and its interferon production. Journal of Interferon Research 1 (1) 71-77. 

Robinson, B.W. and Rose, A.H. (1990). Pulmonary gamma interferon production in patients with fibrosing alveolitis. Thorax 45, 105-108. 

Mechanism of Interferon Production by Cells Exposed to Synthetic... 

It should be pointed out that the interferon inducers presented in Table 2 are widely different in activity, polyribonucleotide duplexes [such as poly(I) poly(C)] being superior to most other polynucleotides, especially those in which the 2 —OH group has been replaced by a 2 -H, 2 -F, 2 —Cl, 2 -N3, 2 -0-CH3 or 2 -0-CO-CH3 group (see Table 7). The interferon inducers listed in Table 2 also differ in the kinetics of interferon production poly-carboxylates (e.g. pyran copolymer, PAA, PMAA, COAM) and fluorenone... 

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