Becton Dickinson

Affinity Becton-Dickinson 20-30 coated cuvet color C... 

The submitters purchased L-cysteine hydrochloride monohydrate from Schwartz/Mann Division, Becton, Dickinson, and Company, Mountain View Avenue, Orangeburg, New York 10962. The checkers used material supplied by Aldrich Chemical Company, Inc. 

The full description of the deep chemical peel procedure is found in Chapter 8. Before the peeling, the subcision (subcutaneous incision) technique is used to free the fibrous bands from the base of the scars. For this purpose we use an i8-gauge 1.5-inch NoKor Admix needle (Becton Dickinson and Co). This needle has a triangular tip similar to No. 11 blade (Fig. 9.5). it allows smooth separation of fibrous cords. The needle is inserted through a skin surface, and its sharp edges are maneuvered under the defect to make subcutaneous cuts or incisions. The depression... 

Centrifuge tubes Becton-Dickinson BlueMax polypropylene, 15-mL SPE column adapters for 1-, 3- and 6-mL Bond Elut columns SPE plastic reservoirs 75-mL Disposable flow control valve liners for SPE... 

Recent innovations for detecting malaria include DNA or RNA probes by polymerase chain reaction (PCR). These, however, are not widely available for clinical use. A rapid dipstick test (ParaSight F, Becton-Dickinson, Cockeyville, MD) reportedly has a sensitivity of 88% and a specificity of 97%, which is comparable to microscopy. However, ParaSight F can give false-positive results with rheumatoid factor thus microscopy remains the optimal test. 

Smiths Detection DoE Oak Ridge National Lab Johns Hopkins University APL UTC Hamilton-Sundstrand IGEN International General Dynamics Becton-Dickinson... 

If visible tissue debris is found in the microtube, a 23G1 Precision Glide Needle (Becton-Dickinson, Franklin Lakes, NJ) with a lmL syringe is used to break down the cells through suctioning, until the solution becomes of clear appearance. This procedure may take 15-30 min. 

Corning Drierite Fisher-Johns Luer-Lok Corning Glass Works, Corning, New York W. A. Hammond Drierite Company, Xenia, Ohio Fisher Scientific Company, Pittsburgh, Pennsylvania Becton, Dickinson and Company, Rutherford, New Jersey... 

T. Schulte, personal communication, Becton Dickinson Diagnostic Instrument Systems, Towson, Maryland 1989. 

Support of this work by Becton Dickinson Advanced Diagnostics is gratefully acknowledged. For insightful suggestions regarding this work, the author thanks Robert W. Rosenstein, Lewis Pollack, Gary Krauth, and Joseph R. Lakowicz. 

Lab-Lemco (Oxoid) (42 g) peptone from casein (52.5 g) yeast extract (52.5 g) casitone (Becton Dickinson) (31.5 g)... 

L-shaped spreaders, plastic, sterile (e.g. VWR1612-1560, VWR International) two polypropylene tube, 50 mL, screw-capped, presterilized (e.g. Falcon tubes, Becton Dickinson Labware, Franklin Lakes, NJ, USA)... 

Variations for 5-hydroxy fluvastatin. Medium for growth on agar plates Plate Count Agar (Fluka/ Sigma Aldrich, Buchs, Switzerland) medium for preculture and main culture glucose 20 g L , soytone (Becton Dickinson) 15 g L , yeast extract 10 g L , pH adjusted to 6.5 with NaOH. Incubation time of main culture before fluvastatin addition 3 days biotransformation period 24 h. 

Fig. 1. Photomicrographs of a reactive lymph node follicle with germinal center (G), mantle (M), and surrounding paracortex (P) immunostained using the avi-din-biotin complex technique. (Top) Follicle stained with an antibody specific to B-cells, B1 (CD20) (Coulter Immunology, Hialeah, FL), counterstained with Methyl Green. (Bottom) Parallel section of the same follicle stained with an antibody specific to T-cells, Leu 4 (CDS) (Becton-Dickinson, Mountain View, CA). Scale bar = 100 p.

There are cnrrently two sources of monoclonal antibodies to bromodeoxynridine Becton Dickinson Immunocytochemistry Systems and Boehringer Mannheim Biochemicals. Both antibodies have worked eqnally well for the anthor, and both are directed against single-stranded DNA, therefore necessitating the removal of DNA-associated histones and denaturation of the donble-stranded DNA. 

Sterile water for injection, USP (Abbott Laboratories, North Chicago, IL). Polypropylene, 50-mL sterile, conical centrifuge tubes (Becton Dickinson Labware, Lincoln Park, NJ). 

Polypropylene, 5.5-mL sterile tubes (Becton Dickinson Labware). 

Figure 6.2 Cell cycle distributions of MCF-7 cells after treatment with TSA for 24 hours were determined by performing flow cytometric analysis. Following treatment of cells with 50 nM and 1 P-M of TSA, the samples were analyzed on a Becton-Dickinson FACStarP "" instrument and the percentage of nuclei with C], S, and C2/M DNA content was determined. p21 protein was also correspondingly increased at various intervals.

Human IFNy Cytometric Bead Array (CBA) Flex set (Becton Dickinson, CA, USA). 

The presence of IFNy in cell culture supernatants from PBMCs stimulated with DIMS compounds was detected using CBA flex kit (Becton Dickinson) on a FACSArray flow cytometer (Becton Dickinson). The data were analyzed using FCAP Array software (Becton Dickinson). 

Interferon production of PBMCs cultured in the presence of selected DIMS determined by ELISA for IFNa, IFNp (mean data from six healthy individuals) and by Cytometric Bead Array (Becton Dickinson) for IFNy (mean data from four healthy individuals)... 

Excitation and emission spectra of molecules for donor-acceptor pairs can be found at one of the following Web sites Becton-Dickinson Fluorescence Spectrum Viewer (http //www. bdbiosciences.com/spectra), Invitrogen-Molecular Probes Fluorescence Spectra Viewer (http //www.probes.invitrogen. com/servlets/spectraviewer). 

Matrigel (growth factor reduced), Becton Dickinson. 

Fig. 9. Flow analysis of apoptotic human peripheral blood lymphocytes using direct TUNEL assay. Human peripheral blood lymphocytes (1 x 10 ) treated (A) without or (B) with dexamethasone (0.1 ijlM) for 16 h were transferred to a 15-ml tube. Paraformaldehyde (2%) was added to cells with shaking and incubated for 10-15 min in ice with occasional shaking. Cells were washed with PBS with 1% BSA and 3-4 ml cold acetone was then added to cells. After 2-3 min incubation on ice with occasional shaking, cells were washed twice and TUNEL reaction mixture including enzyme TdT and fluorescein-labeled anti-dUTP antibody was added to cells (H). For the negative control group, only label solution without TdT was added to cells ( ). Cells without any addition of reaction or label solution were used for assessment of the autofluorescence ( ). The cell mixture was incubated 1 h at 37°C in the dark. The result of the apoptosis after flow analysis was expressed as a histogram using software CellQuest (Becton Dickinson). In Fig. 9A Ml (nonapoptotic cells), 86% M2 (apoptotic cells), 14%. In Fig. 9B Ml (nonapoptotic cells), 78% M2 (apoptotic cells), 22% (our unpublished data).

Fig. 10. DNA fluorescence flow cytometric profiles of Pl-stained lymphocytes after 12 h incubation with 1 jiM dexamethasone (Zl). Cells were fixed with 0.25% paraformaldehyde and treated with 70% ethanol at 4 C for 1 h to increase the cell membrane permeability. PI (50 fig/mV) was used to stain DNA for 30 min. Results of flow cytometer (FACSCAN, Becton Dickinson, California) were expressed as a histogram. Dexamethasone-treated lymphocytes showed a small peak before the Gq/Gi peak of diploid cells. This characteristic sub-Go peak represents the DNA in apoptotic cells.

X TTliquid medium. 1% Yeast extract, 1.6% Pepton (Becton, Dickinson and Company, cat. No. 211677), 0.5% NaCl. Adjust pH to 7.0 with 2N NaOH. Premix powder is also commercially available (e.g., Becton, Dickinson and Company, cat. No. 244020). Sterilize by autoclaving. Ampicillin or kanamycin should be added at 50 pg/ml if needed. 

Flexible plate, 96 well, U-bottom without lid (BECTON DICKINSON, cat. No. 353911). There are several 96-well plates, but this product is easily handled especially for restriction enzyme digestion of a large number of DNA samples. 

Becton-Dickinson Labware (Falcon) 1950 Willaim Drive Oxnard, CA 93030 (USA)... 

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