Human umbilical vein endothelial

Human umbilical vein endothelial cells (HUVEC) express the isoforms ECE-la, -lb, -Id and ECE-2. In these cells, ET-1 is secreted via both a constitutive and a regulated pathway. The ratio of released ET-1 big-ET-1 is 4 1. About 80% of the ET-1 is secreted at the abluminal cell surface of endothelial cells. ECE-isoforms are abundantly expressed on the cell surface of endothelial cells and to a lower level also on vascular smooth muscle cells. In atherosclerotic lesions of vessels, however, ECE expression in smooth muscle cells is upregulated. ECE isoforms expressed in smooth muscle cells contribute significantly to the generation of mature ET in normal and in particular atherosclerotic vessels. 

Expression (Human) Tissues Leukocytes, thymus, spleen, liver, ovary Cells PBLs, neutrophils,T-cells, dendritic cells, mast cells, eosinophils, macrophages, leukocytes Tissues spleen, small intestine, placenta, lung smooth muscle, Cells bronchial smooth muscle, CD34+ hemapoietic progenitor cells, monocytes, macrophages, mast cells, eosinophils, neutrophils, PBLs, human umbilical vein endothelial cells Tissues, heart, skeletal muscle, spleen, brain, lymp node, adrenal medulla, lung, human pumonary/ saphenous vein Cells monocytes, macrophages, mast cells, eosinophils, cardiac muscle, coronary artery, PBLs... 

The adherence and proliferation of human umbilical vein endothelial cells (HUVECs) were evaluated on the fabricated PCLA scaffold. Results showed that the HUVECs were adhered and proliferated well on the small-diameter-fiber fabrics (0.3 and 1.2 mm in diameter), whereas markedly reduced cell adhesion, restricted cell spreading, and no signs of proliferation were observed on the large-diameter-fiber fabric (7 mm in diameter). That may be due to the high-surface-density fibers provide an extremely high surface/volume ratio, which favors cell attachment and proliferation. 

Schmaier AH, Kuo A, Lundberg D, Murray S, Cines DB The expression of high molecular weight kininogen on human umbilical vein endothelial cells. J Biol Chem 1988 263 16327-16333. 

Kaneko et al. (1993) have described a group of lipophilic ascorbic-acid analogues that have been studied in cultured human umbilical vein endothelial cells that were first incubated with test drug and then exposed to lipid hydroperoxides. Although ascorbate itself did not protect the endothelial cells, derivatives like CV3611 protected. Pretreatment was necessary. CV3611 was synergistic with vitamin E. The authors concluded that these lipophilic antioxidants incorporate into endothelial cell membranes where they are effective inhibitors of lipid peroxidation. In contrast, lipophobic antioxidants were not effective in their hands (Kaneko et al., 1993). 

HUVEC Human umbilical vein endothelial cell... 

Gentilini G, Kirschbaum NE, Augustine JA, Aster RH, Visentin GP. Inhibition of human umbilical vein endothelial cell proliferation by the CXC chemokine, platelet factor 4 (PF4), is associated with impaired downregulation of p21(CiplAVAFl). Blood 1999 93(1) 25—33. 

MDCK (Madin-Darby canine kidney) cells are derived from distal tubules, whereas LLC-PKi are from proximal tubes. b BMEC (brain microvessel endothelial cells) are isolated from capillaries. BPAEC (bovine pulmonary artery endothelial cells), BAEC (bovine aortic endothelial cells), and HUVEC (human umbilical vein endothelial cells) are large vessel endothelia. 

Flahaut, E. et al. (2006) Investigation of the cytotoxicity of CCVD carbon nanotubes towards human umbilical vein endothelial cells. Carbon, 44 (6), 1093-1099. 

Schweitzer KM, Vicart P, Delouis C, Paulin D, Drager AM, Langenhuijsen MM, Weksler BB. Characterization of a newly established human bone marrow endothelial cell line distinct adhesive properties for hematopoietic progenitors compared with human umbilical vein endothelial cells. Lab Invest 1997 76 25-36. 

TIRFM was used for time-lapse observations of initial cell adhesion to SAMs with different surface functionalities (Fig. 2). After 10 min of plating a suspension of human umbilical vein endothelial cells (HUVECs), a few bright spots were observed on SAMs with COOH and NH2 functionalities this indicated cell adherence. The number of bright spots increased and the spot areas enlarged with incubation time, indicating that HUVECs adhered and spread well on COOH-SAM and NH2-SAM surfaces. Quantitative analysis of the number of adherent cells and cell adhesion areas... 

A. Pailleret, J. Oni, S. Reiter, S. Isik, M. Etienne, F. Bedioui, and W. Schuhmann, In situ formation and scanning electrochemical microscopy assisted positioning of NO-sensors above human umbilical vein endothelial cells for the detection of nitric oxide release. Electrochem. Commun. 5, 847-852 (2003). 

HUVEC, human umbilical-vein endothelial cells... 

Fig. 9.2. Physiological concentrations of estradiol decrease the production of F2a-isoprostanes in medium from cultured human umbilical vein endothelial cells in culture. (From Hermenegildo et al. 2002)...

Fig. 12. Inhibition of l25I-lys-plasminogen binding to human umbilical vein endothelial cells (HUVECs) by Lp(a) and apo(a). Confluent HUVEC monolayers were washed, treated with -aminocaproic acid, rewashed and incubated with 125I-lys-plasminogen (4.95 nM, specific activity 415.000cpm-pmo -1), for 30 min at 4°C in the presence of various excess amounts of unlabeled Lys-PLG (A) Lp(a) (0,0) apo(a) (x) LDL ( , ) or Lp(-) (V). [With permission of Hajjar el at. (HI 1).]...

Cho MM, Ziats NP, Pal D, Utian WH, and Gorodeski GI [1999] Estrogen modulates paracellular permeability of human umbilical vein endothelial cells by eNOS and iNOS related mechanisms. Am J Physiol 276 C337-C349... 

Sphingosine 1 -phosphate protects human umbilical vein endothelial cells from semm-depiived apoptosis by nitric oxide production, J. Biol. Chem. 276 10627-10633. 

Sjostrom, M., Johansson, A. S., Schroder, O., Qiu, H., Palmblad, J., and Haeggstrom, J. Z. (2003) Dominant expression of the CysLT(2) receptor accounts for calcium signaling by cysteinyl leukotrienes in human umbilical vein endothelial cells. Arterioscler. Thromb. Vase. Biol. 23, E37-E41. 

Human umbilical vein endothelial cells (HUVEC), used to exemplify tumor spheroid-based migration on a cell monolayer. 

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