Human umbilical vein endothelial cells HUVECs

Human umbilical vein endothelial cells (HUVEC) express the isoforms ECE-la, -lb, -Id and ECE-2. In these cells, ET-1 is secreted via both a constitutive and a regulated pathway. The ratio of released ET-1 big-ET-1 is 4 1. About 80% of the ET-1 is secreted at the abluminal cell surface of endothelial cells. ECE-isoforms are abundantly expressed on the cell surface of endothelial cells and to a lower level also on vascular smooth muscle cells. In atherosclerotic lesions of vessels, however, ECE expression in smooth muscle cells is upregulated. ECE isoforms expressed in smooth muscle cells contribute significantly to the generation of mature ET in normal and in particular atherosclerotic vessels. 

The adherence and proliferation of human umbilical vein endothelial cells (HUVECs) were evaluated on the fabricated PCLA scaffold. Results showed that the HUVECs were adhered and proliferated well on the small-diameter-fiber fabrics (0.3 and 1.2 mm in diameter), whereas markedly reduced cell adhesion, restricted cell spreading, and no signs of proliferation were observed on the large-diameter-fiber fabric (7 mm in diameter). That may be due to the high-surface-density fibers provide an extremely high surface/volume ratio, which favors cell attachment and proliferation. 

TIRFM was used for time-lapse observations of initial cell adhesion to SAMs with different surface functionalities (Fig. 2). After 10 min of plating a suspension of human umbilical vein endothelial cells (HUVECs), a few bright spots were observed on SAMs with COOH and NH2 functionalities this indicated cell adherence. The number of bright spots increased and the spot areas enlarged with incubation time, indicating that HUVECs adhered and spread well on COOH-SAM and NH2-SAM surfaces. Quantitative analysis of the number of adherent cells and cell adhesion areas... 

Fig. 12. Inhibition of l25I-lys-plasminogen binding to human umbilical vein endothelial cells (HUVECs) by Lp(a) and apo(a). Confluent HUVEC monolayers were washed, treated with -aminocaproic acid, rewashed and incubated with 125I-lys-plasminogen (4.95 nM, specific activity 415.000cpm-pmo -1), for 30 min at 4°C in the presence of various excess amounts of unlabeled Lys-PLG (A) Lp(a) (0,0) apo(a) (x) LDL ( , ) or Lp(-) (V). [With permission of Hajjar el at. (HI 1).]...

Human umbilical vein endothelial cells (HUVEC), used to exemplify tumor spheroid-based migration on a cell monolayer. 

In a more recent paper [160], Urry et al. reported that x20-poly (GGAP) was nonadhesive to both bovine ligamentum nuchae fibroblast and human umbilical vein endothelial cells (HUVECs), even in the presence of serum. Urry et al. interpreted this behavior in terms of their hydrophobicity scale. They suggested that x20-poly[50(GGAP), (GRGDSP)], for example, could provide the inner lamina for a vascular prosthesis. 

Figure 6.2 Immunocytochemical detection of catechol-O-methyl transferase (COMT) in human umbilical vein endothelial cells (HUVEC). Polyclonal COMT antibody from Chemicon Millipore, USA. (From Kravets E. [2008].)...

HSMCs) and human umbilical vein endothelial cells (HUVECs) were microinjected in the nucleus (top) and cytoplasm with CMV-driven, GFP-expressing plasmids containing either... 

Human Umbilical Vein Endothelial Cells (HUVECs)... 

Human umbilical vein endothelial cells (HUVEC) were trypsinized, then washed in DMEM, 10% FBS plus antibiotics, and centrifuged. The cells were then resuspended in DMEM and 10% FBS and antibiotics to achieve a concentration of 3 x 105 cells/ml. Thereafter the cells were diluted to 3 x 104 cells/ml in DMEM and 10% FBS plus antibiotics and incubated 22 hours at 37°C. 

The development of parallel-plate perfusion chambers [67,68] made possible the study of platelet interaction with the extracellular matrix (ECM) generated by cells in culture or with isolated subendothelial components under defined experimental conditions. The use of the ECM produced by human umbilical vein endothelial cells (HUVEC) in culture as adhesive substrate has Su tated the understanding of the mechanisms involved in primary hemostasis [68]. HUVECs are immature and not subjected to flow conditions during their culture, two Ikctors which may influence the reactivity of their ECM towards platelets [69]. Interestingly, the properties and reactivity of the underlying ECM can be modified by exposure of HUVECs to different stimuli, an experimental approach which has fevored the investigation of basic mechanisms of thrombosis [33]. 

Garcia-Cardena reported in 1996 that the tyrosine phosphorylation of eNOS in bovine aortic endothelial cells (BAEC) resulted in a decrease in enzyme activity whilst immunoprecipitation of caveolin-1, the predominant isoform in endothelial cells, resulted in co-immunoprecipitation of tyrosine phosphorylated eNOS [17]. Michel et al. (1997) further reported that eNOS, also in BAEC, was co-immunoprecipitaed by caveolin-1 antibodies whilst in rat myocytes eNOS was associated with caveolin-3, the cardiac muscle specific type, confirming that eNOS interaction with caveolin is not tissue specific [18]. Immunofluoresence studies in our group have shown that eNOS associates with caveolin-1 in human umbilical vein endothelial cells (HUVEC), and this interaction is abolished by bradykinin (Wyatt, Pedley Mann, 2001 unpublished data) (Figure 3). 

It has been shown that combretastatin A-4 disrupts the microtubules of human umbilical vein endothelial cells (HUVECs) in culture, thus confirming that the tubulin binding properties shown in cell-free systems are retained when the compound enters cells and that tubulin binding is a significant component of the biological activity. Also 3-fluoro- and 3-chloro derivatives retained activity in human umbilical vein endothelial cells. This kind of activity against endothelial cells is extremely important, as endothelial cells play a key role in the angiogenic process. 

Cells COS-7 cells (the African green monkey kidney fibroblast cell line), S-180 cells (mouse sarcoma), Meth-A fibrosarcoma cells (mouse fibrosarcoma), Jurkat cells (human T cell line). Colon 26 cells (mouse colon adenocarcinoma), B16BL6 cells (mouse meranoma). Human umbilical vein endothelial cells (HUVEC) (Kurabo Industries, Osaka, Japan). 

---