Human stem cells

Costa JJ, Demetri GD, Harris TJ. Dvorak AM. Hayes DF, Merica EA, Menchaca DM. Gringeri AJ. Schwartz LB, Galli SJ Recombinant human stem cell factor (kit ligand) promotes human mast cell and melanocyte hyperplasia and functional activation in vivo. J Exp Med 1996 183 2681-2686. 

Ponomaryov T, Peled A, Petit I, et al. Induction of the chemokine stromal-derived factor-1 following DNA damage improves human stem cell function. J Clin Invest 2000 106(11) 1331—1339. 

Dick JE, Guenechea G, Gan 01, Dorrell C. In vivo dynamics of human stem cell repopulation iuNOD/SGID rrrice. Ann N Y Acad Sci. 2001 938 184-190. 

Guenechea G, Gan Of, Dorrell C, Dick JE. Distinct classes of human stem cells that differ in proliferative and self renewal potential. Nat Immunol. 2001 2 75-82. 

Mazurier F, Doedens M, Gan 01, Dick JE. Rapid myeloeiythroid repopulation after intrafemoral transplantation of NOD-SCID mice reveals a new class of human stem cells. Nat Med. 2003 9 959-963. 

ADC- ASSAY WITH HUMAN STEM CELL IN VITRO... 

Incorporation of other differentiation end points like neural and osteoblast differentiation and the use of human stem cells can lead to more sophisticated and defined end points. 

Compare the responses of human stem cells versus murine stem cells and explore mechanisms of conflict resolution when these two disagree (e.g., a comparison between the effects of xenobiotics in mESC and hESC might be useful). 

In the last decade stem cell research has justifiably gained one of the highest scientific profiles both within the medical community and the general public. The ability of stem cells to undergo both self-renewal and differentiation into more functionally specialised mature cells offers tremendous promise in TE. The isolation, expansion and directed differentiation of human stem cells in vitro, however, demand improved understanding of the interplay between the... 

Brady RP, Newberry MS, Girard VW. The Food and Drug Administration s Statutory and Regulatory Authority to Regulate Human Pluripotent Stem Cells. In Ethical Issues in Human Stem Cell Research, vol. 1. Report and Recommendations of the National Bioethics Advisory Commission, Appendix D, pp. 93-8. 

Regarding human stem cell therapy, scientists are developing a number of strategies for... 

Perhaps the application for human stem cells with the most potential is the generation of cells and tissues for the cell-based therapies. The demand for donated organs and tissues far outweighs the supply. Stem cells could possibly be directed toward the production of replacement cells and tissues to treat Parkinson s disease, Alzheimer s disease, spinal cord injury, stroke, burns, heart disease, diabetes, osteoarthritis, and rheumatoid arthritis. 

Bieberich E, Silva J, Wang G, Krishnamurthy K, Condie BG. Selective apoptosis of pluripotent mouse and human stem cells by novel ceramide analogues prevents teratoma formation and enriches for neural precursors in ES cell-derived neural transplants. J. Cell Biol. 2004 167 723-734. 

Human embryonic stem cells were first collected in 1998 by two different research teams. The cells obtained from the inner cell mass of the blastocyst (4- to 5-day embryo) are embryonic stem cells (ESC) in contrast, cells cultured from the primordial germ cells of 5- to 9-week fetuses are embryonic germ cells (EGC). In the laboratory, ES or EG cells can proliferate indefinitely in an undifferentiated state but can also be manipulated to become specialized or partially specialized cell types, a process known as directed differentiation. Both ES and EG cells are pluripotent, meaning they have the potential to develop into more than 200 different known cell types. This class of human stem cells holds the promise of being able to repair or replace cells or tissues that are damaged or destroyed by many of our most devastating diseases and disabilities. 

DISULFIDE-LINKED HUMAN STEM CELL FACTOR DIMER... 

Disulfide-Linked Human Stem Cell Factor Dimer... 

Irani, A.A., Nilsson, G., Miettinen, U., Craig, S.S., Ashman, L.K., Ishizaka, T., Zsebo, K.M. and Schwartz, L.B. (1992). Recombinant human stem cell factor stimulates differentiation of mast cells firom dispersed human fetal fiver cells. Blood 80, 3009-3021. 

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