Human promyelocytic leukemia cell line

Nakatani N, Ichimaru M, Moriyasu M, Kato A. Induction of apoptosis in human promyelocytic leukemia cell line HL-60 by C-benzylated dihydrochalcones, uvaretin, isouvaretin and diuvaretin. Biol Pharm Bull 2005 28 83-86. 

Nauseef, W. M. (1986). Myeloperoxidase biosynthesis by a human promyelocytic leukemia cell line Insight into myeloperoxidase deficiency. Blood 67,865-72. 

Mangelsdorf, D.J. et al. (1984) 1,25-Dihydroxyvitamin D3-induced differentiation in a human promyelocytic leukemia cell line (HL-60) receptor-mediated maturation to macrophage-like cells. The Journal of Cell Biology, 98, 391-398. 

Methyl-1,3-dihydropyrrolo[ 1,2-c]thiazole-6,7-biscarbamates have been described as active compounds against murine P388 lymphocytic leukemia <87JMC2109>. Several 5-aryl-2,3-dihydro-pyrrolo[2,l-fe]thiazole-6,7-dimethanol-6,7-bis(isopropylcarbamate)s were tested for growth inhibitory activity with the HL-60 human promyelocytic leukemia cell line. The 5-phenyl, 5-(4-fluorophenyl), and 5-(3,4-dichlorophenyl) have antileukemic activity. These compounds were also cytotoxic to HT-29 human colon carcinoma cells <88JMC1427>. 

HL60 cells (a human promyelocytic leukemia cell line). 

S. J. (1980) Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid. Proc. Natl. Acad. Sci. USA 77,2936-2940. 

Shi L et al (1996) Granzyme B induces apoptosis and cyclin A-associated cyclin-dependent kinase activity in all stages of the cell cycle. J Immunol 157 2381-2385 Shimizu T et al (1995) Unscheduled activation of cyclin Bl/ Cdc2 kinase in human promyelocytic leukemia cell line HL60 cells undergoing apoptosis induced by DNA damage. Cancer Res 55 228-231... 

Alcam, F. J., Huron, M. I., Rodriguez-Aguilera, J. C., Villalba, J. M., and Navas, P., 1990, Ascorbate free radical stimulates the growth of a human promyelocytic leukemia cell line. Cancer Res. 50 5887-5891. 

Benedict et al. (1980) found that chemically transformed cells in culture could be induced by ascorbic acid to revert to their original morphologic status. Ascorbic acid was also found to inhibit the chemically induced morphologic transformation of cells. Alcain and associates (1990) found that the growth of a human promyelocyte leukemia cell line could be stimulated by the ascorbate free radical. Tauchi and Sawada (1993), on the other hand, found that ascorbic acid could suppress... 

Cells were cultured in a humidified ahnospheric incubator at 37°C in 5% CO2. Human promyelocytic leukemia cell line (HL-60) was cultured in RPMI 1640 supplemented with 13% heat-inactivated fetal bovine serum and SO U/mL penicillin-streptomycin. To induced myeloid differentiation, cells were seeded at a density of S IO cells/mL and were cultivated for five days in RPMI 1640 containing 1.24% DMSO. TTie characteristics of mature cells were determined by smaller cell size, decreased nucleoli-to-cytoplasm ratio and pyknotic changes in nuclear chromatin. Cell numbers were counted using a hemocytometer, and cell viability was >98% as evidenced by trypan blue staining. 

Hashimoto, Y, Petkovich, M, Gaub, M. P., Kagechika, H, Shudo, K, and Chambon, P. (1989) The retinoic acid receptors ot and P are expressed in the human promyelocytic leukemia cell line HL-60 Mol. Endocrinol 3,1046-1052. 

As for medical applications two types of succinoyl trehalose lipids produced by R. erythropolis SD-74 were found to induce cell differentiation instead of cell proliferation in the human promyelocytic leukemia cell line HL60. Both compounds induced HL60 to differentiate into monocytes. Furthermore, they also inhibited protein kinase C (PKC) activities of HL60 cells indicating potential for antitumoral agents [33], Other investigations showed that... 

Inoue S, Itoh S (1982) Sophorolipids from Torulopsis bombicola as microbial surfactants in alkane fermentation. Biotechnol Lett 4 308-312 Isoda H, Shinmoto H, Kitamoto D, Matsumura M, Nakahara T (1997) Differentiation of human promyelocytic leukemia cell line HL60 by microbial extracellular glycolipids. Lipids 32 263-271... 

Isoda, H., Kitamoto, D., Shinmoto, H., Matsumura, M.,Nakahara, T. Microbial extracellular glycolipid induction of differentiation and inhibition of protein kinase C activity of human promyelocytic leukemia cell line HL60. Biosci Biotechnol Biochem. 1997,61, 609-614. 

Nojiri, H., Takaku, F., Ohta, M., Miura, Y, and Saito, M., 1985, Changes in glycosphingolipid composition during differentiation of human leukemic granulocytes in chronic myelogenous leukemia compared with in vitro granulocytic differentiation of human promyelocytic leukemia cell line Hl 60, Cancer Res. 45 6100-6106. 

Saito, M., Terui, Y., and Nojiri, H., 1985, An acidic glycosphingolipid, monosialoganglioside GM3 is a potent physiological inducer for monocytic differentiation of human promyelocytic leukemia cell line HL-60 cells, Biochem. Biophys. Res. Commun. 132 223-231. 

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