HL-60 promyelocytic leukemia cells

An extract from Lactuca indica showed significant free radical scavenging activity, and protected phixl74 supercoiled DNA against strand cleavage and reduced oxidative stress in human promyelocytic leukemia HL-60 cells. On account of protocatechulic acid, methyl p-hydroxybenzoate, caffeic acid, 3,5-dicaffeoylquinic acid, luteolin 7-O-fT glucopyranoside, and quercetin 3-0-(3-g 1 ucopyranoside are the major antioxidative constituents (111). 

Liu, Y., Chang, R.L., Cui, X.X., Newmark, H.L. and Conney, A.H. 1997. Synergistic effects of curcumin on all-trans retinoic acid- and 1 alpha,25-dihydroxyvitamin D3-induced differentiation in human promyelocytic leukemia HL-60 cells. Oncol Res 9 19-29. 

Classic antioxidants, vitamin E, vitamin C, and others can suppress the activation of apoptosis. For example, ascorbic acid prevented cytochrome c release and caspase activation in human leukemia cells exposed to hydrogen peroxide [128], Pretreatment with A -acctylcystcinc, ascorbate, and vitamin E decreased homocysteine thiolactone-induced apoptosis in human promyelocytic leukemia HL-60 cells [129]. Resveratrol protected rat brain mitochondria from anoxia-reoxygenation damage by the inhibition of cytochrome c release and the reduction of superoxide production [130]. However, it should be mentioned that the proapoptotic effect of ascorbate, gallic acid, or epigallocatechin gallate has been shown in the same human promyelocytic leukemia cells [131]. 

As discussed in the previous sections, a number of triterpenoids with great structural diversity have antitumor-promoting activities. In contrast, 28-deacetylbelamcandal (288), a spiroiridal-type triterpenoid, has recently been reported to possess tumor promoting activity [92]. Compound 288, which stimulated differentiation of human promyelocytic leukemia (HL-60) cells (a fast method for screening TPA-type tumor promoters),... 

The polyphenol extract from the barley bran (BPE) induced the differentiation of human promyelocytic leukemia HL-60 cells, based on the expression of the differentiation markers such as nitro blue tetrazolium (NBT) reducing activity and a-naphthyl butyrate esterase activity. Prodelphini-din B-3, Tl, T2, and T3 induced 26-40% NBT-positive cells and 22-32% a-naphthyl butyrate esterase-positive cells. Proanthocyanidins might potentiate the retinoic acid (all-frarcs-retinoic acid)-induced granulocytic differentiation and sodium butyrate-induced monocytic differentiation in the HL-60 cells [101]. 

Enzyme activity was assayed in the high speed supernate obtained from rat livers homogenized in 50 mM potassium phosphate buffer (pH 7.4) containing 0.1 M NaCI. It was also shown to be applicable to extracts from human promyelocytic leukemia HL-60 cells, and mouse embryo fibroblasts. 

Stevens, R.L., Avraham, S., Gartner, M.C., Bruns, G.A., Austen, K.F. and Weis, J.H. (1988a). Isolation and characterization of a cDNA which encodes the peptide core of the secretory granule proteoglycan of human promyelocytic leukemia HL-60 cells. J. Biol. Chem. 263, 7287-7291. 

M. Control of IL-2 receptor-alpha expression by lL-1, tumor necrosis factor, and lL-2. Complex regulation via elements in the 5 flanking region. J. Immunol. 145, 3340-3347 (1990). Plaetinck, G., Van der Heyden, J., Tavernier, J., Fache, I., Tuypens, T., Fischkoff, S., Fiers, W., and Devos, R. Characterization of interleukin-5 receptors on eosinophilic sublines from human promyelocytic leukemia (HL-60) cells. J. Exp. Med. 172, 683-691 (1990). 

A new cholestane glycoside (18) with a benzoyl-rhamnoglucosyl moiety isolated from Ornithogalum saundersiae strongly inhibited in vitro the growth of human promyelocytic leukemia HL-60 cells and human T-lymphocytes leukemia MOLT-4 cells with IC50 of 21 and 18 nM... 

Recently, Kinghorn et al. [96] reviewed the anticancer agents discovered by activity-guided fractionation and they include seventy compounds from twenty-two plants. Only one lignan is cited in the review guaiacylglycerol-P-06 -(2-methoxy)cinnamyl alcohol ether isolated from Brucea javanica (Simaroubaceae) exhibited weak activity against the induction of cell differentiation of human promyelocytic leukemia (HL-60) cells [96,97],... 

As part of a search for chemopreventive agents from natural products, nearly 400 plant extracts were tested for their potential to induce human promyelocytic leukemia (HL-60) cell differentiation. Seventeen of the plant extracts were active, with ED50 values of 4 pg/mL [139], One of most potent was an extract derived from Dirca occidentalis (Thymelaeaceae), which gave an ED50 of 0.14 pg/mL, and from it genkwanin, (+)-lariciresinol and sitoindoside II were isolated as active principles by a bioassay-guided fractionation. The ED50 values of the compounds were 18.3, 1.1 and 0.069 pM, respectively. 

In 1991, the effects of triptolide (79) on the colony formation of breast cancer (MCFG-7 and BT-20), stomach cancer (Ml -45, MKN-7, and KATO-III), and promyelocytic leukemia (HL-60) cell lines, were reported [172]. The magnitudes of the inhibitory effect of triptolide on both breast and stomach cancer cell lines were similar to that of the leukemia cell line HL-60 (IC50 0.504-1.22 ig/1). The results obtained suggested that triptolide might have a potential therapeutic effect on some types of solid tumors, e.g., breast and stomach cancers. 

Surh YJ, Hurh YJ, Kang JY, Lee E, Kong G, Lee SJ. Resveratrol, an antioxidant present in red wine, induces apoptosis in human promyelocytic leukemia (HL-60) cells. Cancer Lett 1999 140 1-10. 

Promotion of cellular differentiation is a promising postulated mechanism for chemoprevention. Several compounds have been reported to induce cell differentiation, including retinoids, vitamin D3, and genistein. " Resveratrol is capable of inducting differentiation in some hematological malignant cell lines, colon cancers, and neuroblastoma. Jang et al. reported that incubation of the promyelocytic leukemia HL-60 cells with resveratrol induces cell differentiation and reduction of DNA synthesis. 

Peiez-Sacau, E., R.G. Diaz-Penate, A. Estevez-Braim, et al. 2007. S)mthesis and pharmacophore modeling of naphthoquinone derivatives with cytotoxic activity in human promyelocytic leukemia HL-60 cell Une. /. Med. Chem. 50(4) 696-706. 

Kim SY, Gao JJ, Kang HK (2000) Two flavonoids from the leaves of Moms alba induce differentiation of the human promyelocytic leukemia (HL-60) cell line. Biol Pharm BtrU 23(4) 451 55 Kim SR, Park MJ, Lee MK, Stmg SH, Park EJ, Kim J (2(X)2) Havonoids of Imda britannica protect cultured cortical cells from necrotic cell death induced by glutamate. Free Radic Biol Med 32(7) 596-604 Kim HK, Choi YH, Erkelens C, Lefeber AW, Verpoorte R (2005) Metabolic fingerprinting of Ephedra species using H-NMR spectroscopy and principal component analysis. Chem Pharm BtrU 53(1) 105-109... 

NAKAI, induces the differentiation of human acute promyelocytic leukemia HL-60 cells. Biol Pharm Bull 25 1446... 

Lu HF, Hsueh SC, Yu FS, Yang JS, Tang NY, Chen SC, Chung JG (2006) The role of Ca2-l- in (-)-menthol-induced human promyelocytic leukemia HL-60 cell death. In Vivo 20 69... 

Won K-J, Chung K-S, Lee YS, Alia MS, Pervez MK, Fatima S, Choi J-H, Lee K-T (2010) Haplophytin-A induces caspase-8-mediated apoptosis via the formation of death-inducing signaling complex in human promyelocytic leukemia HL-60 cells. Chem Biol Interact 188 505-511... 

Kim SY, Gao JJ, Kang HK. Two flavonoids from the leaves of Moms alba induce differentiation of the human promyelocytic leukemia (HL-60) cell line. Biol Pharm Bull 2000 23 451 55. 

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