Dermal microvascular endothelial cells

HDMEC human dermal microvascular endothelial cell... 

Li J, Zhou L, Tran HT, Chen Y, Nguyen NE, Karasek MA, et al. Overexpression of laminin-8 in human dermal microvascular endothelial cells promotes angiogenesis-related functions. J Invest Dermatol 2006 126 432-440. 

ALP alkaline phosphatase, Cht chitosan, DPM direct polymer melt, HDMECs human dermal microvascular endothelial cells, HUVECs human umbilical vein endothelial cells, MSCs mesenchymal stem cells, nHA nanocrystalline hydroxyapatite, PBS poly(butylene succinate), PCL poly(e-caprolactone), PGA poly (glycolic acid), PLA Poly(lactic acid), PLGA poIy(D,L-lactic-co-glycolic acid), PLCL poly(L-lactide-co-e-caprolactone)... 

As another alternative source of readily available endothelial cells of relative constant composition to create a human BBB model, we also used pooled neonatal human dermal microvascular endothelial cells (Cambrex, HMVEC-d, Cat. CC-2516). Briefly, these cells should be cultured in uncoated flasks in the recommended media by the supplier (EGM-2MV, Cat. CC-2516), for not more than six passages. Then, the cells should be passaged to astrocyte-containing filters just as described for the current model in EGM-2 MV instead of DMEM -l- S. After 5 days of co-culture with rat astrocytes and addition of... 

Human dermal microvascular endothelial cells produce matrix metalloproteinases in response to angiogenic factors and migration. J Invest Dermatol 105 170-176. 

Fig. 31 Rate of surface-mediated protein C activation for TM reconstituted into a poly (acrylatePC) HBM at a protein surface coverage of 170fmolcm 2 (sample) compared to confluent endothelial cell monolayers (HDMEC, human dermal microvascular endothelial cells HUVEC, human umbilical vein endothelial cells BAEC, bovine aortic endothelial cells). Reprinted with permission from [139], Copyright 2002, American Chemical Society...

Recent data from our group show that dermal microvascular endothelial cells isolated from SPHKl-deficient mice are defective in angiogenesis induced by VEGF and SIP (but not by FGF and TNF-a) in vitro, and that SPHKl knock-out mice show defective neoangiogenesis in vivo (S.Niwa, manuscript in preparation). 

To characterize the antitumor and/or antimetastatic activities, the effects of carp oil and oleic acid on DNA synthesis in LLC cells, adherence of LLC cells to human adult dermal microvascular endothelial cells (HMVEC), and Matrigel-induced angiogenesis (in vitro and in vivo) were tested. Carp oil inhibited DNA synthesis in LLC cells at 100 and 1000 pg/mL, but not at 0.1 to 10 pg/mL Table (10) . Oleic acid inhibited DNA synthesis in LLC cells at 1000 pM Table (11) . Carp oil inhibited the Matrigel-induced tube-like network formation of HMVEC at 1000 pg/mL, but not at 0.1 to 100 pg/mL Table (10) . 

Retroviral-mediated transduction of liTERT (human telomerase reverse transcriptase) in human dermal microvascular endothelial cells (EC) (HDMEC) results in cell hues that form microvasculai stractures upon subcutaneous implantation in severe combined immunodeficiency mice (SCID) mice. Anti-human type IV collagen basement membrane immu-noreactivity and visualization of enhanced green fluorescent protein (eGEP)-labeled microvessels confiimed the human origin of these vessels. Piimaiy HDMEC-derived vessel density decreased with time, while telomerized HDMEC maintained durable vessels six... 

Cheng SS, Lukacs NW, Kunkel SL Eotaxin/CCLll suppresses IL-8/CXCL8 secretion from human dermal microvascular endothelial cells. J Immunol 2002 168 2887. 

Silica seems to be a potent activator of endothelial cells in vitro, too. Incubation of human dermal microvascular endothelial cells (HDMEC) with silica at non-toxic concentrations increased the steady-state levels of the messenger RNA (mRNA) for intercellular adhesion molecule 1 (ICAM-i), and also increased the corresponding levels of this cell-surface protein as shown by fluorescence-activated cell-sorter (FACS) analysis the incubation also increased the level of soluble protein in the culture fluid, as shown by means of ELISA, in a dose- and time-dependent manner. Additionally, increased levels of IL-6 in the culture supernatants have been found. In addition, a significant increase in coUagenase I mRNA in HDMEC has been demonstrated (Anderegg et al. 1997). 

Cornelius LA, Sepp N, Li LJ, et al. Selective upregulation of intercellular adhesion molecule (ICAM-1) by ultraviolet B in human dermal microvascular endothelial cells. / Invest Dermatol. 1994 103(1) 23—28. 

Supp, D.M., Wilson-Landy, K., and Boyce, S.T., Human dermal microvascular endothelial cells form vascular analogs in cultured skin substitutes after grafting to athymic mice, FASEB J., 16,797,... 

Richard L, Velasco P, Detmar M. A simple immunomagnetic protocol for the selective isolation and long-term culture of human dermal microvascular endothelial cells. Exp Cell Res 1998 240 1-6. 

Hoting E, Paul E, Plewig G (1986) Treatment of rosacea with isotretinoin. Int J Dermatol 25 660-663 Imcke E, Ruszczak Zb, Mayer-da-Silva A, Detmar M, Orfanos CE (1991) Cultivation of human dermal microvascular endothelial cells in vitro Inununocytochemical and ultrastructural characterization and effect of treatment with three synthetic retinoids. Arch Dermatol Res 283 149-157 Jansen T, Plewig G (1997) Advances and perspectives in acne therapy. EurJ Med Res 28 321-334 Jones G, McLean J, Rosenthal D, Roberts J, Sander DN (1992) Combined treatment with oral etretinate and electron beam therapy in patients with cutaneous T-cell lymphoma (mycosis fungoides and Sezary syndrome). J Am Acad Dermatol 26 960-967... 

From the outset it was necessary that any biodegradable polyurethane intended for use as a dermal substitute would need to be nontoxic to cells (noncytotoxic) and support the growth of fibroblasts (and their production of collagen) and keratinocytes. Ultimately, in the wound, the matrix would need to sustain blood vessel in-growth to supply the overlying skin-grafted epidermis with nutrients, oxygen, immune cells, and factors, and transport out the toxic by-products of cellular respiration. Therefore, microvascular endothelial cells (MVECs) would also need to thrive in the presence of BTM. 

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