Matrigel-induced angiogenesis

To characterize the antitumor and/or antimetastatic activities, the effects of carp oil and oleic acid on DNA synthesis in LLC cells, adherence of LLC cells to human adult dermal microvascular endothelial cells (HMVEC), and Matrigel-induced angiogenesis (in vitro and in vivo) were tested. Carp oil inhibited DNA synthesis in LLC cells at 100 and 1000 pg/mL, but not at 0.1 to 10 pg/mL Table (10) . Oleic acid inhibited DNA synthesis in LLC cells at 1000 pM Table (11) . Carp oil inhibited the Matrigel-induced tube-like network formation of HMVEC at 1000 pg/mL, but not at 0.1 to 100 pg/mL Table (10) . 

Values are expressed as means S.E. of 4 experiments. P<0.05, Significantly different from medium alone. Table 12. Effects of carp oil on Matrigel-induced angiogenesis (in vivo)1 ... 

Table 13. Effects of oleic acid on Matrigel-induced angiogenesis (in vivo)1...

Table 10. Effects of active substances from A hlazei on Matrigel-induced angiogenesis...

Table 2. Effects of fucoidan on angiogenesis induced by Matrigel supplemented with aFGF and heparin1...

Apart from fibrin, collagen, and alginate, other natural polymers that have shown promising results in vivo are chitosan, Matrigel, hyaluronic acid, and decellularized ECM [14]. For example, Fujita et al. showed efficient angiogenesis and collateral flow induced by FGF-2 loaded in chitosan HGs. Notably, the chitosan HG allowed an extended FGF-2 delivery for more than 1 month [76]. Moreover, chitosan HGs combined with stem cells also resulted in increased angiogenesis, reduced adverse remodeling and preserved heart function compared to free cells or nonloaded chitosan HG administration [77]. 

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