Colon adenocarcinoma

A 2-h incubation with another PGE2 analogue, nocloprost (9P-chloro-DMPG) protects normal human fibroblasts but has no effect on the survival of colon adenocarcinoma cells exposed to 10 Gy (1000 rad) (218). Nocloprost protects against radiation-induced DSBs in normal cells but not in tumor cells. Moreover, incubation using nocloprost for 2 h after irradiation enhances the rate of DSB rejoining in fibroblasts but not in adenocarcinoma cells. These data possibly reflect a different distribution of PG receptors on the plasma membrane of the two cell types. 

CLARKE R G, LUND E K, JOHNSON I T aud PINDER A c (2000) Apoptosis cau he detected in attached colonic adenocarcinoma HT29 cells using annexin V binding, hut uot hy TUNEL assay or suh-GO DNA content . Cytometry, 39 141-50. 

PALOZZA P, SERINI S, MAGGIANO N, ANGELINI M, BONINSEGNA A, DI NICUOLO F, RANELLETTI F O and CALVIELLO G (2002) Induction of cell cycle arrest and apoptosis in human colon adenocarcinoma cell lines by P-carotene through down-regulation of cyclin A and Bcl-2 family proteins , Carcinogenesis, 23, 11-18. 

CCR2 expression was also examined in liver tumor formation induced by intraportal injection of a murine colon adenocarcinoma cell line (58). Tumor growth occurred at similar rates in wild-type and CCR2 /" mice, but the number... 

O Sullivan SM, Woods JA, and O Brien NM. 2004. Use of Tween 40 and Tween 80 to deliver a mixture of phytochemicals to human colonic adenocarcinoma cell (CaCo-2) monolayers. British Journal of Nutrition 91(5) 757-764. 

Palozza, P, S Serini, A Torsello et al. 2003. Beta-carotene regulates NF-kappa B DNA-binding activity by a redox mechanism in human leukemia and colon adenocarcinoma cells. J Nutr 133 381-388. 

In a recent study, the antiproliferative effect of different carotenoids, including (3-carotene, lycopene and lutein, on PCNA and cyclin Dl expression in human KB cells have been studied. The results indicate that carotenoids suppressed cell growth by acting as inhibitors of the expressions of PCNA and cyclin Dl, although in a different extent (Cheng et al., 2007). On the other hand, (3-carotene was able to induce a cell cycle delay in G2/M phase by decreasing the expression of cyclin A in human colon adenocarcinoma cells (Palozza et al., 2002a). 

Palozza, P., Serini, S., Boninsegna, A. et al. 2007a. The growth-inhibitory effects of tomatoes digested in vitro in colon adenocarcinoma cells occur through down regulation of cyclin DI, Bcl-2 and Bcl-xl. Br J Nutr 98 789-795. 

Yan, F., Subramanian, B., Nakeff, A., Barder, T.J., Parus, S.J., Lubman, D.M. (2003). A comparison of drug-treated and untreated HCT-116 human colon adenocarcinoma cells using a 2-D liquid separation mapping method based on chromatofocusing pi fractionation. Anal. Chem. 75, 2299-2308. 

Zhang, Z., Krylov, S., Arriaga, E.A., Polakowski, R., Dovichi, N.J. (2000). One-dimensional protein analysis of an HT29 human colon adenocarcinoma cell. Anal. Chem. 72, 318-322. 

FIGURE 1.2 2D liquid protein expression map of the HCT-116 human colon adenocarcinoma cell line. (See text for full caption.)... 

Human colon adenocarcinoma cell line used as an absorption model... 

Y., Function and expression of multidrug resistance-associated protein family in human colon adenocarcinoma cells (Caco-2),... 

Peters, W. H. N., Roelofs, H. M. J., Time-dependent activity and expression of glutathione S-transferases in the human colon adenocarcinoma cell line Caco-1, Biochem. J. 1989, 264, 613-616. 

Colon adenocarcinoma epithelial cell line Rat renal proximal tubular epithelial cell line Tetrahydrofuranyl Valacyclovir hydrolase... 

The human colon adenocarcinoma cell line (Caco-2) assay is still commonly used to measure the potential for a compound to be absorbed. It measures the permeability potential because permeability is a component of the absorption process,3 27 31 Multiple reports discuss the use of HPLC/MS/MS to support the Caco-2 assay.32 38... 

Samuelsson78 (Sweden) used ECT to destroy colon adenocarcinoma tumors in rats by applying 10 40 mA currents for 1 h and using one to three treatments. Torpedo shaped platinum electrodes were used, with an anode inserted in the centre of the tumor and a cathode placed at the tumor surface. The results obtained by ECT were comparable to those obtained by surgery 50% of the rats treated with either ECT or surgery were devoid of tumors when killed 7 months after inoculations that induced the tumors. 

Caco-2 human Caucasian colon adenocarcinoma cells... 

P5. Pyke, C., Kristensen, P., Ralfkiaer, E., Grondahl-Hansen, J., Eriksen, K., Blasi, F., and Dano, K., Urokinase-type plasminogen activator is expressed in stromal cells and its receptor in cancer cells at invasive foci in human colon adenocarcinomas. Am. J. Pathol. 138, 1059— 1067 (1991). 

Caco-2 Human colon adenocarcinoma Well-established cell model Differentiates spontaneously and expresses some relevant efflux transporters (e.g., P-gp, MRP1-2, BCRP) Interlaboratory differences... 

Polarising Caco-2 LLC-PK1 MDCK Human colon adenocarcinoma, epithelial, enterocyte-like Porcine kidney, epithelial, proximal tubule cell-like Canine kidney, epithelial, distal tubule cell-like... 

In MDR cells, a significant fraction of P-gp is found associated with caveolin-rich membranes, and there is a substantial increase in the number of caveolae and caveolin-1 protein level. For example, both multidrug resistant human colon adenocarcinoma HT-29 cells and adriamycin-resistant breast adenocarcinoma MCF-7 cells display about a 12-fold increase in caveolin expression, which correlates with an approximate fivefold increase in morphologically identifiable caveolae [55], In addition, these cells exhibit increased amounts of phospholipase D and lipids such as cholesterol, glucosylceramide, and sphingomyelin [56, 57], Similarly, taxol-resistant A549 cells display both increased caveolin expression and caveolae numbers [58], While these correlations track with MDR, they do not suggest a simple mechanism for the role of... 

Cell monolayers grown on permeable culture inserts form confluent mono-layers with barrier properties and can be used for drug absorption experiments. The most well-known cell line for the in vitro determination of intestinal drug permeability is the human colon adenocarcinoma Caco-2 [20, 21], The utility of the Caco-2 cell line is due to its spontaneous differentiation to enterocytes under conventional cell culture conditions upon reaching confluency on a porous membrane to resemble the intestinal epithelium. This cell model displays small intestinal carriers, brush borders, villous cell model, tight junctions, and high resistance [22], Caco-2 cells express active transport systems, brush border enzymes, and phase I and II enzymes [22-24], Permeability models... 

Other cell lines used in permeability studies include the T84 human colonic adenocarcinoma colonic crypt cell model. This line has a reduced carrier expression, secrets mucus, and has very high resistance [31, 32], The IEC cell line is a rat fetal intestinal epithelium cell with higher permeabilities than Caco-2 cells [33], LLC PKi is a pig kidney epithelial cell line with low expression of efflux systems, but expression systems for transport proteins [32], 2/4/A1 cells are a conditionally immortalized rat fetal intestinal epithelium line with crypt cell-like morphology and temperature-sensitive differentiation [34], They form differentiated monolayers with tight junctions, increased brush border enzymes when grown on extracellular matrices with laminin. Transport of drugs with LP in 2/4/A1 monolayers was comparable to that in the human jejunum and up to 300 times faster than that in Caco-2 monolayers. In contrast, the permeability of HP drugs was comparable in both cell lines [34],... 

Pinto, M., Robine-Leon, S., Appay, M.D., Kedinger, M., Triadou, N., Dussaulx, E., Lacroix, B., Simon-Assmann, P., Haffen, K., Fogh, and Zweibaum, A., Enterocytic-like differentiation and polarization of the human colon adenocarcinoma cell line Caco-2 in culture, Biol. Cell, 47,... 

Hirohashi, T., Suzuki, H., Chu, X.-Y., Tamai, I., Tsuji, A., and Sugiyama, Y., Function and expression of multidrug resistance-associated protein family in human colon adenocarcinoma cells (Caco-2), /. Pharmacol. Exp. Ther., 292, 265, 2000. 

Stoka, V., Turk, B., Schendd, S.L., Kim, T.-H., Cirman, T, Srripas, SJ., EUerby, L.M., Bredesen, L., Freeze, H, Abrahamson, M., Bromme, D., Krajewski, S., Reed, J.C., Yin, X.-M., Turk, V., and Salvesen G.S., 2001, Lysosomal Protease Pathways to Apoptosis. Cleavage of Bid, not pro-caspases, is the most likely route. J. Biol. Chem. 276 3149-3157 Srm, X. and Ross, D., 1996, Quinone-induced apoptosis in human colon adenocarcinoma ceUs via DT-diaphorase mediated bioactivation. Chem. Biol. Interact. 100 267-76 Taatjes, D.J. and Koch, T.H., 2001, Nuclear targeting and retention of anthracycUne antitumor dmgs in sensitive and resistant tumor ceUs. Curr. Med Chem. 8 15-29 Tarr, M. and van Helden, PT)., 1990, Inhibition of transcription by adriamycin is a... 

B. S. Reddy, T. Narasawa, J. H. Weisburger and E. L. Wynder, Promoting effect of sodium deoxycholate on colon adenocarcinomas in germfree rats, J. Natl. Cancer Inst., 1976, 56(2), 441. 

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