Colon adenocarcinoma cell line assay

The human colon adenocarcinoma cell line (Caco-2) assay is still commonly used to measure the potential for a compound to be absorbed. It measures the permeability potential because permeability is a component of the absorption process,3 27 31 Multiple reports discuss the use of HPLC/MS/MS to support the Caco-2 assay.32 38... 

Raubenheimer reported the anti-cancer activity of compound 34 (Figure 4.9).This complex was tested on human cervical epithelioid carcinoma cell line HeLa, human colon adenocarcinoma cell line CoLo 320 DM, leukaemia cell line Jurkat, and breast cancer cell line MCF-7. IC50 values determined by the MTT assay showed human lymphocytes were less sensitive than the cancer cells tested, indicating some selectivity for the cancer cells. 

The prediction of the important structural features that affect intestinal permeability is useful information to obtain early in the drug discovery process. The two most common models used to obtain fast, high-throughput measurements are the parallel artificial membrane permeation assay (PAMPA) and the cell line assays that feature cultured human colon adenocarcinoma cells (Caco-2). Each method uses a surrogate model to mimic intestinal absorption followed by LC-MS analysis. 

Brisdelli et al. (2013) investigated the effects of six lichen metabolites (diffractaic acid, lobaric acid, usnic acid, vicanicin, variolaric acid, protolichesterinic acid) on reactive oxygen species (ROS) level towards three human cancer cell lines, MCF-7 (breast adenocarcinoma), HeLa (cervix adenocarcinoma) and HCT-116 (colon carcinoma). AU tested lichen compounds did not exhibit free radical scavenging activity using the l,l-diphenyl-2-picrylhydrazyl (DPPH) assay. The lichen metabolites did not significantly increase the intracellular ROS level and did not prevent oxidative injury induced by t-butyl hydroperoxide in tested cells. 

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