Adenocarcinoma cells

A 2-h incubation with another PGE2 analogue, nocloprost (9P-chloro-DMPG) protects normal human fibroblasts but has no effect on the survival of colon adenocarcinoma cells exposed to 10 Gy (1000 rad) (218). Nocloprost protects against radiation-induced DSBs in normal cells but not in tumor cells. Moreover, incubation using nocloprost for 2 h after irradiation enhances the rate of DSB rejoining in fibroblasts but not in adenocarcinoma cells. These data possibly reflect a different distribution of PG receptors on the plasma membrane of the two cell types. 

In order to assess the effect of the corn cob xylan on the cell viability and proliferation rate, xylan solutions at concentrations of 0.1, 0.25, 0.50, 0.75, and 1 mg/ml were placed in contact with human cervical adenocarcinoma cells (HeLa cells) for 24 and 72 h. Finally, the cell viability was determined by the MTT assay. It was observed that regardless of the xylan concentration, the samples tested did not affect the viability of HeLa cells after incubation for 24 h (Figure 13) (Unpublished data). 

The test system was considerably less sensitive to endosulfan when mouse ER, rather than human ER, was used to mediate (3-gal activity (Ramamoorthy et al. 1997). In similar assays, endosulfan at 10 jM had no effect on (3-gal activity in yeast Saccharomyces) transfected with either the human or rainbow trout ER (Andersen et al. 1999). In addition, no effect was observed on transcriptional activation of HeLa cells transfected with plasmids containing an estrogen receptor as a responsive element (Shelby et al. 1996). Endosulfan also did not induce transient reporter gene expression in MCF-7 human breast cancer cells at an incubation concentration of 2.5 pM (Andersen et al. 1999). Maximum endosulfan-induced ER-mediated luciferase reporter gene expression occurred in vitro in a T47D human breast adenocarcinoma cell line at approximately 10 pM, while 50% expression of luciferase occurred at about 5.9 pM the maximum expression was approximately 59% of the effect from exposure to 0.03 nM estradiol (0.00003 pM) (Legler et al. 1999). Luciferase expression from combined treatment with endosulfan and dieldrin was additive over concentrations ranging from 3 to 8 pM. 

LUND E K, SMITH T K and JOHNSON I T (2000) The mechanism of isothiocyanate induced death of HT29 adenocarcinoma cells is dependent on the nature of the side chain , Gastroenterology, 118 2895. 

PALOZZA P, SERINI S, MAGGIANO N, ANGELINI M, BONINSEGNA A, DI NICUOLO F, RANELLETTI F O and CALVIELLO G (2002) Induction of cell cycle arrest and apoptosis in human colon adenocarcinoma cell lines by P-carotene through down-regulation of cyclin A and Bcl-2 family proteins , Carcinogenesis, 23, 11-18. 

Apoptosis of cultured hmnan endometrial adenocarcinoma cells and inhibition of cell proliferation (Miyoshi et al.,... 

FAN H, MORIOKA T, iTO E (2000) Induction of apoptosis and growth inhibition of cultured human endometrial adenocarcinoma cells (Sawano) by an anti-mmor lipoproteinfraction of rice bran. Gynacol, Oncol, 76(2) 170-75. 

Yeh, S.L. and Hu, M.L., Oxidized [beta]-carotene inhibits gap junction intercellular communication in the human lung adenocarcinoma cell line A549, Food Chem. Toxicol., 41, 1677, 2003. 

Caco-2 adenocarcinoma cell line derived from human colon... 

CCR2 expression was also examined in liver tumor formation induced by intraportal injection of a murine colon adenocarcinoma cell line (58). Tumor growth occurred at similar rates in wild-type and CCR2 /" mice, but the number... 

Goldberg-Bittman L, Neumark E, Sagi-Assif O, et al. The expression of the chemokine receptor CXCR3 and its ligand, CXCL10, in human breast adenocarcinoma cell lines. Immunol Lett 2004 92 171-178. 

O Sullivan SM, Woods JA, and O Brien NM. 2004. Use of Tween 40 and Tween 80 to deliver a mixture of phytochemicals to human colonic adenocarcinoma cell (CaCo-2) monolayers. British Journal of Nutrition 91(5) 757-764. 

Palozza, P, Calviello, G, Serini, S, Maggiano, N, Lanza, P, Ranelletti, FO, and Bartoli, GM, 2001. Beta-carotene at high concentrations induces apoptosis by enhancing oxy-radical production in human adenocarcinoma cells. Free Radic Biol Med 30, 1000-1007. 

Palozza, P, S Serini, A Torsello et al. 2003. Beta-carotene regulates NF-kappa B DNA-binding activity by a redox mechanism in human leukemia and colon adenocarcinoma cells. J Nutr 133 381-388. 

In a recent study, the antiproliferative effect of different carotenoids, including (3-carotene, lycopene and lutein, on PCNA and cyclin Dl expression in human KB cells have been studied. The results indicate that carotenoids suppressed cell growth by acting as inhibitors of the expressions of PCNA and cyclin Dl, although in a different extent (Cheng et al., 2007). On the other hand, (3-carotene was able to induce a cell cycle delay in G2/M phase by decreasing the expression of cyclin A in human colon adenocarcinoma cells (Palozza et al., 2002a). 

Palozza, P., Serini, S., Boninsegna, A. et al. 2007a. The growth-inhibitory effects of tomatoes digested in vitro in colon adenocarcinoma cells occur through down regulation of cyclin DI, Bcl-2 and Bcl-xl. Br J Nutr 98 789-795. 

Yan, F., Subramanian, B., Nakeff, A., Barder, T.J., Parus, S.J., Lubman, D.M. (2003). A comparison of drug-treated and untreated HCT-116 human colon adenocarcinoma cells using a 2-D liquid separation mapping method based on chromatofocusing pi fractionation. Anal. Chem. 75, 2299-2308. 

As an example, the data computed on colorectal adenocarcinoma cell line map (DLD1 HUMAN) (Demalte-Annessi et al., 1999 Pietrogrande et al., 2002). (Fig. 4.1a) are reported as follows. 

It must be emphasized that the availability of the SMO and 2D autocovariance function methods as two independent statistical procedures to estimate the same parameter, in, the number of proteins, is a helpful tool to verify the reliability of the results obtained. In the case of the 2D PAGE map of colorectal adenocarcinoma cell line (DL-1) an excellent agreement was found between the values obtained from the SMO method—m = 101 10 and m = 105 10—and the 2D autocovariance function procedure—m = 104 10 (Pietrogrande et al., 2006a). 

Langdon, S. P, Hawkes,M. M., Hay,F. G.,Lawrie, S. S., Schol, D. J., Hilgers, J.,Leonard, R. C. F., Smyth, J. F. (1988). Effect of sodium-hutyrate and other differentiation inducers on poorly differentiated human ovarian adenocarcinoma cell-lines. Cancer Res. 48(21), 6161— 6165. 

Zhang, Z., Krylov, S., Arriaga, E.A., Polakowski, R., Dovichi, N.J. (2000). One-dimensional protein analysis of an HT29 human colon adenocarcinoma cell. Anal. Chem. 72, 318-322. 

FIGURE 1.2 2D liquid protein expression map of the HCT-116 human colon adenocarcinoma cell line. (See text for full caption.)... 

Human colon adenocarcinoma cell line used as an absorption model... 

Y., Function and expression of multidrug resistance-associated protein family in human colon adenocarcinoma cells (Caco-2),... 

Peters, W. H. N., Roelofs, H. M. J., Time-dependent activity and expression of glutathione S-transferases in the human colon adenocarcinoma cell line Caco-1, Biochem. J. 1989, 264, 613-616. 

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