Cloning and characterization

Morishita, H., et al. (2002). Cloning and characterization of an active fragment of luciferase from a luminescent marine alga, Pyrocystis lunula. Photochem. Photobiol. 75 311-315. 

Nakajima, Y., etal. (2004). cDNA cloning and characterization of a secreted luciferase from the luminous Japanese ostracod, Cypridina noctiluca. Biosci. Biotechnol. Biochem. 68 565-570. 

Sarge, K.D., Zimarino, V., Holm, K., Wu, C Morimoto, R.l. (1991). Cloning and characterization of two mouse heat shock factors with distinct inducible and constitutive DNA binding ability. Genes Dev. 5, 1902-1911. 

Harpster, M.H. Taylor, W.C. (1986). Maize phosphoenolpyruvate carboxylases. Cloning and characterization of mRNAs encoding isozymic forms. Journal of Biological Chemistry, 261, 6132-6. 

S Molecular cloning and characterization of a novel 62 type of histamine receptor preferentially expressed in leukocytes. J Biol Chem 2000 275 36781. 

Dal G, Carrasco L, Carrasco N Cloning and characterization of the thyroid iodide transporter. Nature 1996 379 458. 

Sprenger, G.A. et al.. Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol, Proc. Natl. Acad Sci. USA 94, 12857, 1997. Lange, B.M. et al., A family of transketolases that directs isoprenoid biosynthesis via a mevalonate-independent pathway, Proc. Natl. Acad Sci. USA 95, 2100, 1998. Lois, L.M. et al., Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1- deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis, Proc. Natl. Acad. Sci. USA 95, 2105, 1998. 

Pecker, I. et al.. Cloning and characterization of the cDNA for lycopene b-cyclase from tomato reveals decrease in its expression during fruit ripening. Plant Mol. Biol. 30, 807, 1996. 

Li, Z.H. et ah. Cloning and characterization of a maize cDNA encoding phytoene desaturase, an enzyme of the carotenoid biosynthetic pathway. Plant Mol. Biol. 30, 269, 1996. 

Li, Z.H., Molecular cloning and characterization of phytoene desaturase cDNA and leucine-rich repeat protein kinase cDNA from maize, PhD dissertation. Graduate School and University Center, City University of New York, 1998. 

Verdoes, J.C., Misawa, N., and van Ooyen, A.J., Cloning and characterization of the astaxanthin biosynthetic gene encoding phytoene desaturase of XanthophyUomyces dendrorhous [letter], Biotechnol. Bioeng. 63, 750, 1999. 

Kofod LV, Kauppinen S, Christgau S, Andersen LN, Heldt-Hansen HP, Dorreich K, Dalboge (1994) Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus. J Biol Chem 269 29182-29189... 

Here we describe the purification of RHG, the cloning and characterization of the corresponding gene and (over)production of the enzyme in A. aculeatus and Aspergillus awamori. 

At this time, more then thirty channel DNAs have been cloned and characterized from various sources, predominantly from Drosophila melanogaster, mouse, rat and human cDNA/genomic libraries [6-31]. Inspection of the derived primary K channel protein sequences indicates that voltage-gated channels belong to a... 

Historically the Shaker (Sh) K channel was the first K channel which was cloned and characterized [6-10]. Subsequently many more channel cDNAs and genes have been isolated and studied. Yet Sh channels remained in the forefront of channel research. The study of Sh channel mutants has provided the most thorough insight into structure-function relationships of K channels to date. I will first discuss in this chapter the primary sequences of voltage-gated channels. I will only use a few selected examples for discussion. As of this time, so many related K channel protein sequences have been published that it is not feasible to discuss all of them. Subsequently, I will describe in detail the present knowledge about functional K" " channel domains which are implicated in activation, inactivation and selectivity of the channel. 

Iwaki H, Y Hosegawa, S Wang, MM Kayser, PCK Lau (2002) Cloning and characterization of a gene cluster involved in cyclopentanol metabolism in Comamonas sp. strain NCIMB 9872 and biotransformations effected by Escherichia co/f-expressed cyclopentanone 1,2-monooxygenase. Appl Environ Microbiol 68 5671-5684. 

Kostichka K, SM Thomas, KJ Gibson, V Nagarajan, Q Cheng (2001) Cloning and characterization of a gene cluster for cyclododecanone oxidation in Rhodococcus ruber SCI. J Bacterial 183 6478-6486. 

Miyauchi K, H-S Lee, M Fukuda, M Takagi, Y Nagata (2002) Cloning and characterization of linR, involved in regulation of the downstream pathway for y-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26. Appl Environ Microbiol 68 1803-1807. 

Blumel S, H-J Knackmuss, A Stolz (2002) Molecular cloning and characterization of the gene coding for the aerobic azoreductase from Xenophilus azovorans KF46F. Appl Environ Microbiol 68 3948-3955. 

Van der Meer JR, ARW van Neerven, EJ de Vries, WM de Vos, AJB Zehnder (1991) Cloning and characterization of plasmid-encoding genes for the degradation of 1,2-dichloro-, 1,4-dichloro-, and 1,2,4-trichlo-robenzene of Pseudomonas sp. strain P51. J Bacterial 173 6-15. 

Hughes MA, PA Williams (2001) Cloning and characterization of the pnb genes, encoding enzymes for 4-nitrobenzoate catabolism in Pseudomonas putida TW3. J Bacteriol 183 1225-1232. 

Kawasaki K, A Yokota, S Oita, C Kobayashi, S Yoshikawa, S Kawamoto, S Takao, F Tomita (1993) Cloning and characterization of a tryptophanse gene from Enterobacter aerogenes SM-18. J Gen Microbiol 139 3275-3281. 

Utsumi R et al. (1991) Molecular cloning and characterization of the fusaric acid-resistance gene from Pseudomonas cepacia. Agric Biol Chem 55 1913-1918. 

D. Tagu. B. Nasse, and F. Martin, Cloning and characterization of hydrophobins-encoding cDNAs from the ectomycorrhizal basidiomycete Pi.solithiis tinctorius. Gene 168 93 (1996). 

H. Liu, A. T. Trieu, L. A. Blaylock, and M. J. Harrison, Cloning and characterization of two phosphate transporters from Medicago truncatula roots Regulation in response to phosphate and to colonization by arbuscular mycorrhizal fungi. Mol. Plant-Microbe Interact. 11 A (1998). 

Fu W, Zhang Y, Zhang J, Chen WF. Cloning and characterization of mouse homolog of the CXC chemokine receptor CXCR1. Cytokine 2005 31 9-17. 

Takahashi M, Jeevan A, Sawant K, McMurray DN, Yoshimura T. Cloning and characterization of guinea pig CXCR1. Mol Immunol 2007 44 878-888. 

Fan P, Kyaw H, Su K, et al. Cloning and characterization of a novel human chemokine receptor. Biochem Biophys Res Commun 1998 243 264-268. 

Nagasawa T, Nakajima T, Tachibana K, et al. Molecular cloning and characterization of a murine pre-B-cell growth-stimulating factor/stromal cell-derived factor 1 receptor, a murine homolog of the human immunodeficiency virus 1 entry coreceptor fusin. Proc Natl Acad Sci U S A 1996 93 14726-14729. 

Pines, J., and Hunt, T. (1987). Molecular cloning and characterization of the mRNA for cyclin from sea urchin eggs. EMBO J. 6 2987-2995. 

Witz, P., Amlaiky, N., Plassat, J. L., Maroteaux, L., Borrelli, E., and Hen, R. (1990). Cloning and characterization of a Drosophila serotonin receptor that activates adenylate cyclase. Proc. Natl. Acad. Sci. USA 87 8940-8944. 

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