Rhodococcus ruber DSM

The reduction of several ketones, which were transformed by the wild-type lyophilized cells of Rhodococcus ruber DSM 44541 with moderate stereoselectivity, was reinvestigated employing lyophilized cells of Escherichia coli containing the overexpressed alcohol dehydrogenase (ADH- A ) from Rhodococcus ruber DSM 44541. The recombinant whole-cell biocatalyst significantly increased the activity and enantioselectivity [41]. For example, the enantiomeric excess of (R)-2-chloro-l-phenylethanol increased from 43 to >99%. This study clearly demonstrated the advantages of the recombinant whole cell biocatalysts over the wild-type whole cells. 

Stampfer, W., Kosjek, B., Faber, K. and Kroutil, W. (2003) Biocatalytic asymmetric hydrogen transfer employing Rhodococcus ruber DSM 44541. The Journal of Organic Chemistry, 68 (2), 402-406. 

Van Deursen, R., Stampfer, W., Edegger, K. et al. (2004) Chemo- and stereo-selective biocatalytic reduction of a,/8-unsaturated ketones employing a chemo-tolerant ADH from Rhodococcus ruber DSM 44541. Journal of Molecular Catalysis B-Enzymatic, 31 (4-6), 159-163. 

Overall, retaining sec-alkylsulfatase activity has been detected in Planctomycetes spp. (such as Rhodopirellula baltica DSM 10527 complementary inverting sulfatase activity was found in Actinomycetes (e.g. Rhodococcus ruber DSM 44541 " ), Archaea (e.g. Sulfolobus spp. ) and pseudomonads. ... 

Pseudomonas spp. DSM 6611 and 6978 and Rhodococcus ruber DSM 44541 were obtained from DSMZ (Deutsche Stammsammlung fiir Mikroorganismen und Zellkulturen, Braunschweig, Germany, www.dsmz.de)... 

Pogorevc, M. and Faber, K., Enantioselective stereoinversion of rec-alkyl sulfates by an alkyl-sulfatase from Rhodococcus ruber DSM 44541. Tetrahedron Asymm., 2002,13, 1435. 

Organisms Lactobacillus kefir DSM 20587, Saccharomyces cerevisiae, Candida magnoliae, Bacillus megaterium, Thermoanaerobium brockii, Clostridium beijerinckii, Thermoanaerobacter ethanolicus, Rhodococcus ruber DSM 44541. Solvents ace = acetone iPr = i-PrOH. Substrates WM Wieland-Miescher ketone 4-Me-HP 4-methyl Hajos-Parrish ketone COBE ethyl 4-chloro-3-oxobutanoate. 

W. Stampfer, B. Kosjek, W. Kroutil, and K. Faber, On the organic solvent and thermostability of the biocatalytic redox system of Rhodococcus ruber DSM 44541, Biotechnol. Bioeng. 2003a, 81, 865-869. 

S.R., and Faber, K. (2002) Enantiose-lective stereoinversion in the kinetic resolution of rac-sec-alkyl sulfate esters by hydrolysis with an alkyl-sulfatase from rhodococcus ruber DSM 44541 furnishes homochiral products. Angew. Chem. Int. Ed., 41 (21), 4052-4054. 

IbADH Lactobacillus brevis ADH ADH-A ADH from Rhodococcus ruber DSM 44541 KRED CDX-026 commercial ADH, source not further specified, a) Reduced pressure to remove acetone. 

Karabec, M., Lyskowsld, A., Tauber, K.C., Steinkellner, G., Kroutil, W., Grogan, G., and Gruber, K. (2010) Structural insights into substrate spedfidly and solvent tolerance in alcohol dehydrogenase ADH- A from Rhodococcus ruber DSM 44541. Chem. Commun., 46, 6314—6316. 

Synthesis of (/ )-myrcenediol via an enantioconvergent biohydrolysis using Rhodococcus ruber DSM 44540 EH as a biocatalyst. 

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