Cancer tissue analysis

Keshavarzian, A., Zapeda, D., List, T. and Mobarhan, S. (1992d). High levels of reactive oxygen metabolites in colon cancer tissues analysis by chemiluminescence probe. Nutr. Cancer 17, 243-249. 

First, a limited study was performed using breast cancer tissue to establish an optimal protocol of DNA extraction for a-CGH analysis that would allow comparison of a-CGH results after boiling in different solutions three pH values of 7, 9, and 12 of Britton and Robinson buffer solution, and a 0.1 M sodium hydroxide solution. DNA samples extracted from frozen and from FFPE tissue sections by a nonheating protocol were employed, and the results were compared a protocol of boiling samples in 0.1 M sodium hydroxide gave optimal results. 

Daigo Y, Chin S-F, Gorringe KL, et al. Degenerate oligonucleotide primed-polymerase chain reaction-based array comparative genomic hybridization for extensive Amplicon profiling of breast cancers. A new approach for the molecular analysis of paraffin-embedded cancer tissue. Am. J. Pathol. 2001 158 1623-1631. 

Hood BL, Darfler MM, Guiel TG, et al. Proteomic analysis of formalin-fixed prostate cancer tissue. Mol. Cell. Proteomics 2005 4 1741-1753. 

The term proteome means the total protein complement of a genome, and pro-teomics means the analysis for proteome. The combination of two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) is a proteomic method of high-throughput analysis of protein expression. By using this 2-DE and MS, proteomic studies have identified many proteins that may be involved in the pathogenic mechanism of cancers. These studies analyzed cancer cell lines, as well as cancer tissues or serum from patients. 

In the present study, we analyzed proteome in hepatocellular carcinoma (HCC), esophageal cancer, and pancreatic cancer tissues. We identified many proteins whose expression in cancer tissues was different from corresponding non-cancerous tissues by using 2-DE and MS. Furthermore, we identified some auto-antibodies reacting to proteins in HCC cancer tissues. In this chapter, we will describe the method, our experimental result, and reports from other researchers about proteomic analysis in cancer patients. 

Overview of Experimental Procedure 3.1.1. Proteomic Analysis of Cancer Tissues... 

Kuramitsu Y, Nakamura K. Proteomic analysis of cancer tissues shedding light on carcinogenesis and possible biomarkers. Proteomics 2006 6 5650-5661. 

Hernandez-Blazquez, F. J., Habib, M., Dumollard, J.-M., Barthelemy, C., Benchaib, M., de Capoa, A., and Niveleau, A. 2000. Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis. Gwi47 689-693. 

Sabe, I., Andritsh, I., Mangoud, A., Awad, A. S., Khalifa, A., and Krishan, A. 1999. Flow cytometric analysis of estrogen receptor expression in isolated nuclei and cells from mammary cancer tissues. Cytometry 36 131-139. 

The IMS assay has been applied to the analysis of animal organ tissue, skin, whole body, human and animal cancer tissue, and drug formulation. It was also used for MS imaging study on mammalian cell [147], single neurons [148,149], bacteria [150], and MS imaging of features smaller than the size of laser beam [151],... 

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Dukor, R.K., liebman, M.N. and Johnson, B.L (1998) A new, non-destructive method for analysis of clinical samples with FT-IR microspectroscopy. Breast cancer tissue as an example. Cdl. Mol. Biol, 44, 211-17. 

Aslan G, Irer B, Tuna B, et al. Analysis of NKX3.1 expression in prostate cancer tissues and correlation with clinicopathologic features. Pathol Res Pract. 2006 202 93. 

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