Degenerate oligonucleotide-primed

Daigo Y, Chin S-F, Gorringe KL, et al. Degenerate oligonucleotide primed-polymerase chain reaction-based array comparative genomic hybridization for extensive Amplicon profiling of breast cancers. A new approach for the molecular analysis of paraffin-embedded cancer tissue. Am. J. Pathol. 2001 158 1623-1631. 

Stoecklein NH, Erbersdobler A, Schmidt-Kittler O, et al. SCOMP is superior to degenerated oligonucleotide primed-polymerase chain reaction for global amplification of minute amounts of DNA from microdissected archival tissue samples. Am. J. Pathol. 2002 161 43-51. 

Multiplex PCR of the muscular dystrophin gene was performed on-chip. The method of degenerate oligonucleotide-primed PCR (DOP-PCR) was first used to increase the number of DNA templates before multiplex PCR. Therefore, DOP-PCR can provide the template DNA from the whole human genome, but this procedure is feasible only when the amplicon size is less than 250 bp [931]. 

Cheng, J., Waters, L.C., Fortina, P., Hvichia, G., Jacobson, S.C., Ramsey, J.M., Kricka, L.J., Wilding, P., Degenerate oligonucleotide primed-polymerase chain reaction and capillary electrophoretic analysis of human DNA on microchip-based devices. Anal. Biochem. 1998, 257(2), 101-106. 

Yeast artificial chromosomes (YACs) can also be isolated and labeled in this manner, but it is difficult to prepare sufficient quantities of YAC DNA without employing an amplification scheme. One solution is to use a nonspecific, degenerate-oligonucleotide-primed PCR [DOP-PCR (Telenius et al. 1992)] method to amplify templates such as gel-purified YACs or even flow-sorted chromosomes. 

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