Baculovirus Expression Systems

Bryant Yes, although we didn t use purified IDGF, but recombinant IDGF produced from a baculovirus expression system. These are clone 8 cells from an imaginal disc we haven t tried growing the whole imaginal disc. 

Zhang, F., Saarinen, M.A., Itle, L.J. et al. (2002) The effect of dissolved oxygen (DO) concentration on the glycosylation of recombinant protein produced by the insect cell-baculovirus expression system. Biotechnology and Bioengineering, 11 (2), 219-224. 

Kang, C.Y. (1997) Expression of human immunodeficiency virus genes using baculovirus expression system. Molecular Biotechnology, 8 (2), 173-187. 

Berger, I., Fitzgerald, D.J. and Richmond, T.J. (2004) Baculovirus expression system for heterologous multiprotein complexes. Nature Biotechnology, 22 (12), 1583-1587. 

Bacteriological sulfur, 23 577-578 Bacteriophages, 3 135 12 474 in fermentation, 11 46 Bacteriorhodopsin, 20 826, 840 photochromic material, 6 603 Bacteriosins, 12 76. See also Bacteriocins Bacteriostatic water, 18 714 Bacterium lactis, 11 7 Baculovirus expression system, 5 346 Baddeleyite, 21 489 26 623-624 colorants for ceramics, 7 346t Badische Anilin und Soda Fabrik (BASF) terpenoid manufacture process, 24 481 Baeyer-Villiger oxidation reactions, 14 592 chiral recognition by enzymes, 3 675 microbial, 16 401 Baffled shellside flow, 13 262 Baffles, in stirred tank geometries,... 

Baculovirus expression is the most frequently used method for expression in insect cells and employs Autographa californica nuclear polyhedrosis virus (AcNPV), a double stranded (ds) DNA virus that infects arthropods. The baculovirus expression system utilizes features of the viral life cycle to introduce recombinant DNA coding the gene of interest into insect cells (Miller, 1988 O Reilly et al, 1992). 

Note. Protocols 1.5 to 1.11 have been adapted from Baculovirus Expression System Manual, 6th edn. May 1999 (WWW. bdbiosciences.com). 

King LA, Possee RD (1992) The Baculovirus Expression System a laboratory guide. Chapman Hall, London... 

Porcilis pesti (vaccine containing Intervet r classical swine fever virus E2 subunit antigen, produced in an insect cell baculovirus expression system)... 

In reverse chemical genetics, it is crucial to synthesize proteins of interest using appropriate foreign gene expression systems and cDNA resources. Since cell-free protein synthesis systems have the potential to synthesize any desired proteins, including both native proteins and those that are toxic to cells (1), with high throughput, they can be powerful tools for this objective. We developed a cell-free protein synthesis system from Spodop-tera fm iperda 21 (S 21) insect cells, which are widely used as the host for baculovirus expression systems, and commercialized it as the Transdirect insect cell. 

Kim, C.M. Dion, S.B. Onorato, J.J. Benovic, J.L. Expression and characterization of two -adrenergic receptor kinase isoforms using the baculovirus expression system. Receptor, 3, 39-55 (1993)... 

M.D. Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 p protein by using the baculovirus expression system. Biochem. J., 288, 395-405 (1992)... 

Jayakumar A, Kang Y, Mitsudo K, et al. Expression of LEKTI domains 6-9 in the baculovirus expression system Recombinant LEKTI domains 6-9 inhibit trypsin and subtilisin A. Protein Expr Purif 2004 35 93-101. 

Luckow VA (1995), Protein production and processing from baculovirus expression vectors, In Shuler ML, Wood HA, Granados RR, Hammer DA (Eds), Baculovirus Expression Systems and Biopesticides, J Wiley Sons, New York, pp. 51-90. 

A second milestone in the production of viral antigens was the development of the technology associated with insect cells - the baculovirus expression system. The Autographa californica baculovirus produces viral particles as a part of its life cycle. These particles accumulate in the polyhedrin protein matrix, which confers protection from inactivation due to environmental factors. The polyhedrin is produced in large amounts... 

LLu YC, Bentley WE (1999), Enhancing yield of infectious Bursal disease virus structural proteins in baculovirus expression systems focus on media, protease inhibitors, and dissolved oxygen, Biotechnol Prog. 15 1065-1071. 

Lanford RE, Luckow V, Kennedy RC, Dreesman GR, Notvall L, Summers MD (1989), Expression and characterization of hepatitis B virus surface antigen polypeptides in insect cells with a baculovirus expression system, J. Virol. 63 1549-1557. 

Nguyen B, Jarnagin K, Williams S, Chan H, Barnett J (1993), Fed-batch culture of insect cells a method to increase the yield of recombinant human nerve growth factor (rhNGF) in the baculovirus expression system, J. Biotechnol. 31 205-217. 

Osman AA, Richter T, Stern M, Conrad K, Henker J, Brandsch C, et al. Production of recombinant human tissue transglutaminase using baculovirus expression system and its application for serologic diagnosis of coeliac disease. Eur J Gastroenterol Hepatol 2002 14 1217-1223. 

The baculovirus expression vector system (BEVS) is now widely used for recombinant protein production [23]. Unfortunately, this system suffers from low yields and high production costs, and therefore is inappropriate for industrial scaling-up. Several peroxidases have been successfully produced on a small scale (Table 12.3) using the baculovirus expression system, such as MnP isozyme H4 and LiP isozymes H2 and H8 [17, 24, 25],... 

Hitchman RB, Possee RD, King LA (2009) Baculovirus expression systems for recombinant protein production in insect cells. Recent Pat Biotechnol 3 46—54... 

Recombinant baculoviruses have been used as vectors to express heterologous proteins in cultured insect cells (3). A number of unique features distinguish the baculovirus expression system from other expression systems ... 

Bac-to-Bac Baculovirus Expression System (Gibco-BRL) plasmid pFastBacl, MAX Efficiency DHlOBac competent cells, and CellFECTIN reagent. 

Heseman, C. A., and Capra, D. J. (1990). High level production of a functional immunoglobulin heterodimer in a baculovirus expression system. Proc. Natl. Acad. Sci. U.S.A. 87, 3942-3946. 

Glaser, V. (1993). Biotechnology industry s interest grows in baculovirus expression systems. Genetic Engineering News 13, March 15, 10 11. 

---