Heterologous protein expression

Cereghino, J.L. and Cregg, J.M. (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiology Reviews, 24 (1), 45-66. 

Madzak, C., Gaillardin, C. and Beckerich, J.M. (2004) Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica. a review. Journal of Biotechnology, 109 (1—2), 63—81. 

The vast bulk of proteins synthesized naturally by E. coli (i.e. its homologous proteins) are intracellular. Few are exported to the periplasmic space or released as true extracellular proteins. Heterologous proteins expressed in E. coli thus invariably accumulate in the cell cytoplasm. Intracellular protein production complicates downstream processing (relative to extracellular production) as ... 

Hannig, G., Makrides, S. C. Strategies for optimizing heterologous protein expression in Eschericia coli. TIBTECH 16 54-60 (1998). 

Bottlenecks for Heterologous Protein Expression in Filamentous Fungi. 324... 

Two basic strategies have been employed for heterologous protein expression in plants. The first is the creation of transgenic plants in which the foreign gene is stably incorporated into the plant genome, transcribed through the nuclear apparatus of the plant, and inherited by the next... 

Lin Cereghino J L, Cregg J M (2000). Heterologous protein expression in the methy-lotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24 45-66. 

Tate C G, Haase J, Baker C, Boorsma M, Magnani E, Vallis Y, Williams D C (2003). Comparison of seven different heterologous protein expression systems for the production of the serotonin transporter. Biochim. Biophys. Acta. 1610 141-153. 

D. P., and Komives, E.A. (1998) Generation of protease-deficient strains and their use in heterologous protein expression. Methods Mol Biol, 103, 81-94. 

Gustafsson, C., Govindarajan, S., and Minshull,). (2004) Codon bias and heterologous protein expression. Trends Biotechnol, 22 (7), 346-353. 

Nevalainen KM, Te o VS, Bergquist PL (2005) Heterologous protein expression in filamentous fungi. Trends Biotechnol 23(9) 468-474... 

For heterologous protein expression, a cloning vector must contain at least a functional origin of replication (on) suitable for the host microorganism, one or more selection marka-s such as the antibiotic resistance gene or the wild-type gene for auxotrophic bacterial strains, and a multiple cloning site. 

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