Polypeptide as antigens

The cAPP polypeptide APP(724-770) was also used as antigen for elucidation of the epitope recognisedby a monoclonal anti-cAPP antibody (Jonas-mAb), which has been... 

In this study, a number of C-terminal APP polypeptides were synthesised and efficiently employed as antigens in the structural determination of epitopes recognised by monoclonal and polyclonal antibodies specific for the Carboxy-terminal, intracellular domain of APP, using proteolytic epitope-excision and -extraction in combination with high resolution FTICR-mass spectrometry. The results of these experiments specifically define the epitope of the cAPP polyclonal antibody (36BO) as the sequence, APP(727-737), while the epitope recognised by the Jonas-mAb was defined at the... 

With respect to the advantage of using a protein rather than a synthetic polypeptide as a carrier, we can refer to Jaffe et who showed that an active fragment of gastrin, its C-terminal tetrapeptide amide, was antigenic when covalently attached to serum protein carriers but not when linked to poly(L-lysine) or to poly(L-glutamic acid). Walker et al. in 1973 made similar comparisons with steroid conjugates and obtained the same result, i.e., bovine serum albumin was a better carrier than poly(L-lysine). (But also see below. 

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