Infectious bursal disease virus

Infectious bursal disease virus (IBVD) primarily infects poultry. VP2 of IBVD has been expressed in transgenic Arabidopsis. While the serum antibody response in chickens fed with leaf extracts worked less efficiently than commercial vaccine (60% versus 90%) it worked just as efficiently (80%) when used in a booster format along with the commercial vaccine (Wu et al., 2004, 2007). In addition to this, tobacco cell cultures have been 

Biopharmaceuticals in Plants Toward the Next Century of Medicine 

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DASL, cDNA-mediated annealing, selection, extension, and ligation GAPDH, glyceraldehydes-3-phosphate dehydrogenase IBDV, infectious bursal disease virus VEGF, vascular endothelial growth factor. 

Hamoud MM, Villegas P, Williams SM. Detection of infectious bursal disease virus from formalin-fixed paraffin-embedded tissue by immunohistochemistry and realtime reverse transcription-polymerase chain reaction. J. Vet. Diagn. Invest. 2007 19 35—42. 

Infection-responsive drug delivery, 9 63 Infections, sulfonamides for, 23 499 Infectious Bursal Disease Virus (IBDV) vaccine, from yeast, 26 487-488 Infectious diseases... 

Wu, H., Singh, N.K., Locy, R.D., Scissum-Gunn, K., and Giambrone, JJ. (2004). Immunization of chickens with VP2 protein of infectious bursal disease virus expressed in Arabidopsis thaliana. Avian Dis. 48 663-668. 

Wu, J., Yu, L., Long, L., Hu, J., Zhou, J., and Zhou, X. (2007). Oral immunization with transgenic rice seeds expressing VP2 protein of infectious bursal disease virus induces protective immune responses in chickens. Plant Biotechnol. J. 5(5) 570-578. 

LLu YC, Bentley WE (1999), Enhancing yield of infectious Bursal disease virus structural proteins in baculovirus expression systems focus on media, protease inhibitors, and dissolved oxygen, Biotechnol Prog. 15 1065-1071. 

Wang MY, Kuo YY, Lee MS, Doong SR, Ho JY, Lee LH (2000), Self-assembly of the infectious bursal disease virus capsid protein, rVP2, expressed in insect cells and purification of immunogenic chimeric rVP2H particles by immobilized metal-ion affinity chromatography, Biotechnol. Bioeng. 67 104-111. 

Bottcher, B., Kiselev, N. A., Stel Mashchuk, V. Y., Perevozchikova, N. A., Borisov, A. V., and Crowther, R. A. (1997a). Three-dimensional structure of infectious bursal disease virus determined by electron cryomicroscopy. / Virol. 71, 325-330. 

The immunochromatographic lateral flow strip test is a one-step test that facilitates low-cost, rapid identification of various analytes at the point of care. We have developed lateral flow strip tests for the specific qualitative or semiquantitative detection of antigens, antibodies, and haptens, such as drug residues. Here, we describe in detail the preparation of three examples of the strip tests for detection of (a) the infectious bursal disease virus (b) Tricbinella specific antibodies, and (c) Clenbuterol residues in urine samples. 

Key words Immunochromatography, Lateral flow strip test, Infectious bursal disease virus, Tricbinella, Clenbuterol. 

Infectious Bursal Disease Virus scFv Chicken Immune [92]... 

Sapats S I, Heine H G, Trinidad L, et al. (2003). Generation of chicken single chain antibody variable fragments (scFv) that differentiate and neutralize infectious bursal disease virus (IBDV). Arch. Virol. 148 497-515. 

Sun J H, Yan Y X, Jiang J, et al. (2005). DNA immunization against very virulent infectious bursal disease virus with VP2-4-3 gene and chicken IL-6 gene. J. Vet. Med. B Infect. Dis. Vet. Public. Health. 52 1-7. 

Hu Y.C., Bentley W.E., Edwards G.H. and Vakharia V.N. 1999. Chimeric infectious bursal disease virus-like particles expressed in insect cells and purified by immobilized metal affinity chromatography, Biotechnol. Bioeng., 63, 721. 

Intimate mixture under pressure of the polymer material with a core material before or after SCF solvation of the polymer, followed by an abrupt release of pressure, leads to an efficient solidification of the polymeric material around the core material. This technique was used to microencapsulate infectious bursal disease virus vaccine in a polycaprolactone (PCL) or a poly(lactic-co-glycolic acid) (PLGA) matrix " ]. 

For the first stage of such study, we have followed the effect of polysaccharide-containing extracellular fractions (EF) from the edible mushroom P. ostreatus on broiler chickens, which have not been vaccinated with BIAVAC and BIAROMVAC vaccines (Figure 3.20). In this case the maternal titers of infectious bursal disease virus antibodies (IBD-AB) (control) are seen to decrease, being at the minimum level (96) in four weeks post hatching (Figure 3.20a). This antibody titer (96) was below the estimated cut-off level in this enz5mie-linked immunosorbent assay (ELISA) system. 

Selegean, M., Putz, M. V., Rugea, T. (2009). Effect of the polysaccharide extract from the edible mushroom Pleurotus Ostreatus against infectious bursal disease virus. Int J. MolSci. 10(8), 3616-3634 (DOI 10.3390/ijmsl 0083616). 

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