Expression vectors baculovirus

Philipps, B., Forstner, M. and Mayr, L.M. (2005) A baculovirus expression vector system for simultaneous protein expression in insect and mammalian cells. Biotechnology Progress, 21 (3), 708-711. 

Insect cells in culture are also hosts for recombinant protein production. Production of recombinant proteins in the baculovirus expression vector system is the most common system. Titers of recombinant protein as high as 11 g/L have been obtained. 

Kitts, R A. and Possee, R. D. (1993). A method for producing recombinant baculovirus expression vectors at high frequency. Biotechniques 14,810-817. 

Smith, G. E., Summers, M. D., and Fraser, M. J. (1983). Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol. Cell Biol. 3, 2156-2165. 

Baculovirus Expression Vector System Manual, 6th edition May 1999 (www. bdbiosciences.com). 

O Reilly DR, Miller LK (1989) Baculovirus expression vectors, a laboratory manual. WH Freeman and Co., New York... 

Smith, G.E., Ju, G., Ericson, B.L., Moschera, J., Lahm, H.W., Chizzonite, R. Summers, M.D. (1985). Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proceedings of the National Academy of Sciences (USA) 82, 8404-8. 

It is worth emphasizing that all biopharmaceuticals mentioned here are produced from mammalian cell culture. The protein production system based on insect cells known as BEVS (baculovirus expression vector system) is widely employed for the expression of a wide range of proteins, but, due to regulatory issues, biopharmaceuticals produced by insect cells are not yet in the market. However, some of them are being evaluated,... 

Luckow VA (1995), Protein production and processing from baculovirus expression vectors, In Shuler ML, Wood HA, Granados RR, Hammer DA (Eds), Baculovirus Expression Systems and Biopesticides, J Wiley Sons, New York, pp. 51-90. 

Jarvis DL, Finn E (1996), Modifying the insect cell N-glycosylation pathway with immediate early baculovirus expression vectors, Nat. Biotechnol. 14 1288-1292. 

The values of qp can vary significantly as a function of the expression system used and of the recombinant protein product. In general, values in the range of 1 —30 pg cell-1 day-1 are reported for stably transfected cell lines. However, Chico and Jager (2000) have reported values as high as 85 pg cell-1 day-1 for the expression of a protein using the insect cell-baculovirus expression vector system. This value approaches the maximum theoretical value that is expected for animal cells, which is 100 pg cell-1 day-1 (Ozturk, 1990). 

Roy P (1990), Use of baculovirus expression vectors development of diagnostic reagents, vaccines and morphological counterparts of bluetongue virus, FEMS Microbiol. Immunol. 2 223-234. 

Jarvis AW (1997), Baculovirus expression vectors. In Miller LK (Ed.), The Baculoviruses, Plenum Press, New York, pp. 389-431. 

ADA BEVS BHK CHO ELISA mAb MDCK MMR SCID tPA VLP adenosine deaminase deficiency baculovirus expression vector system baby hamster kidney cell line Chinese hamster ovary cell line enzyme linked immuno sorbent assay monoclonal antibodies Madin-Darby canine kidney epithelial cells measles, mumps, rubella severe combined immunodeficiency plasminogen activator virus-like particle... 

J. Vlak and R. Keus, "Baculovirus Expression Vector System for Production of Viral Vaccines," in A. Mizrahi, ed., Viral Vaccines, Wiley- Liss, New York, 1990. 

The baculovirus expression vector system (BEVS) is now widely used for recombinant protein production [23]. Unfortunately, this system suffers from low yields and high production costs, and therefore is inappropriate for industrial scaling-up. Several peroxidases have been successfully produced on a small scale (Table 12.3) using the baculovirus expression system, such as MnP isozyme H4 and LiP isozymes H2 and H8 [17, 24, 25],... 

Licari, P. J., Jarvis, D. L., and Bailey, J. E. (1993). Insect cell hosts for baculovirus expression vectors contain endogenous exoglycosidase activity. Biotechnol. Prog. 9, 146-152. 

Knecht, W., Bergjohann, U., Gonski, S., Kirschbaum, B., Loefeler, M. (1996). Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified recombinant enzyme. 

Luckow VA Summers MD (1988) Trends in the development of baculovirus expression vectors (review). Biotechnology 6 47-55. 

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