Expression of Foreign Genes

Mitsuhara, 1., Ugaki, M., Hirochika, H., Ohshima, M., Murakami, T., Gotoh, Y., Katayose, Y, Nakamura, S., Honkura, R Nishimiya, S., Ueno, K., Mochizuki, A., Tanimoto, H., Tsugawa, H., Otsuki, Y, and Ohashi, Y. (1996). Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants. Plant Cell Physiol. 37(1) 49-59. 

Toth, R.L., Chapman, S., Carr, E, and Santa Cruz, S. (2001). A novel strategy for the expression of foreign genes from plant virus vectors. FEBS Lett. 489 215-219. 

De Beilis, D. and Schwartz, I. (1990) Regulated expression of foreign genes fused to lac-control by glucose levels in growth medium. Nucleic Acids Res 18, 1311... 

Y. Miyao, K. Shimizu, S. Moriuchi, M. Yamada, K. Nakahira, K. Nakajima, J. Nakao, S. Kuriyama, T. Tsujii, K. Mikoshiba, et al., Selective expression of foreign genes in glioma cells use of the mouse myelin basic protein gene promoter to direct toxic gene expression, J. Neurosci. Res. 36 412 (1993). 

Laparra, H., Burrus, M., Hunold, R., Damm, B., Bravo-Angel, A.-M., Bronner, R., and Hahne, G., Expression of foreign genes in sunflower (Helianthus annuus L.) evaluation of three gene transfer methods, Euphytica, 85, 63-74, 1995. 

Batra LR Taxonomy and systematics of the Hemiascomycetes (Hemiascomycetidae) in Subramanian CV (ed) Taxonomy of Fungi. Madras, University of Madras, 1973, pp 187-214. Cregg JM, Vedvick TS, Raschke WC Recent advances in the expression of foreign genes in Pichiapastoris. Biotechnology 1993 11 905-910. 

Vaccinia virus is a large double-stranded DNA virus that infects many types of mammalian cells and some invertebrate cells. Its replication cycle is confined to the cytoplasm and can be divided into an early phase and a late phase. The late phase is characterized by the synthesis of structural proteins and enzymes that are packaged into progeny virions. Expression of foreign genes from recombinant vaccinia viruses takes advantage of promoters that are active during the late phase in order to maximize yields of the protein of interest. Several features make the vaccinia virus system... 

Cheng Q, Aksoy S. Tissue tropism, transmission and expression of foreign genes in vivo in midgut symbionts of tsetse flies. Insect Mol. Biol. 1999 8 125-132. 

Expression of Foreign Genes in Mammalian Cells Using an Antibody Fusion System... 

Kaufman, R. J., Davies, M. V., Wasley, L. C. and Michnick, D. (1991). Improved vectors for stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virus. Nucleic Acids Res. 19, 4485-4490. 

Choudary, P.V., Kamita, S.G. and Maeda, S. Expression of foreign genes in Bombyx mori larvae using baculovirus vectors. Methods Mol. Biol, 39, 243, 1995. 

Kim, S.Y., Lee, J.H., Shin, H.S., Kang, H.J., and Kim, Y. S. (2002) The human elongation factor 1 alpha (EF-1 alpha) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter. J. Bio-technol. 93 183-187. 

Microbial enzymes produced by fermentation under controlled conditions constitute now the most relevant option for enzyme synthesis. Microbial strains can produce not only the enzyme proteins coded by their own genetic information, but also those produced by the expression of foreign genes as recombinant proteins. As analyzed before, microorganisms are ideal hosts for enzyme synthesis and only those glycoenzymes which cannot be properly produced in microbial hosts are to be produced in plant (Ma et al. 2003) or animal cell culture (Altamirano et al. 2004 Wurm 2004). 

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