Baculovirus expression

Bryant Yes, although we didn t use purified IDGF, but recombinant IDGF produced from a baculovirus expression system. These are clone 8 cells from an imaginal disc we haven t tried growing the whole imaginal disc. 

Zhang, F., Saarinen, M.A., Itle, L.J. et al. (2002) The effect of dissolved oxygen (DO) concentration on the glycosylation of recombinant protein produced by the insect cell-baculovirus expression system. Biotechnology and Bioengineering, 11 (2), 219-224. 

Philipps, B., Forstner, M. and Mayr, L.M. (2005) A baculovirus expression vector system for simultaneous protein expression in insect and mammalian cells. Biotechnology Progress, 21 (3), 708-711. 

Kang, C.Y. (1997) Expression of human immunodeficiency virus genes using baculovirus expression system. Molecular Biotechnology, 8 (2), 173-187. 

Assenga, S.P, You, M., Shy, C.H. et al. (2006) The use of a recombinant baculovirus expressing a chitinase from the hard tick Haemaphysalis longicornis and its potential application as a bioacaricide for tick control. Parasitology Research, 98 (2), 111-118. 

Berger, I., Fitzgerald, D.J. and Richmond, T.J. (2004) Baculovirus expression system for heterologous multiprotein complexes. Nature Biotechnology, 22 (12), 1583-1587. 

Veijola, J., Koivunen, P., Annunen, P., Pihlajaniemi, T. and Kivirikko, K. (1994) Cloning, baculovirus expression, and characterization of the alpha-subunit of prolyl 4-hydroxylase from the nematode Caenorhabditis elegans - this alpha-subunit forms an active alpha-beta dimer with the human protein disulfide-isomerase beta-subunit. Journal of Biological Chemistry 269, 26746-26753. 

Novelli, A., and Boulanger, P. A. (1991). Deletion analysis of functional domains in baculovirus-expressed adenovirus type 2 fiber. Virology 185, 365-376. 

Bacteriological sulfur, 23 577-578 Bacteriophages, 3 135 12 474 in fermentation, 11 46 Bacteriorhodopsin, 20 826, 840 photochromic material, 6 603 Bacteriosins, 12 76. See also Bacteriocins Bacteriostatic water, 18 714 Bacterium lactis, 11 7 Baculovirus expression system, 5 346 Baddeleyite, 21 489 26 623-624 colorants for ceramics, 7 346t Badische Anilin und Soda Fabrik (BASF) terpenoid manufacture process, 24 481 Baeyer-Villiger oxidation reactions, 14 592 chiral recognition by enzymes, 3 675 microbial, 16 401 Baffled shellside flow, 13 262 Baffles, in stirred tank geometries,... 

Insect cells in culture are also hosts for recombinant protein production. Production of recombinant proteins in the baculovirus expression vector system is the most common system. Titers of recombinant protein as high as 11 g/L have been obtained. 

Baculovirus expression is the most frequently used method for expression in insect cells and employs Autographa californica nuclear polyhedrosis virus (AcNPV), a double stranded (ds) DNA virus that infects arthropods. The baculovirus expression system utilizes features of the viral life cycle to introduce recombinant DNA coding the gene of interest into insect cells (Miller, 1988 O Reilly et al, 1992). 

Note. Protocols 1.5 to 1.11 have been adapted from Baculovirus Expression System Manual, 6th edn. May 1999 (WWW. bdbiosciences.com). 

Kitts, R A. and Possee, R. D. (1993). A method for producing recombinant baculovirus expression vectors at high frequency. Biotechniques 14,810-817. 

Smith, G. E., Summers, M. D., and Fraser, M. J. (1983). Production of human beta interferon in insect cells infected with a baculovirus expression vector. Mol. Cell Biol. 3, 2156-2165. 

O Reilly DR, Miller LK (1989) Baculovirus expression vectors, a laboratory manual. WH Freeman and Co., New York... 

King LA, Possee RD (1992) The Baculovirus Expression System a laboratory guide. Chapman Hall, London... 

Porcilis pesti (vaccine containing Intervet r classical swine fever virus E2 subunit antigen, produced in an insect cell baculovirus expression system)... 

In reverse chemical genetics, it is crucial to synthesize proteins of interest using appropriate foreign gene expression systems and cDNA resources. Since cell-free protein synthesis systems have the potential to synthesize any desired proteins, including both native proteins and those that are toxic to cells (1), with high throughput, they can be powerful tools for this objective. We developed a cell-free protein synthesis system from Spodop-tera fm iperda 21 (S 21) insect cells, which are widely used as the host for baculovirus expression systems, and commercialized it as the Transdirect insect cell. 

Kim, C.M. Dion, S.B. Onorato, J.J. Benovic, J.L. Expression and characterization of two -adrenergic receptor kinase isoforms using the baculovirus expression system. Receptor, 3, 39-55 (1993)... 

M.D. Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 p protein by using the baculovirus expression system. Biochem. J., 288, 395-405 (1992)... 

Hota-Mitchell, S., Siddiqui, A.A., Dekaban, C.A., Smith, J., Tognon, C. and Podesta, R.B. (1997) Protection against Schistosoma mansoni infection with a recombinant baculovirus-expressed subunit of calpain. Vaccine 15, 1631-1640. 

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