AE-cellulose

The absorption spectra of the fractions obtained during the purification of the PSI particles are shown in Fig la. Both the sonicate and the original 40,000 xg supernatant showed a major peak at 620 nm due to soluble phycobiliproteins, which can be completely removed by batch chromatography on DE AE-cellulose. Thus, the A620/A676 ratio, which compares the absorption maxima of blue pigments to that of chlorophyll was 2.73 in the sonicate, 3.81 in the 40,000 xg supernatant, and only 0.27 in the DE-52 eluate. 

Trolit AE Cellulose ether Dynamit Nobel, Germany... 

Fig. 5. Effect of HCOj -free medium on inactivation and phosphorylation of acetyl-CoA carboxylase. Enzyme preparation was placed in the main compartment of a Warburg flask, capped with a serum stopper, containing a small piece of Whatman filter paper (2x3 cm) plus 0.1 ml of Hyamine 10-X in the central well. Vessels were evacuated and refilled with COj-free Nj several times. This procedure was repeated several times during a 3-hour period at room temperature to completely remove dissolved HCOs firom the medium. Enzjmie preparation was equilibrated at 37°C for 10 minutes prior to the addition of [y- PlATP (specific activity, 28 iiCi/iimole at 0.7 miW final concentration) which was also treated in a similar way to remove COi. AT indicated times, P incorporation was terminated by addition of a cold solution containing ATP and EDTA at 5 times the concentrations of [y- PlATP and MgCl2, respectively. The labeled en me was purified by DE AE-cellulose chromatography as described before, and immunoprecipitation of the enzyme was carried out by incubation with rabbit antibody to the carboxylase for 15 minutes and with the membrane preparation fromS. aureus (18) for another 15 minutes. Enzyme-antibody precipitates were collected and immediately washed by centrifugation. Enzyme activity-control enzyme -ATP, O +ATP, . COj-free enzyme —ATP, A -H ATP, . Radioactivily-control enzyme, C02-free enzyme, . From Lent et al. (71).

We have attempted to dissect the incorporation of NAD into these two reactions by snake venom phosphodiesterase (SVPDE) digestion. The rate of incorporation of label into 5 -AMP gives an estimate of the rate of initiation and the rate of incorporation into phosphoribosyl-AMP(PR-AMP) estimates the rate of elongation. AE-cellulose chromatography can be used to monitor the proportion of 5 -AMP deriving from mono- and poly(ADP-ribose) residues respectively. This provides an important check since, in permeabilised cells, mono(ADP-ribose) residues can be produced other than in the nucleus and also by non-enzymic reactions [9],... 

Poly(2-methoxypropyl, phthaloyl) [9050-31-1] Coating for controlled release of pharmaceutical formulations. Poly-0-(2-aminoethyl) 2-Aminoethylcel-lulose. AE-cellulose [9032-36-4] Substrate for immobilised enzymes in affinity chromatography. Poly-0-(2-diethylaminoethyl) DEAE-cellulose... 

Adonitol, R-94 Adonose, P-47 eADP, R-110 ADP, A-33 ADPG, A-36 ADPR, A-37 Adriacin, A-58 Adriamycin, A-58 Adriamycinol, A-58 Adriamycinone, A-58 Adriblastin, A-58 AE-cellulose, C-48 Aediirid, E-2 Aerosporin, S-86 AF, A-879... 

Table 2. Swelling of polyacrylamide hydrogels crosslinked with allyl ether of carboxymethyl cellulose (AE CMC)1 [41]...

Heinz body preparations are positive as Is the heat stability test Electrophoretic examination of hemolysate showed the presence of a fast-moving variant which was readily separated from Hb-A by chromatography on DEAE-Sephadex The abnormal 3- chain was Isolated by CM-Cellulose chromatography as described before, and converted into the S-2-amlnoethyl (AE) derivative... 

Urine Extraction of sample with polydithio-carbamate resin and NaOH filtration on cellulose ester membrane neutralization with NaOH ashing dissolution and heating dilution with distilled water ICP/AES (Method P CAM 8310) 0.005 pg/mL 100 NIOSH 1984... 

Air (particulate lead) Collection of particulate matter onto cellulose acetate membrane filter wet ashing with HN03 / HCI04 ICP/AES (Method P CAM 7300) 5 pg/m3 95-105 NIOSH 1984... 

In various runs, specific activity went from an average of 2.75 lU/mg protein (based on the Bio-Rad protein assay with bovine serum albumin standard) for Ae centrifuged crude preparation after alcohol precipitation, to 8 lU/mg after DEAE-cellulose chromatography, to 100 lU/mg after DEAE-Fractogel chromatography, to 1000 RJ/mg after Sephadex G-50 chromatography, and to 3200 lU/mg after CM-Trisacryl chromatography. 

Specificity. Under Ae conAtions of the assay, Xylanase II had little or no activity on cellulose, carboxymeAylcellulose, Avicel, and starch. 

Air, airborne particulates Collection on cellulose acetate filter digestion with concentrated nitrated and perchloric acids ICP-AES, NIOSH 7300 1 Mg/ sample 105% at 2.5 Mg 97% at 1 mg NIOSH 1994b... 

Weigh exactly 20 ae 1 g of sample into cellulose extraction thimbles. Cover the top of each thimble with glass wool to prevent floating. 

ABA ABS ABS-PC ABS-PVC ACM ACS AES AMMA AN APET APP ASA BR BS CA CAB CAP CN CP CPE CPET CPP CPVC CR CTA DAM DAP DMT ECTFE EEA EMA EMAA EMAC EMPP EnBA EP EPM ESI EVA(C) EVOH FEP HDI HDPE HIPS HMDI IPI LDPE LLDPE MBS Acrylonitrile-butadiene-acrylate Acrylonitrile-butadiene-styrene copolymer Acrylonitrile-butadiene-styrene-polycarbonate alloy Acrylonitrile-butadiene-styrene-poly(vinyl chloride) alloy Acrylic acid ester rubber Acrylonitrile-chlorinated pe-styrene Acrylonitrile-ethylene-propylene-styrene Acrylonitrile-methyl methacrylate Acrylonitrile Amorphous polyethylene terephthalate Atactic polypropylene Acrylic-styrene-acrylonitrile Butadiene rubber Butadiene styrene rubber Cellulose acetate Cellulose acetate-butyrate Cellulose acetate-propionate Cellulose nitrate Cellulose propionate Chlorinated polyethylene Crystalline polyethylene terephthalate Cast polypropylene Chlorinated polyvinyl chloride Chloroprene rubber Cellulose triacetate Diallyl maleate Diallyl phthalate Terephthalic acid, dimethyl ester Ethylene-chlorotrifluoroethylene copolymer Ethylene-ethyl acrylate Ethylene-methyl acrylate Ethylene methacrylic acid Ethylene-methyl acrylate copolymer Elastomer modified polypropylene Ethylene normal butyl acrylate Epoxy resin, also ethylene-propylene Ethylene-propylene rubber Ethylene-styrene copolymers Polyethylene-vinyl acetate Polyethylene-vinyl alcohol copolymers Fluorinated ethylene-propylene copolymers Hexamethylene diisocyanate High-density polyethylene High-impact polystyrene Diisocyanato dicyclohexylmethane Isophorone diisocyanate Low-density polyethylene Linear low-density polyethylene Methacrylate-butadiene-styrene... 

Air Collect sample on cellulose filter and digest with nitric acid Method 7300 (ICP-AES) 1 g/sample NA NIOSH 1984b... 

Table II. Color Difference (AE), Standard Deviation (SD) and Grey Scale for Staining Rating (GSS) of Naturally and Artificially Aged Cellulosic Fabrics Immediately After Deacidification1,2, 3... 

Air (total chromium) Particulate matter collected on cellulose ester filter, digested with aqua regia ICP-AES 1 pg/m3 87-102% at 0.5-100 pg Lo and Arai 1988... 

Air (total chromium) Sample collected on cellulose ester membrane filter dissolved in acid mixtures ICP-AES 1 pg/sample 98% at 2.5 pg/filter NIOSH 1994d (Method 7300)... 

Cellulose acetate films of the same thickness (8 mil) containing five different stabilizers at three concentrations were evaluated for their effectiveness in protecting the blue wool fabrics (Table II). Unprotected wool fabric and wool fabric covered with film containing no stabilizers exposed to the xenon-arc source under comparable conditions served as primary and secondary controls. Film without any stabilizer offered little protection throughout the exposure period after 550 kj/m2 exposure, fabric protected by such a film had a AE value of 2.38 and unprotected fabric had a value of 2.70. 

The other commercial stabilizer (UV-3), a hindered-amine type, is presumed to afford photochemical protection by functioning primarily as a free radical and/or oxygen scavenger (13). Thus protection should only occur in the primary substrate, the cellulose acetate film, and not in a secondary substrate such as the blue wool fabric. The results shown in Table II verified this assumption the AE values obtained, irrespective of the concentration of UV-3 used in the film, were within experimental error of those observed with the unprotected fabric. 

Figure 1. Least-squares fit to exponential curve y = abx (y = AE x = kj/m2) for unprotected blue wool fabric, and for blue wool fabrics covered with cellulose acetate films (8-mil) containing no stabilizer, or one or more stabilizers at 0.5% concentration...

Figure 3. Plot of AE after 550 kj/m2 exposure of blue wool fabric with and without cellulose acetate films of varying thickness, films contained stabilizers at 0.5% concentration...

Cellulose ion eselnmgers Diethylaminocihyl cellulose (DEAE-) Triethylaminocthyl cellulose (TEAE-) Quaternary aminoethyl cellulose (QAE-l Aminoethyl cellulose (AE-)... 

Several functional groups have been used to obtain cellulose anion exchangers [aminoethyl (AE), diethyl-aminoethyl (DEAE)], or cation exchangers [car-boxymethyl (CM), phosphate (P)] for thin-layer chromatography. PEI cellulose is not a chemically modified cellulose, but a complex of cellulose with polyethyleneimine. These cellulose exchangers are particularly useful for the separation of proteins, aminoacids, enzymes, nucleobases, nucleosides, nucleotides, and nucleic acids. 

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