Aminoethyl cellulose

More efficient in producing 2-aminoethylated cellulose. Relatively inexpensive and doesn t require organic solvent... 

Figure 19.9. Chromatogram for stepwise elution of bovine serum albumin on a Vistec diethyl aminoethyl cellulose ion-exchanger, using stepwise increases in sodium chloride concentration in the mobile phase to achieve selective desorption. Proteins 1, serum fraction not adsorbed by column (includes y-gobuhn) 2,3,...

Many types of derivatives of cellulose have been used to prepare affinity adsorbents.51 0-(2-Aminoethyl)cellulose and 0-(carboxy-methyl)cellulose can be coupled to carbohydrates bearing carboxyl or primary amino groups by the carbodiimide method.52,53 Cellulose derivatives bearing amino groups can be coupled to reducing carbohydrates by reductive-amination reactions25 in which the Schiff base produced in the initial reaction is reduced with sodium cyanoborohy-dride.54... 

Aminoethylcel lulose(AEC) (Poly aminoethylated Cellulose). The aminoethylation of cellulose with ethyleneimine had been studied prior to WW II but not for the purpose of using the product as a propellant. Most of the aminoethylated... 

Refs 1) Beil, not found 2) C.T. Lenk et al, "Studies on the Preparation of Aminoethyl Cellulose Perchlorate and Other Fast Burning Propellant s , Summary Rept 3, Wyandotte Chemicals Corp (1957), 18—19... 

Kaul and Lester (10) reported the preparation of six novel glycophosphoceramide fractions from the above crude concentrate from tobacco leaves. The crude concentrate was first resolved into two groups by column chromatography on diethyl aminoethyl-cellulose. The first group contained no acetyl residues, whereas the second group contained one N-acetyl per phosphorus. Three lipid fractions from each group were further resolved by chromatography on Porasil columns. The chemical composition and the percent of the total P in the crude concentrate of these lipid frac-... 

The product was purified by chromatography on columns of DEAE (diethyl aminoethyl) cellulose (3 x 1) followed by CM (carboxymethyl) cellulose (6 x 1), developed with water. Nearly all the color washed quickly through DEAE cellulose. The effluent and washes were applied to the CM cellulose column, which was further developed with water. Elution was continued as long as this fraction continued to emerge, in a total of 850 ml. One half of this fraction (425 ml) was concentrated to a few ml under reduced pressure it crystallized slowly after adding acetone to slight turbidity. So cobamamide was obtained. 

Isolated inulinase is utilized for either batch hydrolysis of inulin or immobilization for flowthrough column hydrolysis systems. For example, purified inulinase from K. fragilis has been immobilized on 2-aminoethyl cellulose (Kim et al., 1982). 

Work has also been conducted on the removal of copper from milk. Thiosuccinylated aminoethyl cellulose has been used to remove more than 90% of the copper from milk (Roh et al., 1976). Glass-bound trypsin has been used to inhibit metal-induced lipid oxidation (Shipe et al., 1972). Further work by Gregory and Shipe (1975) showed that ageing milk before exposure to a metal catalyst reduced the extent of lipid oxidation and... 

Aminoethylcel lulose(AEC) (Polyaminoethyloted Cellulose). The aminoethylation of cellulose with ethyleneimine had been studied prior to WW 11 but not for the purpose of using the product as a propellant. Most of the aminoethylated celluloses prepd before work was undertaken by the BRL and the Wyandotte Chem Corp under contract with the Dept of the Army, Ordnance Corps(see Ref 1 under Aminoethylation Reactions) contained only a small amt of nitrogen. The material prepd at BRL contained up to 20% N(Refs 2 3) and the material prepd by the Wyandotte Corp contained 26+% N (Ref 4)... 

Crystalline enzymes have been obtained from rabbit, - " human,pigeon, and lobster skeletal muscle, rabbit heart, and potatoes, and phosphorylases from dog heart, and dog, pig, and rabbit liver have been extensively purified. Preparative procedures have usually involved ammonium sulfate fractionation and heat treatment, but adsorption on calcium phosphate, 0-(2-diethyl-aminoethyl)cellulose chromatography, - and electrophoresis on... 

Cellulose ion eselnmgers Diethylaminocihyl cellulose (DEAE-) Triethylaminocthyl cellulose (TEAE-) Quaternary aminoethyl cellulose (QAE-l Aminoethyl cellulose (AE-)... 

The utility of the 2-diethylaminoethyl ethers of insoluble polysaccharides is well established in the field of ion-exchange chromatography, and so such compounds as 0-( 2-diethylaminoethyl) cellulose and 0-(2-diethylaminoethyl) cross-linked dextran (DEAE-Sephadex) are common laboratory reagents for fractionations. The preparation, properties, and uses of 0-(2-diethylaminoethyl)-, 0-[2-(triethylammonium)-ethyl]-, and 0-(2-aminoethyl)-cellulose have been reviewed. Modification of the reaction conditions originally employed for diaminoethylation... 

Hydroxyethyl)methylamino] ethyl ether groups have been introduced into cellulose by modification with bis (2-chloroethyl) methyl-amine, and reaction of 0-(2-aminoethyl) cellulose with an aqueous solution of N-[3-(dihydroxyboryl) phenyl ]succinamic acid in the presence of a water-soluble carbodiimide yields 0- N- N-[3-(dihydroxy-boryl) phenyl ]succinamoyl)aminoethyl cellulose (10), which has a variety of applications in the separation of sugars, polyhydric alcohols, and nucleic acid components. ... 

Page 390, Table VII, column 1, entry 2 (under Amino derivative). For 0-(2-Di ethylaminoethyl) cellulose read 0-( 2-Aminoethyl) cellulose. ... 

Freeze-dried cells of M. neoaurum ATCC 25795 and partially purified amidase (amidase activity in cell extracts purified fivefold by diethyl aminoethyl cellulose column chromatography) were used for the biotransformation of compound (25). A reaction yield of 49 M% and an e.e. of 78% were obtained for the desired product (23) using freeze-dried cells. The reaction was completed in 50 h. Using partially purified amidase, a reaction yield of 49 M% and an e.e. of 94% were obtained after 70 h. 

Derivatives of cellulose continue to be used as supports for new affinity chromatography media and for immobilization of enzymes. Trypsin has been immobilized on 4-aminobenzyl-cellulose. A penicillinase from Bacillus cereus has been immobilized by glutaraldehyde-mediated reaction with aminoethyl-cellulose. Tannin has been activated with cyanogen bromide and coupled with... 

Unspecified reaction with agarose, dextran, aminoethyl-cellulose, and several inorganic carriers Active immobilized enzyme 741 o c ... 

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