Heinz body

A search for Heinz bodies Is helpful In the detection of an unstable hemoglobin, of a-thalassemia, homozygous 3-thalas-semla and related abnormalities, because hemoglobin often precipitates In the red cells of patients with one of these disorders. Inclusion bodies may consist of precipitated unstable hemoglobin, of 3 chalns (In a-thalassemia), or of a chains (In thalassemia) ... 

Figure 11. The formation of Heinz bodies in a subject with an unr stable hemoglobin (courte of Dr. E. Kleihauer, Vim, Germany)...

Red cells of a freshly collected blood sample will form Heinz bodies In the presence or absence of phenylhydrazlne In a greater number than red cells of a similar blood sample from a normal control. 

Case Condi- tion WBC Hb g% PCV % RBC lO /mm MCV MCH yyg MCHC % Heat d Stabil- ity Iso- pro- panol Free a Chains Heinz Bodies ... 

Heinz body preparations are positive as Is the heat stability test Electrophoretic examination of hemolysate showed the presence of a fast-moving variant which was readily separated from Hb-A by chromatography on DEAE-Sephadex The abnormal 3- chain was Isolated by CM-Cellulose chromatography as described before, and converted into the S-2-amlnoethyl (AE) derivative... 

May precipitate a Heinz body hemolytic anemia or methemoglobinemia... 

Mink 1-11 mo Hemato 45 M 262 M (increased Heinz body Bucci et al. 1997... 

Adverse effects (shortened RBC survival, increased Heinz body formation, increased number of reticulocytes, and reduced blood cell counts) were observed at 747 mg/kg/day in males and 907 mg/kg/day in females (average 827 mg/kg/day) (Bucci et al. 1994). Although not statistically significant, the number of Heinz bodies was increased relative to the controls and to the rats treated at 400 mg/kg/day. The observed effects are consistent with a direct effect on RBC and a decrease in their survival. 

This study is supported by intermediate-duration NOAELs for hematological effects of 75 mg/kg/day in a dog study (Hart 1980) and 45 mg/kg/day (males) and 57 mg/kg/day (females) in a mink study (Bucci et al. 1997). In the Bucci et al. (1997) mink study, the next highest level, 262 or 330 mg/kg/day in males and females, respectively, produced hematological changes that included increased Heinz body counts, reticulocytes, mean cell volume, and decreased RBC counts. 

In a study by MacEwen et al. (1970) using seven adult human volunteers, 10-min exposure to monomethylhydrazine (90 ppm, or 169 mg/m3) resulted in irritation of the eyes, nose, and throat but did not cause excessive lacrimation or coughing. Clinical chemistry parameters for 60 d following the exposure were not significantly affected a 3-5% increase in Heinz body formation at d 7 declined after 2 w. Additionally, spirometry tests revealed no exposure-related effects. 

Absence of Heinz bodies (Heinz bodies are more characteristic of G6PDH deficiency)... 

Answer E. Only option E is consistent with the constellation of clinical findings presented. Major clue is the positive Heinz body preparation. 

U.1S5 4h Heinz bodies in circulating red cells further exposure led to decrease in Heinz body formation Mouse 130. 131... 

Menzel, D. B., R. J. Slaughter. A, M. Bryant, and H. O. Jauregui. Heinz bodies formed in erythrocytes by fatty acid ozonides and ozone. Arch. Environ. Health 30 2%-301, 1975. 

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