Cellulose for chromatography

Cellulose for chromatography is purified by sequential washing with chloroform, ethanol, water, ethanol, chloroform and acetone. More extensive purification uses aqueous ammonia, water, hydrochloric acid, water, acetone and diethyl ether, followed by drying in a vacuum. Trace metals can be removed from filter paper by washing for several hours with O.IM oxalic or citric acid, followed by repeated washing with distilled water. 

Purification of cellulose for chromatography is by sequential washing with chloroform, ethanol, water, ethanol,... 

Cellulose for chromatography is a linear polymer of optically active D-glucopyranose units connected through l,4-/3 linkages in which the pyranose residues assume the energetically favored chair conformation its chains are arranged on a partially crystalline fiber structure with helical cavities. The... 

Purified from blood using CM-32 cellulose column chromatography. [Matsukawa et al. J Am Chem Soc 107 1108 1985.] For the purification of the a and P chains see Hill et al. Biochem Prep 10 55 1963. 

Thin layer chromatography was carried out on 20x20cm glass plates coated 0.25mm thick with a suitable support and dried overnight. Silica gel G, silica gel H and cellulose were examined as the solid phases for chromatography of methanearsonate, arsenite and arsenate. Several sprays for the visualization of the arsenicals on plates were tested. Three of the more successful reagents and the colour produced with final product are shown in Table 13.2. 

Paper chromatography and thin-layer chromatography (TLC) constitute the planar methods mentioned above. Paper chromatography makes use of a sheet of paper having the consistency of filter paper (cellulose) for the stationary phase. Since such paper is hydrophilic, the stationary phase is actually a thin film of water unintentionally adsorbed on the surface of the paper. Thus, paper chromatography represents a form of partition chromatography only. The mobile phase is always a liquid. 

Whatman CFll fibrous cellulose powder is prepared for chromatography... 

Use a sheet of polyethyleneimine cellulose (PEI Cellulose), 50 x 200 mm, for chromatography. Mark the start about 15 mm away of the shorter side with a pencil. Apply the sample with a pipet or syringe. If larger volumes have to be used, apply them in several portions and/or a short line. 

Hoffmann GF, Brendel SU, et al. (1992) Mevalonate kinase assay using DEAE-cellulose column chromatography for first-trimester prenatal diagnosis and complementation analysis in mevalonic aciduria. J Inherit Metab Dis 15 738-746... 

Solvent systems used for thin layer chromatography were 1) n-butanol acetic acidiwater (4 1 5 upper phase), 2) acetic acid water (15 85), 3) ethyl acetate pyridine water (12 5 4), and 4) chloroform acetic acid water (50 45 5). Silica gel plates were used for chromatography of flavonoid aglycones and cellulose plates for all other components. Aluminum chloride was used for detection (under long UV light) of flavonoids, aniline phthalate for sugars, ninhydrin for amino acids and iodine for other components. Cellulose thick layer plates were developed with solvents 1 or 2. 

Fractions H and /. Paper chromatography indicated that these two fractions were identical except for a few minor spots. The combined fractions were purified by cellulose column chromatography with the upper layer of 4 1 5 w-butanol-ethanol-water as the developer. Over 95% of the combined fraction was recovered as pure oxalic acid. 

A mixture of 0.44 g of acidic proteinoid, 0.19 g of basic proteinoid, and 0.31 g of radioactive ATP in 2.0 ml of 0.02 M MgCl2, 0.05 M Tris buffer, is heated in a boiling water bath for 5 min, and then incubated at 37 °C for 24 hrs, after the fractionation of the mixture by DEAE-cellulose column chromatography. Oligonucleotides produced are identified with those of authentic markers 47). Chain length has been determined by the ratio of AMP to adenosine in the alkaline hydrolyzate of the material which was treated by alkaline phosphatase to remove 5 - and 3 -phosphate47). 

The key to success in extensive purification and crystallization of ferredoxin is the use of diethylaminoethyl (DEAE) cellulose column chromatography. Ferredoxin has a marked affinity for DEAE-cellulose, and when a cell-free extract containing ferredoxin is passed through a DEAE-cellulose column, ferredoxin remains as a band at the top while contaminating protein passes through in the effluent. The affinity of ferredoxin for DEAE-cellulose may be explained by its isoelectric point of 3.7 (Lovenberg, Buchanan, and Rabinowitz (65)). 

PEI-thin layer plates Plastic sheets coated with PEI (polyethylene-imine)-cellulose for thin-layer chromatography. 20 x 20 cm sheets Polygram CEL 300 PEI/UV254 obtainable from Macherey-Nagel Co., 516 Diiren, Werkstrasse, 6-8, Postfach 307, West Germany or from laboratory suppliers. 

Chromatography of polysaccharides on silica gel and on carbon columns has been used to a limited extent. The use of ion-exchange resins has been investigated by Deuel and coworkers. The colunms had low capacities, and part of the material appeared to be irreversibly adsorbed. Deuel and coworkers, however, have reported better results when columns of cationic derivatives of cellulose, for instance (diethylaminoethyl)-cellulose, are used. Electrophoresis both by the Tiselius method on columns and on filter paper or glass-fiber sheets can give good separa-... 

A method for the separation of ellipticine (1), 9-methoxyellipticine (2), and 9-hydroxyellipticine (3) using cellulose adsorption chromatography (thin layer or paper) has been developed (27). The technique involves a solvent system consisting of a 50 50 mixture of 1.3 M ammonium sulfate and 96% acetic acid, followed by iodine vapor detection. 

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