Total internal reflection fluorescence application

N. L. Thompson and B. C. Lagerholm, Total internal reflection fluorescence Applications in cellular biophysics, Curr. Opin. Biotech. 8, 58-64 (1997). 

Burmeister JS, Olivier LA, Reichert WM, Truskey GA (1998) Application of total internal reflection fluorescence microscopy to study cell adhesion to biomaterials. Biomaterials 19 307-325... 

E. H. Hellen, R. M. Fulbright, and D. Axelrod, Total internal reflection fluorescence Theory and applications at biosurfaces, in Spectroscopic Membrane Probes (L. M. Loew, ed.), Vol. II, pp. 47-49, CRC Press, Boca Raton, Florida (1988). 

C. L. Poglitsch and N. L. Thompson, Substrate-supported planar membranes containing murine antibody Fc receptors A total internal reflection fluorescence microscopy study, in Biosensor Technology, Fundamentals and Applications (R. P. Buck, W. E. Hatfield, M. Umafiia, and E. F. Bowden, eds.), pp. 375-382, Marcel Dekker, New York (1990). 

D. Axelrod, R. M. Fulbright, and E. H. Hellen, Adsorption kinetics on biological membranes Measurement by total internal reflection fluorescence, in Applications of Fluorescence in the Biomedical Sciences (D. L. Taylor, A. S. Waggoner, F. Lanni, R. F. Murphy, and R. Birge, eds.), pp. 461-467, Alan R. Liss, New York (1986). 

The diffusion-related molecular processes occurring within a Cig stationary-phase have also been investigated using pyrene as a fluorescent probe [169]. Particular spectral bands were attributed to pyrene excimers formed in a diffusion-limited reaction. Rate constants for this formation were then used to estimate the microviscosity of the stationary-phase. A similar application of total internal reflection fluorescence... 

N. L. Thompson, K. H. Pearce, and H. V. Hsieh, Total internal reflection fluorescence microscopy Application to substrate-supported planar membranes, Eur. Biophys. J. 22, 367-378 (1993). 

Figure 2.6 Application of splinted RNA ligation procedure single molecule FRET. (A) Diagram of prism-type total internal reflection fluorescence microscope (TIRFM) for single molecule FRET measurements. (B) Distribution of single-molecule FRET values for dye-labeled telomerase RNA molecules generated by splinted RNA ligation. (C) Dye intensity and FRET traces of a single telomerase RNA molecule Cy3 emission (green), Cy5 emission (red), FRET ratio (blue).

Wellman AD, Sepaniak MJ (2007) Multiplexed, waveguide approach to magnetically assisted transport evanescent field fluoroassays. Anal Chem 79 6622-6628 Loete F, Vuillemin B, Oltra R et al (2006) Application of total internal reflection fluorescence microscopy for studying pH changes in an occluded electrochemical cell development of a waveguide sensor. Electrochem Commun 8 1016—1020... 

Total Internal Reflection Fluorescence Microscopy (TIRFM) is another useful tool for studying the reactions of individual molecules adsorbed, adhered or bound to surfaces. Typical applications are membrane fusion of vesicles [177], conformational and orientation changes [178] and lateral mobility of molecules [179],... 

On the other hand, optical microscopy, confocal microscopy, ellipsometry, scanning electron microscopy (SEM), scanning tunneling microscopy (STM), atomic force microscopy (AFM) and total internal reflection fluorescence (TIRF) are the main microscopic methods for imaging the surface structure. There are many good books and reviews on spectroscopic and chemical surface analysis methods and microscopy of surfaces description of the principles and application details of these advanced instrumental methods is beyond the scope of this book. 

The application of total internal reflection fluorescence spectroscopy (TIRF) by this laboratory to the study of protein adsorption at solid-liquid interfaces is reviewed. TIRF has been used to determine adsorption isotherms and adsorption rates from single-and multi-component protein solutions. Initial adsorption rates of BSA can be explained qualitatively by the properties of the adsorbing surface. Most recently, a TIRF study using monoclonal antibodies to probe the conformation of adsorbed sperm whale myoglobin (Mb) elucidated two aspects of the Mb adsorption process 1) Mb adsorbs in a non-random manner. 2) Conformational changes of adsorbed Mb, if they occur, are minor and confined to local regions of the molecule. Fluorescence energy transfer and proteolytic enzyme techniques, when coupled with TIRF, can characterize, respectively, the conformation and orientation of adsorbed Mb. 

Application of total internal reflection fluorescence Dependent on tbe combined method 171... 

In micro- and nanoscale fluid mechanics, measurements of mass transport and fluid velocity are used to probe fundamental physical phenomena and evaluate the performance of microfluidic devices. Evanescent wave illumination has been combined with several other diagnostic techniques to make such measurements within a few hundred nanometers of fluid—solid interfaces with a resolution as small as several nanometers. Laser Doppler velocimetry has been applied to measure single-point tracer particle velocities in the boundary layer of a fluid within 1 pm of a wall. By seeding fluid with fluorescent dye, total internal reflection fluorescence recovery after photobleaching (FRAP) has been used to measure near-wall diffusion coefficients and velocity (for a summary of early applications, see Zettner and Yoda [2]). 

Total internal reflection fluorescence microscopy (TIRFM), or simply TIRF, is the application of fluorescence imaging to evanescent-wave microscopy. The material of interest is tagged with fluorescent material that emits light at wavelengths greater than the excitation photons from the evanescent field, which additionally improves the detectability of the evanescent-wave microscopy technique. 

K. Stock, R. Sailer, W.S.L. Strauss, M. Lyttek, R. Steiner, H. Schneckenburger Variable-angle total internal reflection fluorescence microscopy (VA-TIRFM) realization and application of a conqjact illumination device, J. Microsc. 211 (2003), 19-29. 

Abstract Recent development in total internal reflection fluorescence microscopy (TIRFM) has made it possible to directly monitor the behaviors of biomolecules at tbe single-molecule level both in vitro and in living cells. This technique has opened a new era in bioscience, so-called single-molecule nanobioscience. In this review, we will summarize the theoretical basis of TIRFM, explain the instruments of TIRFM for single-molecules imaging, and discuss some novel applications of TIRFM to single-molecule nanobioscience. 

Axelrod D, HeUen EH, Fulbright RM (1992) Total internal reflection fluorescence. In Lakowicz JR (ed) Topics in fluorescence spectroscopy. Vol 3. Biochemical applications. Plenum Press, New York, p 289... 

The upgrade of a frequency-domain fluorescence lifetime imaging microscope (FLIM) to a prismless objective-based total internal reflection-FLIM (TIR-FLIM) system is described. By off-axis coupling of the intensity-modulated laser from a fiber and using a high numerical aperture oil objective, TIR-FLIM can be readily achieved. The usefulness of the technique is demonstrated by a fluorescence resonance energy transfer study of Annexin A4 relocation and two-dimensional crystal formation near the plasma membrane of cultured mammalian cells. Possible future applications and comparison to other techniques are discussed. 

Aslan K, Holley P, Geddes CD (2006) Microwave-accelerated metal-enhanced fluorescence (MAMEF) with silver colloids in 96-well plates Application to ultra fast and sensitive immunoassays, high throughput screening and dmg discovery. J Immunol Methods 312 137-147 Matveeva E, Gryczynski Z, Malicka J et al (2004) Metal-enhanced fluorescence immunoassays using total internal reflection and silver-coated surfaces. Anal Biochem 334 303-311 Blue R, Kent N, Polerecky L (2005) Platform for enhanced detection efficiency in luminescent-based sensors. Electron Lett 41 682-684... 

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