Total Internal Reflection Fluorescence Microscopy

Tokunaga M, Kitamura K, Saito K, Iwane A H and Yanagida T 1997 Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy Biochem. Biophys. Res. Commun. 235 47-53... 

Axelrod, D. (2001). Total internal reflection fluorescence microscopy in cell biology. Traffic 2, 764-74. 

Beaumont, V. (2003). Visualizing membrane trafficking using total internal reflection fluorescence microscopy. Biochem. Soc. Trans. 31, 819-23. 

Khakh, B. S., Fisher, J. A., Nashmi, R., Bowser, D. N. and Lester, H. A. (2005). An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy. J. Neurosci. 25, 6911-20. 

Burmeister JS, Olivier LA, Reichert WM, Truskey GA (1998) Application of total internal reflection fluorescence microscopy to study cell adhesion to biomaterials. Biomaterials 19 307-325... 

Shino A, Lujioka K, Manabe N, Yamaya S, Goto Y, Yasuhara M, Yamamoto K (2005). Simultaneous multicolor detection system of the single-molecular microbial antigen with total internal reflection fluorescence microscopy. Microbiol. Immunol. 49 461 —170. 

D. Axelrod, Total internal reflection fluorescence microscopy, in Fluorescence Microscopy of Living Cells in Culture B (D. L. Taylor and Y.-L. Wang, eds.), Methods in Cell Biology, Vol. 30, pp. 245-270, Academic Press, San Diego, California (1989). 

C. L. Poglitsch and N. L. Thompson, Interaction of antibodies with Fc receptors in substrate-supported planar membranes measured by total internal reflection fluorescence microscopy, Biochemistry 29, 248-254 (1990). 

C. L. Poglitsch and N. L. Thompson, Substrate-supported planar membranes containing murine antibody Fc receptors A total internal reflection fluorescence microscopy study, in Biosensor Technology, Fundamentals and Applications (R. P. Buck, W. E. Hatfield, M. Umafiia, and E. F. Bowden, eds.), pp. 375-382, Marcel Dekker, New York (1990). 

A. Kurahashi, A. Itaya, H. Masuhara, M. Sato, T. Yamada, and C. Koto, Depth distribution of fluorescent species in silk fabrics as revealed by total internal reflection fluorescence microscopy, Chem. Lett. 1986, 1413-1416. 

F. Lanni, A. S. Waggoner, and D. L. Taylor, Structural organization of interphase 3T3 fibroblasts studied by total internal reflection fluorescence microscopy, J. Cell Biol. 100, 1091-1102 (1985). 

To discriminate between surface bound protein molecules and those in bulk solution, total internal reflection fluorescence microscopy (TIRFM)41 55 was employed. TIRFM creates an evanescence wave that decays as a function of distance from the surface as ... 

N. L. Thompson, K. H. Pearce, and H. V. Hsieh, Total internal reflection fluorescence microscopy Application to substrate-supported planar membranes, Eur. Biophys. J. 22, 367-378 (1993). 

N. L. Thompson, A. W. Drake, L. Chen, and W. V. Broek, Equilibrium, kinetics, diffusion and selfassociation of proteins at membrane surfaces Measurement by total internal reflection fluorescence microscopy, Photochem. Photobiol. 65, 39-46 (1997). 

TIRFM total internal reflection fluorescence microscopy TLM thermal lens microscopy... 

Complementary microscopy techniques can be used to follow the morphology and growth of fibrils either on a surface or in aliquots taken from the assembly solution including total internal reflection fluorescence microscopy (TIRFM) (Ban et al., 2004), transmission electron microscopy (TEM), or atomic force microscopy (AFM). 

Fig. 12. The figure at the left is the schematic illustration of laser induced total internal reflection fluorescence microscopy for the single molecule detection at the liquid-liquid interface. Abbreviations ND ND filter, 1/2 1/2 plate, M mirror, L lens, C microcell, O objective (60 x), F band path filter, P pinhole, APD avalanche photodiode detector. The figure at the right shows the composition of the microcell.

A. Yu. Kobitski, C. D. Heyes, and G. U. Nienhaus, Total internal reflection fluorescence microscopy—a powerful tool to study single quantum dots. Appl. Surface Sci. 234 86-92 (2004). 

Real-Time Observation of Amyloid Fibril Growth by Total Internal Reflection Fluorescence Microscopy... 

Fig. 15.1. Schematic representation of amyloid fibrils revealed by total internal reflection fluorescence microscopy, (a) The penetration depth of the evanescent field formed by the total internal reflection of laser light is 150nm for a laser light at 455 nm, so only amyloid fibrils lying parallel to the slide glass surface were observed. (b) Schematic diagram of a prism-type TIRFM system on an inverted microscope. ISIT image-intensifier-coupled silicone intensified target camera, CCD charge-coupled device camera...

SPR imaging was also employed to look at the same cholera toxin-GMl interaction on a supported bilayer by Philips et al. in array experiments [74]. Another investigative technique applied to the model ganglioside-cholera toxin system was total internal reflection fluorescence microscopy applied to spatially addressable supported bilayer membranes [75]. The recognition by HIV-1 surface glycoprotein gpl20 of glycosphingolipids partitioned within an SBM has also been looked at by total internal reflection fluorescence [76]. 

FIGURE 10.7. Total internal reflection fluorescence microscopy of the micro-two-phase system of dode-cane/water. (a) Continuous photons with an average of 11 molecules in the observation area, (b) photon burst with an average of 0.02 molecules in the observation area and (c) extension of the photon burst upon the addition of DMPC. A model for the photon burst upon the observation using single molecule diffusion (d). 

Wellman AD, Sepaniak MJ (2007) Multiplexed, waveguide approach to magnetically assisted transport evanescent field fluoroassays. Anal Chem 79 6622-6628 Loete F, Vuillemin B, Oltra R et al (2006) Application of total internal reflection fluorescence microscopy for studying pH changes in an occluded electrochemical cell development of a waveguide sensor. Electrochem Commun 8 1016—1020... 

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