Microwave-accelerated metal-enhanced fluorescence

Aslan K, Holley P, Geddes CD (2006) Microwave-accelerated metal-enhanced fluorescence (MAMEF) with silver colloids in 96-well plates Application to ultra fast and sensitive immunoassays, high throughput screening and dmg discovery. J Immunol Methods 312 137-147 Matveeva E, Gryczynski Z, Malicka J et al (2004) Metal-enhanced fluorescence immunoassays using total internal reflection and silver-coated surfaces. Anal Biochem 334 303-311 Blue R, Kent N, Polerecky L (2005) Platform for enhanced detection efficiency in luminescent-based sensors. Electron Lett 41 682-684... 

In the past few years our research laboratory has been working on the applications of a new technique, called microwave-accelerated metal-enhanced fluorescence (MAMEF), as applied to fluorescence-based bioassays. The MAMEF technique couples the benefits of low power microwave heating witii metal-enhanced fluorescence (MEF), to address the two major shortcomings of fluorescence-based bioassays currently in use today i.e., bioassay sensitivity and rapidity. In MAMEF, [1] the MEF phenomenon increases the sensitivity of the assays, while the use of low power microwave heating kinetically accelerates assays to completion within only a few seconds. 

Microwave-Accelerated Metal-Enhanced Fluorescence (MAMEF)... 

In this review chapter, the summary of recent work on a unique technique, called microwave-accelerated metal-enhanced fluorescence (MAMEF), which affords for ultra fast and sensitive fluorescence-based detection of biological materials of interest, is presented. MAMEF is a new bioassay platform technique that couples the benefits of Metal-Enhanced Fluorescence (MEF) with low power... 

Aslan, K. and Geddes, C. D. (2005). Microwave-accelerated metal-enhanced fluorescence Platform technology for ultrafast and ultrabright assays Analytical chemistry 77 8057-8067. 

Dragan, A. I., Golberg, K., Elbaz, A., Marks, R., Zhang, Y., Geddes, C. D. (2011). Two-color, 30 second microwave-accelerated Metal-Enhanced Fluorescence DNA assays A new Rapid Catch and Signal (RCS) technology. /. Immunohg. Meth, 366, pp. 1-7, ISSN 0022-1759... 

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