Membrane probe

Confirmation that FMLP-induced activation involves release of intracellular calcium was obtained by loading neutrophils with CTC. Addition of 20 pH CTC to a neutrophil suspension resulted in a gradual increase in CTC fluorescence as the probe entered the cells and partitioned into intracellular membranes (Figure 9, upper panel). Addition of FMLP resulted in an abrupt decrease in fluorescence, suggesting release of calcium from intracellular membranes probed by CTC. The FMLP-induced release of calcium monitored by CTC was little affected by increased medium osmolality a similar fluorescence decrease was seen in the presence of sodium HEPES (645 mosmol/kg) or sodium sulfate (662 mosmol/kg) (Figure 9, lower panel). 

Kusumi, A., W. K. Subczynski, and J. S. Hyde. 1982a. Effects of pH on ESR spectra of stearic acid spin labels in membranes Probing the membrane surface. Fed. Proc. 41 1394. 

Chattopadhyay A, Mukherjee S (1999) Red edge excitation shift of a deeply embedded membrane probe implications in water penetration in the bilayer. J Phys Chem B 103(38) 8180-8185... 

Loew LM (1988) How to choose a potentiometric membrane probe. In Loew LM (ed) Spectroscopic membrane probes, vol 2. CRC Press, Boca Raton, FL... 

Amoroso S, Agon VV, Starke-Peterkovic T et al (2006) Photochemical behaviour and Na+, K+-ATPase sensitivity of voltage-sensitive styrylpyridinium fluorescent membrane probes. Photochem Photobiol 82 495-502... 

Sklar, L. A., Hudson, B. S. and Simoni, R. D. (1976a). Conjugated linear polyene fatty-acids as membrane probes. Fed. Proc. 35,1531-1531. 

Other methods for the determination of chlorine in seawater or saline waters are based on the use of barbituric acid [13] and on the use of residual chlorine electrodes [ 14] or amperometric membrane probes [15,16]. In the barbituric acid method [12], chlorine reacts rapidly in the presence of bromide and has completely disappeared after 1 minute. This result, which was verified in the range pH 7.5-9.4, proves the absence of free chlorine in seawater. A study of the colorimetric deterioration of free halogens by the diethylparaphenylene-diamine technique shows that the titration curve of the compound obtained is more like the bromine curve than that of chlorine. The author suggests... 

Fig. 17. A photoaffinity probe for integrin signaling and photoactivatable membrane probes...

Photoaffinity Labeling of Steroid Hormone Receptors and Membrane Probes... 

To date, there have been only very few studies on the sorption of environmentally relevant HIOCs to membranes. In contrast, there exists a large body of literature on ionogenic drug partitioning (see, e.g. [3-5]), and the partitioning of hydrophobic ions [6], and the use of fluorescent membrane probes [7], This literature review attempts to relate the findings from these related scientific fields to environmental chemistry. 

E. Heinzle, H. Kramer, and I. J. Dunn, State analysis of fermentation using amass spectrometer with membrane probe, Biotechnol. Bioeng. 27, 238-246(1985). 

B. Hudson and S. A. Cavalier, in Studies ofMembrane Dynamics and Lipid-Protein Interactions with Parinaric Acid in Speclroscopic Membrane Probes Vol. 1 (L. Loew, ed.), CRC Press, Inc., Boca Raton, Florida (1988). 

M. Cranney, R. B. Cundall, G. R. Jones, J. T. Richards, and E. W. Thomas, Fluorescence lifetime and quenching studies on some interesting diphenythexatriene membrane probes,... 

R. A. Parente and B. R. Lentz, Advantages and limitations of l-palmitoyl-l-[[2-[4-(6-phcn l-//Y ( .v-l,3,5-hcxatrienyl (phenyl ]ethyl ]carbonyl ]-3-sn-phosphatidylcholine as a fluorescent membrane probe, Biochemistry 24, 6178-6185 (1985). 

A. M. Kleinfeld and M. F. Lukacovic, Energy-transfer study of cytochrome b5 using the anthroyloxy fatty acid membrane probes, Biochemistry 24, 1883-1890 (1985). 

D. B. Chalpin and A. M. Kleinfeld, Interaction of fluorescence quenchers with the -(9-anthroyloxy) fatty acid membrane probes, Biochim. Biophys. Acta 731, 465 174 (1983). 

Janie EM, Kissinger PT. Monitoring physiological variables with membrane probes. Acta Astronautica 1998, 43, 87-99. 

Mendes, M.A. and M.N. Eberlin. 2000. Trace level analysis of VOCs and semi-VOCs in aqueous solution using a direct insertion membrane probe and trap and release membrane introduction mass spectrometry. Analyst 125 21-24. 

Direct potentiometric determination by using an ammonia-sensing membrane probe... 

T. Parasassi, E.K. Krasnowska, L. Bagatolli, E. Gratton, Laurdan and prodan as polarity-sensitive fluorescent membrane probes. J. Fluor. 8,365 (1998)... 

Once the DNA has been purified, it is used unsheared when attached to membranes for free solution reactions and for membrane probes, however, it must be sheared to satisfy reaction kinetics and to maintain specificity. Strand lengths of 400-500 base pairs (bp) are usually employed.2 8 The French pressure cell is commonly used for shearing, and a double... 

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