Thin-layer chromatography pharmaceutical applications

Duez, P., Chamart, S., and Hanocq, M. (1991). Postchromatographic derivatization in quantitative thin layer chromatography Pharmaceutical applications. J. Planar Chromatogr.—Mod. TLC 4 69—76. 

The technique of thin-layer chromatography (TLC) has been used extensively in the domain of pharmaceutical analysis for a variety of specific and useful applications, for example ... 

Taylor and co-workers further demonstrated the value of open-access LC/MS systems for generating a widened scope of pharmaceutical analysis applications, including (1) characterization of synthetic intermediates and target compounds (2) reaction monitoring (3) reaction optimization (4) analysis of preparative HPLC fractions and (5) analysis of thin layer chromatography (TLC) plate spots. The availability of these methods led to the increased use of LC/MS for structural analysis. The short analysis time and reliable structure confirmation resulted in the use of LC/MS as a first choice for structure characterization for synthetic chemistry applications, as well as an expanded, and perhaps, integrated role of sample generator and analyst. 

Thin layer chromatography is still used in pharmaceutical research and development as a very simple and cost effective technique. A chapter is devoted to summaries the basic principles of thin layer chromatography and the pharmaceutical applications of the technique. 

Basis and pharmaceutical applications of thin layer chromatography... 

Basis and pharmaceutical applications of thin-layer chromatography TABLE 10.7 BINDERS USED FOR STATIONARY PHASE PLANAR CHROMATOGRAPHY 455... 

The history, theory, and applications of paper and thin-layer techniques are described in several books. " The first report on the subject of thin-layer chromatography was by Izmailov and Shraiber, who applied the technique to the separation of pharmaceutical tinctures. 

Because of the biological and pharmaceutical interest in phenothiazine derivatives, these compounds have been used in a variety of analytical studies and applications. For a number of years, several analytical methods have been proposed by various groups for the determination of phenothiazine and its derivatives in pharmaceutical formulations and biological fluids, including thin-layer chromatography (TLC) [51-54], high-performance liquid chromatography (HPLC) [55-58], spectrofluorimetry, either direct or with derivatization [59-64], flow-injection analysis (FIA) [10,65-73] and other techniques [11,74-76]. 

See also Clinical Analysis Sample Handling. Derivatization of Analytes. Forensic Sciences Systematic Drug Identification Thin-Layer Chromatography. Fourier Transform Techniques. Gas Chromatography Mass Spectrometry Forensic Applications. Immunoassays, Applications Clinical Forensic. Liquid Chromatography Normal Phase Reversed Phase. Spectrophotometry Pharmaceutical Applications. 

See also Atomic Absorption Spectrometry Principles and Instrumentation. Atomic Emission Spectrometry Inductively Coupled Plasma. Flow Injection Analysis Principles Instrumentation Detection Techniques Environmental and Agricultural Applications Clinical and Pharmaceutical Applications Industrial Applications. Microelectrodes. Sensors Chemically Modified Electrodes. Thin-Layer Chromatography Overview. Water Analysis Freshwater Seawater - Inorganic Compounds. 

Christie (1990), in an overview of TLC, asks the question Has thin-layer chromatography had its day . Comparison of this simple technique with the newer and more sophisticated high pressure liquid chromatography (HPLC) makes TLC look obsolete. However, as also agreed by the author, traditional TLC is still used and is likely to be around for many years to come. Between the years 1990 and 1996 TLC has found application in several fields, and publications relate to food (18%), medical, clinical and biological applications (26%), pharmaceuticals (25%), environmental studies (11%), detection (14%) and chemical synthesis monitoring (6%) (Weins and Hauck, 1995). It is estimated that there were between 500-900 articles per... 

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