TLG, thin layer gel

TLG. Thin-layer gel chromatography, in which separations are based mainly on solute sizes. 

Thin layer gel chromatography. Determann, and Johanson and I nno (in 1962) showed that gel permeation chromatography could be conducted using thin layers of gel. For clinical use, thin layer gel filtration (TLG) is ideal since very small sample volinne is required for this technique. In this technique, a layer of hydrated gel is applied to the plate. There is no need of a fixative to adhere the gel beads. The plate is not dried at all and is placed in an airtight container at an angle of 20 . The plate is connected to reservoirs at either end by means of filter paper bridges. Equilibrium must be carried out for at least 12 hours. Such equilibrium serves to normalize the ratio between the stationary and mobile phase volumes. The sample may be applied either as a spot or as a band. TTie plate may then be developed for a suitable time and the sep2u-ated components detected by suitable means. 

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