Sodium water sterilization

A spore suspension was prepared with 5 ml of a 0.9% sodium chloride solution obtained from a 7-10 day old, malt extract-yeast extract agar slant culture of Mortierella maculata nov. spec. E-97 [NCAIM(P)F 001266] strain able to 6-p-hydroxylate compactin and the suspension was used to inoculate 100 ml inoculum medium PI (glucose-50 g, soybean meal-20 g, in 1000 ml tap water) sterilized in a 500 ml Erlenmeyer flask. 

Preparation of sodium acetate buffer. To prepare sodium acetate (NaAc) buffer (pH 5.2), dilute 4.2 mL of 3 M sodium acetate into 495.8 mL of deionized water. Sterilize by vacuum filtration through a 0.2 im filter. 

Thiotepa. USP. Thiotepa, TSPA. NSC-6396, N,N, N"-triethylene-thiophasphoramide, tris(l-aziridinyl)phosphine suindc, is pteixired by treating trichlorophosphinc sulfide with aziridine and is obtained as a white powder that is water soluble. It is supplied in vials containing IS mg of thiotepa. 80 mg of sodium chloride, and SO mg of sodium bicarlMnate. Sterile water is added to make an isotonic solution. Both the vials and solutions must be stored at 2 to 8°C. These solutions may be stored S days without loss of potency. 

Running buffer (5 X ) 20.9 g 3-(N-morpholino)propane sulfonic acid (MOPS, free acid from Sigma) in 800 ml of water containing 50 mM sodium acetate adjust pH to 7.0 by adding NaOH (or acetic acid). Add 10 ml of DEPC-treated 0.5 M EDTA and adjust the volume to I I with water. Sterilize by filtration through a 0.2 pm Millipore filter and store in the dark (when buffer becomes strongly colored, it should be discarded). See Table 9.11 for an alternative to MOPS buffer. 

Deionized sterile water Sterilize double-distilled water by treatment with DEP as a 1% solution followed by autoclaving at 120°C for 20 min. When cool, add sodium azide (0.001%) to inhibit microbial growth. Water sterilized in this way can be used safely for a 2-wk period. Whenever in doubt, discard water and sterilize a new batch. 

Where free chlorine is present, eg, in drinking water, it is measured on-site, and a crystal (eg, 10 mg/40 mL) of sodium thiosulfate is added to the botde prior to sterilization to convert free chlorine to chloride. 

The diffusion process has not been designed to ensure sterility, although temperatures above 65°C significantly retard microbial activity. Sulfur dioxide, thiocarbamates, glutaraldehyde, sodium bisulfite, and chlorine dioxide are all used, occasionally disregarding their redox incompatibilities, to knock down or control infections. The most common addition point is to the water from the pulp presses as it is returned to the diffuser. Surfactants ate almost... 

Fluoro-l 13,17ot-Dihydroxy-21-Acetoxy-1,4-Pregnadiene-3,20-Dione A medium consisting of 1% dextrose hydrate, 2% cornsteep liquor of 60% solids and Kalamazoo tap water was adjusted to pH 4.9 with sodium hydroxide. The medium was steam sterilized at 15 pounds pressure for 30 minutes, cooled, and then inoculated with a 24-hour growth, from spores, of Septomyxa affinis, ATCC 6737. The medium was agitated, sparged with sterile air at the rate of one-tenth volume of air per volume of medium per minute. At the end of 24 hours of fermentation at room temperature, the pH was about 7.4. 

Then, 1.3 ml of glycerine are mixed with 0.5 ml of a 25% solution of methyl p-hydroxy-benzoate in ethanol, and 50 ml of distilled water are added. To the produced mixture are, after sterile filtration, added 10 ml of the stock solution 1, 2.5 ml of the stock solution 2 and 10 ml of the stock solution 3, after which 3.0 ml of sterile 0.1 N sodium hydroxide are added, and the mixture is filled up with sterile distilled water to a volume of 100 ml. The insulin will be precipitated amorphously by the admixture of the sodium hydroxide, and the produced suspension acquires the pH value of 7. It will contain approximately 1 gamma zinc per insulin unit. 

As described in U.S. Patent 2,929,763, methandrostenolone may be made by a fermentation route. 2 g of sodium nitrate, 1 g of primary potassium orthophosphate, 0.5 g of magnesium sulfate heptahydrate, 0.5 g of potassium chloride, 50 g of glucose and 1 g of Difco yeast extract are dissolved in one liter of tap water, brought to pH 5 by addition of a sodium hydroxide solution and sterilized. The resulting nutrient solution is inoculated with 50 cc of a 4-day-old shaking culture of Didyniel/a lycopersici and shaken for 48 hours at 27 C, whereby the culture becomes well developed. 

Preparation of Sodium 1-Methyl-5-Allyl-5-(1-Methyl-2-Pentynyl) Barbiturate A solution of 61 g of 1-methyl-5-allyl-5-(1-methyl-2-pentynyl) barbituric acid in 100 ml of ether was extracted with 465 ml of 2% aqueous sodium hydroxide solution. The aqueous extract was washed with successive 75 ml and 50 ml portions of ether. The pH of the aqueous solution was adjusted to 11.7, using 5% aqueous sodium hydroxide solution. 5 g of decolorizing carbon were added to the solution with stirring the mixture was permitted to stand for 20 minutes at room temperature, and the carbon was removed by filtration. A solution containing 4 g of sodium carbonate in 25 ml of water was added to the aqueous solution, and the mixture was filtered sterile through a porcelain filter candle of 02 porosity into sterile bottles. The aqueous solution was then dried from the frozen state, whereupon a sterile residue of sodium 1-methyl-5-allyl-5-(1-methyl-2-pentynyl) barbiturate, weighing about 62 g was obtained. 

Sterile agar slants are prepared using the Streptomyces sporulation medium of Hickey and Tresner, J. Bact., vol. 64, pages 891-892 (1952). Four of these slants are inoculated with lyophilized spores of Streptomyces antibioticus NRRL 3238, incubated at 28°C for 7 days or until aerial spore growth is well-advanced, and then stored at 5°C. The spores from the four slants are suspended in 40 ml of 0.1% sterile sodium heptadecyl sulfate solution. A nutrient medium having the following composition is then prepared 2.0% glucose monohydrate 1.0% soybean meal, solvent extracted, 44% protein 0.5% animal peptone (Wilson s protopeptone 159) 0.2% ammonium chloride 0.5% sodium chloride 0.25% calcium carbonate and water to make 100%. 

Methicillin sodium To reconstitute 1 g vial add 1.5 mL of sterile water for injection or sodium chloride injection. Each reconstituted mL contains approximately 500 mg of methicillin. 

Mechlorethamine Cold packs Sodium thiosulfate Prepare 1/6 M solution by adding 4 mL of 10% solution to 6 mL sterile water inject 2 mL for each mg of mechlorethamine. Follow with 1 mL SQ (0.1 mL doses clockwise around area) may repeat every 3-4 hours if needed. Sodium thiosulfate must be diluted prior to administration. 

The pungent and irritating odor of chloramines is often mistaken for the chlorine odor of swimming pools. Chloramines form from the combination of sodium hypochlorite (added to sterilize the water) and nitrogen-containing compounds that are human waste by-products. 

Barbula convoluta, Tortella flavovirens and Pleurochaete squarrosa, spp. (that only sporadically produce sporophytes), were collected and cleaned from dust and dead parts, thoroughly washed in tap water and then in solution of Triton X-100 (0.8%), rinsed in distilled water and surface sterilized for 10-20 seconds in 1% sodium hypochlorite solution and rinsed four times in sterilized distilled water. 

Fragments of C. foliacea thalli were stored dry. Before use, these were abundantly rinsed in tap water and rapidly surface sterilized (10 seconds) in 1% sodium hypochloride. They were then inoculated in the in ratio of 0.5 g Cladonia thallus to each Petri dish containing 20 ml medium (water or Mohr) with 200 pi spore suspension (see above) or 20 shoot fragments. 

---