Schneider-2 cells

The polymer must have a high molar mass. PEG at a range of 6-20 kDa is commonly used (Ersson et al., 1998). Precipitation with PEG was used, for instance, by Demi et al. (1999) as one of the purification steps for the isolation of hepatitis B virus surface antigen (HBsAg) from the insect cell culture supernatant of genetically modified DS-2 Drosophila melanoga-ster Schneider-2) cells. 

Demi L, Schirmbeck R, Reimann J, Wolf H, Wagner R (1999), Purification and characterization of hepatitis B virus surface antigen particles produced in Drosophila Schneider-2 cells, J. Virol. Methods 79 205-217. 

This cell line is derived from the late embryonic state of Drosophila melanogaster and is used to produce recombinant proteins, e.g. human a -antitrypsin. Schneider-2 cells are adaptable to large-scale cultivation in stirred tank bioreactors and show many of the properties of Sf9 cells. 

Transcript 1 mRNAs represent 11 percent of the desat transcripts. They are principally found in Schneider cell culture (Schneider cells originated from embryonic cells which became immortalized) but are less represented in embryos and larvae (10 to 12 percent of the desatl transcripts). In the adult they constitute only 3 percent of the desat transcripts in head but seem more abundant in testis (two out of two) and ovary (two out of five). In the proximal 5 -non-transcribed region are found two TATA and two CAAT boxes. 

Some types of cultured cells, such as Drosophila Schneider cells, can be grown directly on glass microscope cover slips. Such cells can be subjected to FISH procedures as easily as they can be immunostained. Here is a simple procedure for fixation ... 

Quednau J and Schneider G M 1989 A new high-pressure cell for differential pressure-jump experiments using optical detection Rev. Sc/. Instnim. 60 3685-7... 

Schneider, U. Mazur, P. (1987). Relative influence of unfrozen fiwition and salt concentration on the survival of slowly frozen eight-cell mouse embryos. Cryobiol. 24, 17-41. 

Fargnoli, J., Kunisada, T., Fomace, A.J.. Schneider, E.L., Holbrook, N.J. (1990). Decreased expression of heat shock protein 70 mRNA and protein after heat treatment in cells of aged rats. Proc. Natl. Acad. Sci. USA 87, 846-850. 

Mack M, Schneider MA. Moll C. Cihak J. Bruhl H. Ellwart JW Hogarth MP. Stangassinger M, Sch-londorff D Identification of antigen-capturing cells as basophils. J Immunol 2005 174 735-741. 

Ellis, W. G. Schneider E. L. McCulloch, J. R. Suzuki R. and Epstein, C. J. "Fetal Globoid Cell Leukodystrophy (Rrabbe s Disease) Pathological and Biochemical Examination". Arch. Neurol., (1973), 29, 253-257. 

Schaefer U, Schneider A, Rixen D, Neugebauer E (1998) Neutrophil adhesion to histamine stimulated cultured endothelial cells is primarily mediated via activation of phospholipase C and nitric oxide synthase isozymes. Inflamm Res 47(6) 256-264 Schaefer U, Schmitz V, Schneider A, Neugebauer E (1999) Histamine induced homologous and heterologous regulation of histamine receptor subtype mRNA expression in cultured endothelial ceUs. Shock 12(4) 309-315... 

Kasten, F. H., Pineda, L. F., Schneider, P. E., Rawls, H. R. Foster, T. A. (1989). Biocompatibility testing of an experimental fluoride releasing resin using human gingival epithelial cells in vitro. In Vitro Cellular Development Biology, 25, 57-62. 

Siems, W.G., Grune, T., Werner, A., Gerb er, G., Buntrock, P. and Schneider, W. (1992). Protective influence of oxypurinol on the trinitrobenzene sulphonic acid (TNB) model of inflammatory bowel disease in rats. Cell Mol. Biol. 38, 189-199. 

Bruhl H, Cihak J, Schneider MA, et al. Dual role of CCR2 during initiation and progression of collagen-induced arthritis evidence for regulatory activity of CCR2+ T cells. J Immunol 2004 172(2) 890-898. 

Schneider, J.C., Jenings, A.F., Mun, D.M. et al. (2005) Auxotrophic markers pyrF and proC can replace antibiotic markers on protein production plasmids in high-cell-density Pseudomonas fluorescens fermentation. Biotechnology Progress, 21 (2), 343-348. 

Gu, W., Schneider, J.W., Condorelli, G., Kaushal, S., Mahdavi, V. and Nadal-Ginard, B. (1993) Interaction of myogenic factors and the retinoblastoma protein mediates muscle cell commitment and differentiation. Cell 72, 309-324. 

Singh, N.P., McCoy, M.T., Tice, R.R. and Schneider, E.L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research 175 184-191. 

Rzadzinska, A. K., Schneider, M. E., Davies, C., Riordan, G. P. and Kachar, B. An actin molecular treadmill and myosins maintain stereocilia functional architecture and selfrenewal. J. Cell Biol. 164 887-897, 2004. 

Schneider TW, SchesslerHM, Shaffer KM, Dumm JM, Younce LA (2001) Surface patterning and adhesion of neuroblastoma X glioma (NG108-15) cells. Biomed Microdev 3 4 315... 

Exposure of cells to fluorescent light during the culture period leads to photolysis of BrdU-containing DNA and a concomitant increase in SCE frequency (Wolff and Perry, 1974). Consequently, SCE cultures should be kept in the dark and manipulated under subdued light conditions such as yellow safe light. Furthermore, media used in SCE assays should be stored in the dark, since certain media components produce reactive SCE-inducing intermediates on exposure to fluorescent light (Monticone and Schneider, 1979). 

Monticone, R.E. and Schneider, E.L. (1979). Induction of SCEs in human cells by fluorescent light. Mutation Res. 59 215-221. 

Ferin, J. (1977). Effect of particle content of lung on clearance pathways. In Pulmonary Macrophage and Epithelial Cells (Sanders, C.L., Schneider, R.P., Dagle, G.E. and Ragan, H.A., Eds.). Technical Information Center, Energy Research and Development Administration, Springfield, VA, pp. 414-423. 

Stebbins, C. E., Russo, A. A., Schneider, C., Rosen, N., Hard, F. U., and Pavledch, N. P. (1997). Crystal structure of an Hsp90-geldanamycin complex targeting of a protein chaperone by an antitumor agent. Cell 89, 239—250. 

Pearce AI, Richards RG, Milz S, Schneider E, Pearce SG (2007) Animal models for implant biomaterial research in bone a review. Eur Cells Mater 13 1-10. 

Schneider, K.C., C. Bradbeer, R.N. Singh, L.C. Wang, P.W. Wilson and R.H. Burris. Nitrogen fixation by cell-free preparations from microorganisms. Proc. Natl. Acad, Sci. USA 46, 726-733 (1960). 

Schneider AI, Maier-Reif K, Graeve T. Constructing an in vitro cornea from cultures of the three specific comeal cell types. In Vitro Cell Dev Biol Anim 35 515-526 (1999). 

Schneider, M.H. (1995). New cell wall and cell lumen wood polymer composites. Wood Science and Technology, 29(4), 121-127. 

Schneider, M.H., Brebner, K.I. and Hartley, I.D. (1991). Swelling of a ceU lumen filled and a cell-wall bulked wood polymer composite in water. Wood and Fiber Science, 23(2), 165-172. 

GaUe, J., Schneider, R., Heinloth, A., Dimmeler, S., and Heermeier, K., 1999, Lp(a) and LDL induce apoptosis in human endothelial cells and in rabbit aorta role of oxidative stress. Kidney Int. 55 1450-1461. 

Cuthbert GL, Daujat S, Snowden AW, Erdjument-Bromage H, Hagiwara T, Yamada M, Schneider R, Gregory PD, Tempst P, Bannister AJ, et al (2004) Histone deimination antagonizes arginine methy-lation. Cell 118 545-553... 

Santos-Rosa H, Schneider R, Bernstein BE, Karabetsou N, Morillon A, Weise C, Schreiber SL, Mellor J, Kouzarides T (2003) Methylation of histone H3 K4 mediates association of the Iswlp ATPase with chromatin. Mol Cell 12 1325-1332... 

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