Poly caprolactone Tricalcium Phosphate Scaffolds

The successful modification has been confirmed by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy analysis. Additive manufactured PCL/silanized tricalcium phosphate scaffolds have been fabricated using a screw extrusion system. Testing mechanical properties have demonstrated that both 

In order to enhance the osteoconductive property of the developed PCL/silanized tricalcium phosphate scaffolds, a carbonated hydroxyapatite-gelatin composite has been coated onto the scaffolds using a biomimetic co-precipitation process, which has been characterized by scanning electron microscope (SEM) and XPS. Confocal laser microscopy and SEM images revealed a most uniform distribution of porcine bone marrow stromal cells and cell-sheet accumulation on the carbonated hydroxyapatite-gelatin composite coated PCL/silanized tricalcium phosphate scaffolds (4). 

Purthermore, the reverse transcription polymerase chain reaction and western blot analysis revealed that carbonated hydroxyapatite-gelatin composite coated PCL/silanized tricalcium phosphate scaffolds stimulate an osteogenic differentiation of bone marrow stromal cells the most in comparison to the other scaffolds. In-vitro results of SEM, confocal microscopy and proliferation rate also showed that there is no detrimental effect of the modification on biocompatibility of the scaffolds (4). 

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The feasibility of additive manufactured poly(caprolactone) (PCL) silanized tricalcium phosphate scaffolds coated with carbonated hydroxyapatite-gelatin composite for bone tissue engineering has been tested (4). In order to reinforce the scaffolds to match the mechanical properties of cancellous bone, tricalcium phosphate has been modified with y-glycidoxypropyltrimethoxysilane and incorporated into PCL to synthesize a PCL/silanized tricalcium phosphate composite. y-GlycidoxypropyltrimethoxysUane is shown in Figure 3.1. 

Lei, Y., Rai, B., Ho, K.H., Teoh, S.H., 2007. In vitro degradation of novel bioactive poly-caprolactone-20% tricalcium phosphate composite scaffolds for bone engineering. Materials Science and Engineering C-Biomimetic and Supramolecular Systems 27, 293-298. 

Aydin, H.M., CaUmli, A., Piskin, E., 2004. Micropoious scaffolds from poly (lactide-co-e-caprolactone) composites with hydroxyapatite and tricalcium phosphate using supercritical CO2 for bone tissue engineering. Journal of Bioactive and Compatible Polymers 19,383—394. 

Sezer UA, Arslantunah D, Aksoy EA, Hasirci V, Hasirci N. Poly(epsilon-caprolactone) composite scaffolds loaded with gentamicin-containing beta-tricalcium phosphate/gelatin microspheres for bone tissue engineering applications. J Appl Pol3mi Sci 2014 131(8). 

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