Mass spectrometry quantification

Lorenz SA, Moy MA, Dolan AR, Wood TD. 1999. Electrospray ionization fourier transform mass spectrometry quantification of enkephalin using an internal standard. Rapid Commun Mass Spectrom 13 2098. 

AdoMet and AdoHcy are separated by HPLC and analysed by electrospray ionisation-tandem mass spectrometry. Quantification is based on comparison of the signal from natural AdoMet (transition m/z 399 ->- m/z 250) and AdoHcy (transition m/z 385 ->- m/z 135 and 134) with that from analogous transitions of the stable isotope internal standards. 

Solid certified reference materials (CRMs) are used for calibrating the analytical procedures in inorganic mass spectrometry. Quantification of analytical data in solid mass spectrometry via... 

Roddy T.P., Nelson B.C., Sung C.C., Araghi S., Wilkens D., Zhang X.K., Thomas J.J., Richards S.M., Liquid chromatography-tandem mass spectrometry quantification of globotriaosylceramide in plasma for long-term monitoring of Fabry patients treated with enzyme replacement therapy, Clinical chemistry 51 (2005) 237-240. 

The internal standard method is the most widely used approach in mass spectrometry quantification. The method is especially useful when the amount of the sample introduced and the instrument response may vary from run to run. In this... 

What type of internal standard is most appropriate in mass spectrometry quantification ... 

Wang M, Heo GY, Omarova S, Pikuleva lA, Turko IV (2012) Sample prefractionation for mass spectrometry quantification of low-abundance membrane proteins. Anal Chem 84 5186-5191 Bogdanovic N, Bretillon L, Lund EG, Diczfalusy... 

Gas Chromatograpny-Mass Spectrometry Quantification Method for Simultaneous Determination of Endogenous and Exogenous Human Plasma Testosterone Levels After Treatment with Testosterone Undecanoate, A New Oral Active Testosterone Derivative Adv. Mass Spectrom. 78 1544-1550 (1978) CA 89 103061n... 

Colucci AP, Aventaggiato L, Centrone M, Gagliano-Candela R (2010) Validation of an extraction and gas chromatography-mass spectrometry quantification method for cocaine, methadone, and morphine in postmortem adipose tissue. J Anal Toxicol 34 342-346... 

Stoob, K. et al.. Fully automated online solid phase extraction coupled directly to liquid chromatography-tandem mass spectrometry Quantification of sulfonamide antibiotics, neutral and acidic pesticides at low concentrations in surface waters, /. Chromatogr. A, 1097,138, 2005. 

Desiderio, D. M., and M. Kai Preparation of Stable Isotope-Incorporated Peptide Internal Standards for Field Desorption Mass Spectrometry Quantification of Peptides in Biologic Tissue. Biomed. Mass Spectrom. 10, 471 (1983). 

Analytical Approaches. Different analytical techniques have been appHed to each fraction to determine its molecular composition. As the molecular weight increases, complexity increasingly shifts the level of analytical detail from quantification of most individual species in the naphtha to average molecular descriptions in the vacuum residuum. For the naphtha, classical techniques allow the isolation and identification of individual compounds by physical properties. Gas chromatographic (gc) resolution allows almost every compound having less than eight carbon atoms to be measured separately. The combination of gc with mass spectrometry (gc/ms) can be used for quantitation purposes when compounds are not well-resolved by gc. 

A definitive method for stmctural deterrnination is x-ray crystallography. Extensive x-ray crystal stmcture deterrninations have been done on a wide variety of steroids and these have been collected and Hsted (270). In addition, other analytical methods for steroid quantification or stmcture determination include, mass spectrometry (271), polarography, fluorimetry, radioimmunoassay (264), and various chromatographic techniques (272). 

The mass spectrometer (ms) is a common adjunct to a chromatographic system (see Mass spectrometry). The combination of a gas chromatograph for component separation and a mass spectrometer (gc/ms) for detection and identification of the separated components is a powerful tool, particularly when the data are collected usiag an on-line data-handling system. QuaUtative information inherent ia the separation can be coupled with the identification of stmcture and relatively straightforward quantification of a mixture s components. 

Oba, Y., et al. (2004). Identification of the luciferin-luciferase system and quantification of coelenterazine by mass spectrometry in the deep-see luminous ostracod Conchoecia pseudodiscophora. ChemBioChem 5 1495-1499. 

Nikolai LN, McClure EL, MacLeod SL, Wong CS (2006) Stereoisomer quantification of the Beta-blocker drugs atenolol, metoprolol, and propranolol in wastewaters by chiral high-performance liquid chromatography-tandem mass spectrometry. J Chromatogr A 1131 103-109... 

Knowledge of the identity of phenolic compounds in food facilitates the analysis and discussion of potential antioxidant effects. Thus studies of phenolic compounds as antioxidants in food should usually by accompanied by the identification and quantification of the phenols. Reversed-phase HPLC combined with UV-VIS or electrochemical detection is the most common method for quantification of individual flavonoids and phenolic acids in foods (Merken and Beecher, 2000 Mattila and Kumpulainen, 2002), whereas HPLC combined with mass spectrometry has been used for identification of phenolic compounds (Justesen et al, 1998). Normal-phase HPLC combined with mass spectrometry has been used to identify monomeric and dimeric proanthocyanidins (Lazarus et al, 1999). Flavonoids are usually quantified as aglycones by HPLC, and samples containing flavonoid glycosides are therefore hydrolysed before analysis (Nuutila et al, 2002). 

Weller, P. and Breithaupt, D.E., Identification and quantification of zeaxanthin esters in plants using liquid chromatography-mass spectrometry, J. Agric. Food Chem., 51, 7044, 2003. 

McCann MT, Thompson MM, Gueron IC et al. (1996) Methyl malonic acid quantification by stable isotope dilution gas chromatography-mass spectrometry from filter paper urine samples. Clin Chem 42 9io-9i4. 

A. Fox and R. M. T. Rosario, Quantification of muramic acid, a marker for bacterial peptidoglycan in dust collected from hospital and home air-conditioning filters using gas-chromatography mass spectrometry. Indoor Air-Intemat. J. Air Quality Cl. 4 239 (1994). 

R. Goodacre, Characterisation and quantification of microbial systems using pyrolysis mass spectrometry introducing neural networks to analytical pyrolysis, Microbiol. (Europe), 2 19 (1994). 

The development of new fiber coatings in the near future should further improve the specificity of SPME and overcome some of the observed matrix effects. Quantification by stable isotope dilution gas chromatography/mass spectrometry (GC/MS) may assist in improving analytical performance. Along with the possible application of micro LC and capillary LC columns to in-tube SPME, the development of novel derivatization methods and the potential for the analysis of fumigant pesticides, SPME appears to be a technique with a future in the analysis of pesticide residues in food. 

As a more sensitive detection method, MS can be very useful in amino acid determinations. For example, S-carboxymethyl-(R) cysteine or SCMC, is a mucolytic agent used in the treatment of respiratory diseases. The development of a method utilizing high performance IEC and atmospheric pressure ionization (API) mass spectrometry to quantify SCMC in plasma has been described.66 This method is simple (no derivatization needed), rapid (inn time 16 min.), sensitive (limit of quantification 200 ng/mL in human plasma), and has an overall throughput of more than 60 analyses per day. API-MS was used successfully with IEC to determine other sulfur-containing amino acids and their cyclic compounds in human urine.67 IEC has also been used as a cleanup step for amino acids prior to their derivatization and analysis by gas chromatography (GC), either alone or in conjunction with MS.68 69... 

Anacardio, R., Cantalini, M.G., De Angelis, F., and Gentile, M., Quantification of S-carboxymethyl-(R)-cysteine in human plasma by high-performance ion-exchange liquid chromatography/atmospheric pressure ionization mass spectrometry, /. Mass Spectrom., 32, 388, 1997. 

The main advantages of plasma-source mass spectrometry (PS-MS) over other analytical techniques, such as PS-AES and ETAAS, are the possibilities of quantitative isotope determination and isotope dilution analysis the rapid spectral scanning capability of the mass spectrometer and semiquantitative determinations to within a factor of two or three. Several labelling methods are used for the quantification of analytes present in complex mixtures. In these methods, the sample is spiked... 

Although often used as a qualitative (identification) tool, MS may act as a quantitative inorganic mass detector. Quantification of organic analytes often takes place in combination with chromatography or in tandem MS mode. It should be realised that mass spectrometry is certainly not a panacea for all polymer/additive problems, although it is developing into a major tool for this purpose. 

The HPLC-MS/MS assay was also successfully applied to the measurement of UV-induced dimeric pyrimidine photoproducts [123, 124]. The latter lesions were released from DNA as modified dinucleoside monophosphates due to resistance of the intra-dimer phosphodiester group to the exonuclease activity during the hydrolysis step [125, 126]. The hydrolyzed photoproducts exhibit mass spectrometry and chromatographic features that allow simultaneous quantification of the three main classes of photolesions, namely cyclobutane dimers, (6-4) photoproducts, and Dewar valence isomers, for each of the four possible bipyrimidine sequences. It may be added that these analyses are coupled to UV detection of normal nucleosides in order to correct for the amount of DNA in the sample and obtain a precise ratio of oxidized bases or dimeric photoproducts to normal nucleosides. 

---