Sample prefractionation

SEC in combination with multidimensional liquid chromatography (LC-LC) may be used to carry out polymer/additive analysis. In this approach, the sample is dissolved before injection into the SEC system for prefractionation of the polymer fractions. High-MW components are separated from the additives. The additive fraction is collected, concentrated by evaporation, and injected to a multidimensional RPLC system consisting of two columns of different selectivity. The first column is used for sample prefractionation and cleanup, after which the additive fraction is transferred to the analytical column for the final separation. The total method (SEC, LC-LC) has been used for the analysis of the main phenolic compounds in complex pyrolysis oils with minimal sample preparation [974]. The identification is reliable because three analytical steps (SEC, RPLC and RPLC) with different selectivities are employed. The complexity of pyrolysis oils makes their analysis a demanding task, and careful sample preparation is typically required. 

Zuo, X., Speicher, D. W. (2000). A method for global analysis of complex proteomes using sample prefractionation by solution isoelectrofocusing prior to two-dimensional electrophoresis. Anal Biochem. 284(2), 266-278. 

Zuo, X., Speicher, D.W. (2004). Microscale solution isoelectricfocusing a sample prefractionation method for comprehensive proteome analysis. Methods Mol. Biol. 244, 361-375. 

Gorg A, Boguth G, Kopf A, ReU G, Parlar H, Weiss W. (2002) Sample prefractionation with Sephadex isoelectric focusing prior to narrow pH range two-dimensional gels. Proteomics 2, 1652-7. 

Herbert B, Righetti PG. A turning point in proteome analysis sample prefractionation via multicompartment electrolyzers with isoelectric membranes. Electrophoresis 2000 21 3639-3648. 

Desciibe strategies of sample prefractionation for genomic and proteomic analysis. 

Song Y-A et al (2010) Free-flow ztme electrophoresis of peptides and proteins in PDMS microchip fOT narrow pi range sample prefractionation coupled with mass spectrometry. Anal Chem 82(6) 2317-2325... 

Wang M, Heo GY, Omarova S, Pikuleva lA, Turko IV (2012) Sample prefractionation for mass spectrometry quantification of low-abundance membrane proteins. Anal Chem 84 5186-5191 Bogdanovic N, Bretillon L, Lund EG, Diczfalusy... 

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