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Sensitivity of detection

Figure 7.9 shows a schematic representation of this effect, in which the ratio of the two isotopes changes with time. To obtain an accurate estimate of the ratio of ion abundances, it is better if the relative ion yields decrease linearly (Figure 7.9) which can be achieved by adjusting the filament temperature continuously to obtain the desired linear response. An almost constant response for the isotope ratio can be obtained by slow evaporation of the sample, viz., by keeping the filament temperature as low as is consistent with sufficient sensitivity of detection (Figure 7.9). [Pg.52]

The nebulization concept has been known for many years and is commonly used in hair and paint spays and similar devices. Greater control is needed to introduce a sample to an ICP instrument. For example, if the highest sensitivities of detection are to be maintained, most of the sample solution should enter the flame and not be lost beforehand. The range of droplet sizes should be as small as possible, preferably on the order of a few micrometers in diameter. Large droplets contain a lot of solvent that, if evaporated inside the plasma itself, leads to instability in the flame, with concomitant variations in instrument sensitivity. Sometimes the flame can even be snuffed out by the amount of solvent present because of interference with the basic mechanism of flame propagation. For these reasons, nebulizers for use in ICP mass spectrometry usually combine a means of desolvating the initial spray of droplets so that they shrink to a smaller, more uniform size or sometimes even into small particles of solid matter (particulates). [Pg.106]

The contrast for specimen detail in the field of view is gready enhanced by darkfield illumination (10). The degree of contrast and sensitivity of detection of smaH-object details depend on the relative indices of the specimen and the mounting Hquid and on the intensity of the illumination. Darkfield illumination is not, however, a satisfactory solution for biologists who need direct transmitted light in order to observe specimens, especially stained specimens. It is, however, very usefiil in detecting asbestos fibrils that often exist in door tiles or water and air samples as 20-nm fibers (10 times finer than the resolution of an asbestos analyst s usual microscope) (11). Darkfield illumination yields an uimatural appearance and difficulties in interpretation hence, a need for better contrast methods stiU exists. [Pg.329]

This method of detection is at its most sensitive if the absorption maximum (A a,) of the sample molecule is exactly at the wavelength of the UV light employed for irradiation. The further lies from this the less radiation is absorbed and the lower the sensitivity of detection. If the compound does not absorb at the wavelength of radiation or if it possesses an absorption minimum just there then such components are not detected by this method. Figure 4C illustrates this with the sweeteners saccharin and dulcin as examples. [Pg.10]

Rather than dipping the chromatogram in acid solution it is preferable to heat it to 100°C for 2—5 min (fume cupboard ) in order to evaporate the ammonia and turn the background yellow. By this means it is possible to increase the sensitivity of detection for some of cations e.g. Sr and Ba. However, these zones fade after some time, so that it is necessary to quantify the chromatogram immediately after heating. [Pg.144]

Note Note that the diazotization of primary aromatic amines can also be achieved by placing the chromatogram for 3 — 5 min in a twin-trough chamber containing nitrous fumes (fume cupboard ). The fumes are produced in the empty trough of the chamber by addition of 25% hydrochloric acid to a 20% sodium nitrite solution [2, 4], iV-(l-Naphthyl)ethylenediamine can be replaced in the reagent by a- or -naphthol [10, 14], but this reduces the sensitivity of detection [2]. Spray solutions Ila and lib can also be used as dipping solutions. [Pg.225]

Note The reagent can be employed on silica gel, kieselguhr, RP and, with lower sensitivity of detection, on cellulose layers. The color differentiation is probably greater on cellulose layers [1]. A dark blue background is produced on polyamide layers. [Pg.229]

The sensitivity of detection is usually between 0.2 and 0.5 pg per chromatogram zone. This is also true for pesticides based on organophosphorus acids [9]. [Pg.254]

Fig. 2 The influence of the reagent concentration on the sensitivity of detection detection of equal amounts of luteolin with basic lead acetate solution that was (A) undiluted, (B) diluted 1 + 4 and (C) diluted 1 + 50. Fig. 2 The influence of the reagent concentration on the sensitivity of detection detection of equal amounts of luteolin with basic lead acetate solution that was (A) undiluted, (B) diluted 1 + 4 and (C) diluted 1 + 50.
This ester is formed from the anhydride in pyridine and is quantitatively cleaved with H2NNH2-H2O, Pyr-AcOH. The sensitivity of detection of this ester is high with its absorbance maximum of 513 nm and extinction coefficient of 78,600 in 5% CI2CHCO2H/CH2CI2 where it forms the trityl cation. ... [Pg.169]

Electrodriven separation techniques are destined to be included in many future multidimensional systems, as CE is increasingly accepted in the analytical laboratory. The combination of LC and CE should become easier as vendors work towards providing enhanced microscale pumps, injectors, and detectors (18). Detection is often a problem in capillary techniques due to the short path length that is inherent in the capillary. The work by Jorgenson s group mainly involved fluorescence detection to overcome this limit in the sensitivity of detection, although UV-VIS would be less restrictive in the types of analytes detected. Increasingly sensitive detectors of many types will make the use of all kinds of capillary electrophoretic techniques more popular. [Pg.212]

In analytical LC there are two primary reasons why chemical derivatization of the sample constituents would be necessary, and they are 1) to enhance the separation and 2) to increase the sensitivity of detection. Under certain circumstances, derivatization can also be used to reduce peak asymmetry, i.e. to reduce tailing, or to improve the stability of labile components so that they do not re-arrange or decompose during the chromatographic process. However, sensitivity enhancement is the most common goal of derivatization. For example, aliphatic alcohols that contain no UV chromaphore can be reacted with benzoyl chloride to form a benzoic ester. [Pg.237]

The first part of the book consists of a detailed treatment of the fundamentals of thin-layer chromatography, and of measurement techniques and apparatus for the qualitative and quantitative evaluation of thin-layer chromatograms. In situ prechromatographic derivatization techniques used to improve the selectivity of the separation, to increase the sensitivity of detection, and to enhance the precision of the subsequent quantitative analysis are summarized in numerous tables. [Pg.4]

Note The colors of the chromatogram zones fade relatively quickly. A temperature of 180 °C should not be exceeded in the first heating step, otherwise the sensitivity of detection will be reduced. [Pg.71]

Comparative investigations have revealed that varying the acid used or replacing it by a base is generally accompanied by changes — sometimes drastic - in the sensitivity of detection. The information that follows is intended to help the TLC user to choose and optimize the most suitable chloramine T reagent for a particular application ... [Pg.93]

A further extension of the DFG S19 method was achieved when polar analytes and those unsuitable for GC were determined by LC/MS or more preferably by liquid chromatography/tandem mass spectrometry (LC/MS/MS). Triple-quadrupole MS/MS and ion trap MS" have become more affordable and acceptable in the recent past. These techniques provide multiple analyte methods by employing modes such as time segments, scan events or multiple injections. By improving the selectivity and sensitivity of detection after HPLC separation, the DFG S19 extraction and cleanup scheme can be applied to polar or high molecular weight analytes, and cleanup steps such as Si02 fractionation or even GPC become unnecessary. [Pg.57]

A variety of formats and options for different types of applications are possible in CE, such as micellar electrokinetic chromatography (MEKC), isotachophoresis (ITP), and capillary gel electrophoresis (CGE). The main applications for CE concern biochemical applications, but CE can also be useful in pesticide methods. The main problem with CE for residue analysis of small molecules has been the low sensitivity of detection in the narrow capillary used in the separation. With the development of extended detection pathlengths and special optics, absorbance detection can give reasonably low detection limits in clean samples. However, complex samples can be very difficult to analyze using capillary electrophoresis/ultraviolet detection (CE/UV). CE with laser-induced fluorescence detection can provide an extraordinarily low LOQ, but the analytes must be fluorescent with excitation peaks at common laser wavelengths for this approach to work. Derivatization of the analytes with appropriate fluorescent labels may be possible, as is done in biochemical applications, but pesticide analysis has not been such an important application to utilize such an approach. [Pg.781]

For the El ion source, the generated total ion stream is directly proportional to the gas pressure in the impact field, which provides a basic condition for quantitative analysis. Compounds can only safely be quantified if influences on the sensitivity of detection, such as ion-molecule reactions and competition in the ionisation process, can be excluded by experimental evidence. [Pg.361]

The Af-dansylated amino acid (e.g., glycine, leucine, proline) exhibits a yellow fluorescence. The sensitivity of detection for amino acids by this method is about 10 9 mol of amino acid. The advantage of this prodecure in comparison with that using dansyl chloride is the fact that it can be carried out in homogenous aqueous solution without addition of a cosolvent. 191... [Pg.231]

Mekler, V. (1994). A photochemical technique to enhance sensitivity of detection of fluorescence resonance energy transfer. Photochem. Photo-biol. 59, 615-20. [Pg.71]


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See also in sourсe #XX -- [ Pg.6 , Pg.8 ]

See also in sourсe #XX -- [ Pg.95 ]




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