Lymphocyte cell lines

Another example of vims clearance is for IgM human antibodies derived from human B lymphocyte cell lines where the steps are precipitation, size exclusion using nucleases, and anion-exchange chromatography (24). A second sequence consists of cation-exchange, hydroxylapatite, and immunoaffinity chromatographies. Each three-step sequence utilizes steps based on different properties. The first sequence employs solubiUty, size, and anion selectivity the second sequence is based on cation selectivity, adsorption, and selective recognition based on an anti-u chain IgG (24). 

Low levels of hypochlorous acid can function as a mediator in cell activation, induce NFKB heterodimer p50 /p65 in a T-lymphocytic cell line through proteolysis of IKB and pl05 inhibitors. Hypochlorous acid will also contribute to the release of TNFo in cellular supernatants of T-lymphocytes which are capable of commencing activation in non-induced cells (Schoonbroodt et ah, 1997). Hypochlorous acid can react with amines to produce chloroamines and N-chlorinated derivatives that have a long lifetime in plasma. Taurine, a sulfonated amino acid, will finally combine with these two products to reduce their toxicity. 

Activation of cells results in the release of IKB, followed by the rapid proteolysis of IKB. Although phosphorylation of serine 32 and 36 in the amino-terminal part of IKBa occurs when the proinflammatory cytokines or mitogens are administered to a T lymphocytic cell line, a different site of action has been found after H2O2 incubation (Schoonbroodt et al., 2000). The tyrosine residue 42 and the C-terminal PEST (Pro-Glu-Ser-Thr) domain plays a major role in the phosphorlylation of IKB after treatment with H2O2. Furthermore the CVinducible phosphorylation was not dependent upon IKB kinase activation but involved casein kinase II. The importance of iron for the activation of NFKB was underlined by the fact that... 

Finke et al.54 BJAB cells (B-lymphocytic cell line) 1% SDS + proteinase K Housekeeping gene PBGD (porphobilinogen deaminase) Most routine FFPE tissues will be available for RNA analysis. 

The thiazoloimidazopyridine derivatives 451 were tested for their effect on human immunodeficiency virus (HIV)-induced cytopathogenicity in a human T4-lymphocyte cell line and showed a reproducible anti-HIV activity that caused a 50% or greater reduction of viral cytophatic effect <1994FA345>. Finally, imidazo[2,1 -/dthiazolone 489 was found to have an antitumor activity and proved to be effective against brain tumor cell lines <2002JHC1133>. 

A hybridoma is a cell line created by the fusion of two different lymphocyte cell lines, one of which is derived from a tumour. 

Gil. Gilliand, B. C., Ford, D. K., and Mannik, M., Synthesis by an established lymphocyte cell line from a rheumatoid synovium. Arthritis Rheum. 21, 330-336 (1978). 

Arai, H., Tsou, C.-F., and Charo, I. F. (1997) Chemotaxis in a lymphocyte cell line transfected with C-C chemokine receptor 2B Evidence that directed migration is mediated by bg dimers released by activation of Gai-coupled receptors. Proc. Natl. Acad. Sci. USA 94, 14,495-14,499. 

In order to validate the hypothesis that microparticles are structures enriched in plasma membrane proteins, we have analysed the proteome composition of different microparticle preparations obtained from a T-Lymphocytic cell line. Such microparticles can be produced and enriched in vitro by mitogenic activation (PHA) or apoptosis induction (Act Dl, TNFo ) for instance. Microparticles protein mixtures obtained from the two stimulations were separated independently on a ID gel. The different gels were cut each 2 mm and the different slides were digested and analyzed by nanoLC-MS/MS. 

Steinitz, M., Klem, G., Koskimies, S., and Makel, O. (1977) EB virus-induced B lymphocyte cell lines producing specific antibody. Nature 269, 420. 

HTLV-I can be consistently infected into rabbits, rats and squirrel monkeys (Lairmore et al., 2005). HTLV-I infectivity for rabbits was first reported by using intravenous inoculations of the MT-2 cell line (Akagi et al., 1985), a T-cell leukemia cell line established from a patient with ATL, and with the Ra-1 cell line (Miyoshi etal., 1985), a rabbit lymphocyte cell line derived from cocultivation of rabbit lymphocytes with MT-2 cells. The rabbit model has provided important information regarding the immune response against HTLV-1 infection (Cockerell et al., 1990). Establishment of a rabbit model of clinical HTLV-1 disease has been more problematic. In the majority of studies, rabbit infection has paralleled the asymptomatic infection of humans. Sporadic clinical lymphoprolif-erative disorders in HTLV-1-infected rabbits were reported (Simpson et al., 1996 Kindt et al., 2000 Zhao et al., 2002). In each of these cases, clinical disease developed after one year and usually several years after the initial infection. 

Suzuki S, Chuang LE, Doi RH, Bidlack JM, Chuang RY (2001) Kappa-opioid receptors on lymphocytes of a human lymphocytic cell line morphine-induced up-regulation as evidenced by competitive RT-PCR and indirect immunofluorescence. Int Immuno-pharmacol 1 1733-1742. 

Mizrahi A (1975) Plutonic polyols in human lymphocyte cell line cultures. Journal of Clinical Microbiology 2 11-13. 

Bost and Pascual, 1992). In lymphocytes and lymphocytic cell lines, substance P av pients mitogen-induced proliferation of B and T lymphocytes (Payan et O.I., 1983 Bost and Pascual, 1992), stimulates immunoglobulin synthesis (Bost and Pascual, 1992), augments the release of interleukin 2 (11 2) from concanavalin A (Con A)-stimulated murine T cells (Rameshwar et al.,... 

Inoue, K. Ikawa, S. Semba, K. Sukegawa, J. Yamamoto, T. Toyoshima, K. Isolation and sequencing of cDNA clones homologous to the v-fgr oncogene from a human B lymphocyte cell line, IM-9. Oncogene, 1, 301-304... 

Holladay FP, Heitz T, Chen YL, Chiga M, Wood GW. Successful treatment of a malignant rat glioma with cytotoxic T lymphocytes. Neurosurgery 1992 31 528-533. Kitahara T, Watanabe O, Yamaura A, Makino H, Watanabe T, Suzuki G et al. Establishment of interleukin 2 dependent cytotoxic T lymphocyte cell line specific for autologous brain tumor and its intracranial administration for therapy of the tumor. J Neurooncol 1987 4 329-336. 

In nonneutrophil cell lines transfected with chemoattractant receptors, chemotaxis induced by leukocyte chemoattractants is mediated by the released Gjp. subunits, not G [16, 266, 267]. Gells (human embryonic kidney cells and a lymphocyte cell line) transfected vwth chemoattractant receptors migrate in response to the corresponding chemoattractant,... 

Because human immunodeficiency virus 1 (HIV-1) is consistently associated with AIDS (9-12), we investigated the action of ascorbate on HIV infection under controlled conditions in vitro. Here, we report the effects of ascorbate on acutely and chronically HIV-infected T-lymphocytic cell lines grown continuously in the presence of nontoxic concentrations of the compound. In addition, we report the action of ascorbate on cell-free virus particles in vitro. 

Recent work from the laboratories of Berger (16) and Jacobson (127) demonstrated that lymphocyte cell lines derived from normal donors typically increased poly(ADP-ribose) synthesis in response to V-methyl-V -nitro-nitrosoguanidine or UV irradiation. These treatments also stimulated unscheduled DNA synthesis. In contrast, longterm lymphocyte cell lines derived from patients with xeroderma pigmentosum and known to be defective in their ability to repair UV-induced damage showed a normal to exaggerated poly(ADP-ribose)... 

Further investigations of ascorbate inhibition of virus replication have been carried out using retroviruses as models. Bissell et al. (1980), working with the avian retrovirus of chickens, found that while cell-free virus was resistant to ascorbate inactivation upon short-term treatment in vitro, exposure of virus-infected cultures to the vitamin resulted in reduction of virus replication and lowered infec-tivity of newly replicated virus. A subsequent study found that ascorbate interfered with the replication and cell-transforming potential of Rous sarcoma virus by stabilizing the differentiated state of chicken cells (Schwarz, 1991). In a lymphocytic cell line latently infected with human T-cell leukemia virus (HTLV-1), ascorbate was shown to interfere with virus production triggered by chemical inducers added to the culture medium (Blakeslee et al., 1985). 

Durrieu, C., Degraeve, R, Chappaz, S., Martial-Gros, A. (2006). Immunomodulating effects of water-soluble extracts of traditional French Alps cheeses on a hnman T-lymphocyte cell line. International Dairy Journal, 16, 1505-1514. 

Haley PJ, Finch GL, Hoover MD, et al. Immunologic specificity of lymphocyte cell lines fiom dogs exposed to BO. In Thomassen DG, Shyr LI, Bechtold WE, et al., eds. Inhalation Toxicology Research Institute Annual Report 1989 1990, LMF-129. Springfield National Technical Information Service 1990 236-239. 

W.-S. Cheng, R. E. Tarone, A. D. Andrews, J. S. Whang-Peng, and J. H. Robbins, Ultraviolet light-induced sister chromatid exchanges in xeroderma pigmentosum and in Cockayne s syndrome lymphocyte cell lines. Cancer Res. 38, 1601-1609 (1978). 

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