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Liquid chromatography—mass hormones

Penning TM, Lee S, Jin Y, Gutierrez A, Blair IA (2010) Liquid chromatography-mass spectrometry (LC-MS) of teroid hormone metabolites and its applications. J Steroid Biochem Mol Biol 121 546-555... [Pg.282]

See also Chemometrics and Statistics Optimization Strategies. Chromatography Overview Principies. Derivatization of Analytes. Extraction Soivent Extraction Principies Solid-Phase Extraction. Forensic Sciences Systematic Drug Identification. Gas Chromatography Mass Spectrometry. Hormones Steroids. Liquid Chromatography Liquid Chromatography-Mass Spectrometry. Mass Spectrometry Atmospheric Pressure Ionization Techniques Forensic Applications. [Pg.1655]

Takahashi, S., and Murata, T. Microanalysis of Thyroid Hormones of Serum and Urine by Mass Fragmentography Using Gas-Liquid Chromatography-Mass Spectrometry Nippon Naibumpi Gakkai Zasshi 52(2) 149-157 (1976) CA 84 161370v... [Pg.107]

Wu, S., Jardine, I.,Hancock, W., and Karger, B. (2004) A new and sensitive on-line liquid chromatography/mass spectrometric approach for top-down protein analysis The comprehensive analysis of human growth hormone in an E. coU lysate using a hybrid linear ion trap/Fourier transform ion cyclotron resonance mass spectrometer. Rapid Communications in Mass Spectrometry, 18,2201-2207. [Pg.269]

Combalbert S, Pype ML, Bemet N, Hemandez-Raquet G (2010) Enhanced methods for conditioning, storage, and extraction of liquid and solid samples of manure for determination of steroid hormones by solid-phase extraction and gas chromatography-mass spectrometry. Anal Bioanal Chem 398 973-984... [Pg.108]

Liquid chromatography-(tandem) mass spectrometry of selected emerging pollutants (steroid sex hormones, drugs and alkyl phenolic surfactants) in the aquatic environment [56]... [Pg.16]

Draisci R, Pallaeschi L, Ferretti E, Lucentini L, Cammarata P. 2000. Quantitation of anabolic hormones and their metabolites in bovine serum and urine by liquid chromatography-tandem mass spectrometry. J Chromatogr A 870(1-2) 511-522. [Pg.37]

The number of detectors that are sensitive and selective enough to be applied online with LC is limited because the solvents used are not compatible, as in the case of immunochemical detection after reversed- or normal-phase LC. The technology of coupling is still under development and not yet available in a large number of laboratories not specialized in techniques such as LC-MS. Therefore, LC separations are frequently followed by offline detection. Confirmatory analysis of suspected liquid chromatographic peaks can be made possible by coupling liquid chromatography with mass spectrometry. Atmospheric-pressure chemical ionization LC-MS has been employed for the identification of six steroid hormones in bovine tissues (448). [Pg.1065]

Chang, J. P Kiehl, D. E. Kennington, A. 1997. Separation and characterization of the tryptic peptide mapping of recombinant bovine growth hormone by reversed-phase high-performance liquid chromatography electrospray mass spectrometry. Rapid Commun. Mass Spectrom., 11, 1266-1270. [Pg.210]

Liquid chromatography thermospray—mass spectrometry has been utilized to determine the identity and purity of taxol, solvent-induced degradation of cloxacillin [129], benzodiazepines, and ethanolamine-type antihistamines [130]. Electrospray LC-MS has been used for peptide mapping of the human growth hormone [131]. [Pg.336]

EPA EPA Chemical QC Guidelines EPA method 539 Determination of hormones in drinking water by solid phase extraction (SPE) and liquid chromatography electrospray ionization tandem mass spectrometry (LC-MS/MS) [77]... [Pg.272]

Schlusener MP, Bester K (2005) Determination of steroid hormones, hormone conjugates and macrolide antibiotics in influents and effluents of sewage treatment plants utilizing high-performance liquid chromatography/tandem mass spectrometry with electrospray and atmospheric pressure chemical ionization. Rapid Commun Mass Spectrom 19 3269-3278... [Pg.281]

Ingrand V, Herry G, Beausse J, de Roubin M (2003) Analysis of steroid hormones in effluents of wastewater treatment plants by liquid chromatography-tandem mass spectrometry. J Chromatogr A 1020 99-104... [Pg.284]

EEC [9], which lays down the methods to be used for detecting residues of substances having a hormonal or a thyreostatic action (immunoassay, thin-layer chromatography, liquid chromatography, gas chromatography, mass spectrometry, spectrometry or other methods),... [Pg.161]

Constanzer, M. Chavez-Eng, C. Matuszewski, B. Determination of a Thermally Labile Metabolite of a Novel Growth Hormone Secretagogue in Human and Dog Plasma by Liquid Chromatography with Ion Spray Tandem Mass Spectrometric Detection, J. Chromatogr. B 760,45-53 (2001). [Pg.500]

Chang, Y.-C., Li, C.-M., Li, L.-A., Jong, S.-B., Liao, P.C. and Chang, L.W. (2003). Quantitative measurement of male steroid hormones using automated on-line solid phase extraction liquid chromatography-tandem mass spectrometry and comparison with radioimmunoassay. Analyst, 128, 363-368. [Pg.29]

METHOD FOR THE DETERMINATION OF SOME SELECTED STEROID HORMONES AND BISPHENOL A IN WATER AT LOW NG/L LEVEL BY ON-LINE SOLID-PHASE EXTRACTION COMBINED WITH LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY... [Pg.379]

Lopez de Alda, M. and Barcelo, D., Determination of steroid sex hormones and related synthetic compounds considered as endocrine disrupters in water hy liquid chromatography-diode array detection-mass spectrometry, J. Chromatogr. A, 892, 391-406, 2000. [Pg.1267]

A number of suitable immunoassays and radioimmunoassays are available for measuring the major hormones testosterone, estrogen, LH, and FSH. Some of these assays may need adjustments to ensure that the majority of values fall within the optimal part of the measuring range because the ranges differ between laboratory animal species. Given the similarities in the structures of FSH and LH, some assays can be used for both rats and mice if the antisera are suitable (Jarrell et al. 1988). A number of methods have been described that use gas liquid or liquid chromatography combined with mass spectrometry, which can be used to measure several of the steroid hormones. The development of various contraceptives has often been helped by these hormone measurements as biomarkers of efficacy rather than markers of toxicity in animal models. [Pg.236]

In species where pheromones are not typical hormones, intensive chemical analyses have been productive in search of pheromone compounds. Such analyses usually involve exhaustive chromatographic and spectromefric methods including high performance liquid chromatography (HPLC), mass spectrometry (MS), and nuclear magnetic resonance (NMR) to concentrate, purify and identify compounds in water conditioned by mature animals, and to confirm the pheromone functions through EOG and/or behavioral tests. Chemical analysis has been used successfully... [Pg.472]

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See also in sourсe #XX -- [ Pg.396 , Pg.397 ]



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